Rubrobacter radiotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Rubrobacteria; Rubrobacterales; Rubrobacteraceae; Rubrobacter

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023X6K6|A0A023X6K6_9ACTN Beta-lactamase class A OS=Rubrobacter radiotolerans OX=42256 GN=RradSPS_2347 PE=4 SV=1
MM1 pKa = 7.17IAAIAMLAFVGGCGSLVQGGGQEE24 pKa = 4.04QQGGSGQQQEE34 pKa = 4.4QQQGQQGGEE43 pKa = 3.84DD44 pKa = 3.58TQQQEE49 pKa = 4.21QQASGGEE56 pKa = 4.31TTQEE60 pKa = 3.94QQGGEE65 pKa = 4.08QGAQAPEE72 pKa = 4.53DD73 pKa = 3.86PTLSLSIPSLNKK85 pKa = 10.23NIEE88 pKa = 4.45NIPTGRR94 pKa = 11.84GDD96 pKa = 4.93DD97 pKa = 3.92EE98 pKa = 5.37QLLTDD103 pKa = 3.79NAAIHH108 pKa = 5.67VFPTGFPWQEE118 pKa = 3.64GANTYY123 pKa = 10.34LAGHH127 pKa = 5.63VEE129 pKa = 5.4GYY131 pKa = 9.86PGTPSYY137 pKa = 11.06KK138 pKa = 10.09AFEE141 pKa = 4.38GLRR144 pKa = 11.84EE145 pKa = 4.05LQNGDD150 pKa = 4.03EE151 pKa = 5.13IIITDD156 pKa = 3.83ANGTEE161 pKa = 3.93YY162 pKa = 10.2TYY164 pKa = 11.13EE165 pKa = 3.98VFEE168 pKa = 4.68GRR170 pKa = 11.84VIDD173 pKa = 3.91PTDD176 pKa = 3.39TSVLDD181 pKa = 3.83PVPGRR186 pKa = 11.84SIVTLQTCEE195 pKa = 3.88IVEE198 pKa = 4.27VNPDD202 pKa = 3.33GTPNYY207 pKa = 10.18SDD209 pKa = 3.33TEE211 pKa = 4.13RR212 pKa = 11.84YY213 pKa = 8.76IVQGEE218 pKa = 4.29LVSS221 pKa = 3.66

Molecular weight: 23.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023X753|A0A023X753_9ACTN Probable glycine dehydrogenase (decarboxylating) subunit 1 OS=Rubrobacter radiotolerans OX=42256 GN=gcvPA PE=3 SV=1
MM1 pKa = 7.6SEE3 pKa = 3.94RR4 pKa = 11.84SNEE7 pKa = 3.96RR8 pKa = 11.84GGGRR12 pKa = 11.84LAGALARR19 pKa = 11.84KK20 pKa = 9.36SGRR23 pKa = 11.84AVAGSATGTGDD34 pKa = 4.06LAPVRR39 pKa = 11.84AASRR43 pKa = 11.84PGVAGGGVRR52 pKa = 11.84SRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84TSGPSRR62 pKa = 11.84TRR64 pKa = 11.84SADD67 pKa = 3.26YY68 pKa = 9.04KK69 pKa = 10.72QCMAHH74 pKa = 5.89IRR76 pKa = 11.84KK77 pKa = 9.36DD78 pKa = 3.11VRR80 pKa = 11.84EE81 pKa = 4.05RR82 pKa = 11.84VEE84 pKa = 3.88EE85 pKa = 3.9ARR87 pKa = 11.84HH88 pKa = 4.76NRR90 pKa = 11.84EE91 pKa = 3.21VRR93 pKa = 11.84AVLEE97 pKa = 4.08EE98 pKa = 4.11DD99 pKa = 3.37LRR101 pKa = 11.84AAGILFKK108 pKa = 10.56PGNPEE113 pKa = 3.35YY114 pKa = 10.65SALVEE119 pKa = 4.03LLLVQWLEE127 pKa = 4.04NVGFGPEE134 pKa = 3.9DD135 pKa = 3.13

Molecular weight: 14.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3148

0

3148

976227

30

2566

310.1

33.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.135 ± 0.059

0.71 ± 0.012

5.162 ± 0.033

8.312 ± 0.053

3.631 ± 0.027

9.739 ± 0.043

1.672 ± 0.019

3.777 ± 0.034

2.656 ± 0.031

10.441 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.912 ± 0.019

2.206 ± 0.021

5.141 ± 0.032

2.198 ± 0.03

8.26 ± 0.051

5.769 ± 0.03

4.91 ± 0.024

8.739 ± 0.04

1.12 ± 0.017

2.507 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski