Escherichia phage PGN829.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; unclassified Gamaleyavirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385II99|A0A385II99_9CAUD Putative HNH homing endonuclease OS=Escherichia phage PGN829.1 OX=2315696 PE=4 SV=1
MM1 pKa = 7.55PMKK4 pKa = 10.23AFHH7 pKa = 6.66VEE9 pKa = 3.72TDD11 pKa = 3.42SLQEE15 pKa = 3.68AVKK18 pKa = 10.35IKK20 pKa = 10.73NALADD25 pKa = 3.69YY26 pKa = 11.36DD27 pKa = 3.97LFQYY31 pKa = 9.97EE32 pKa = 4.46NNVKK36 pKa = 10.47GDD38 pKa = 3.57YY39 pKa = 11.2ANANGIEE46 pKa = 4.12MWDD49 pKa = 3.81EE50 pKa = 4.06SLTDD54 pKa = 3.41QDD56 pKa = 4.35LVDD59 pKa = 4.61MEE61 pKa = 5.72LEE63 pKa = 4.24DD64 pKa = 4.73RR65 pKa = 11.84WVDD68 pKa = 2.92WFYY71 pKa = 11.79EE72 pKa = 4.22DD73 pKa = 3.85GNEE76 pKa = 4.18YY77 pKa = 10.56FDD79 pKa = 6.04DD80 pKa = 4.54PEE82 pKa = 5.46EE83 pKa = 4.17YY84 pKa = 10.79LDD86 pKa = 3.71WLATQSS92 pKa = 3.26

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385II74|A0A385II74_9CAUD Uncharacterized protein OS=Escherichia phage PGN829.1 OX=2315696 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84TLCNRR7 pKa = 11.84YY8 pKa = 9.46NSQKK12 pKa = 9.35MINMLQPWGSKK23 pKa = 9.75ISLNARR29 pKa = 11.84WQVSVEE35 pKa = 4.16DD36 pKa = 4.58KK37 pKa = 10.98NGQHH41 pKa = 6.8HH42 pKa = 6.93DD43 pKa = 3.49HH44 pKa = 6.67FFVAKK49 pKa = 7.46PTRR52 pKa = 11.84KK53 pKa = 9.58QIRR56 pKa = 11.84KK57 pKa = 7.37LHH59 pKa = 5.52KK60 pKa = 9.68VV61 pKa = 3.12

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

22236

42

3450

264.7

29.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.513 ± 0.592

0.913 ± 0.15

5.891 ± 0.131

6.548 ± 0.241

3.481 ± 0.158

6.741 ± 0.262

1.718 ± 0.141

5.644 ± 0.248

6.57 ± 0.305

8.122 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.901 ± 0.186

5.81 ± 0.14

4.106 ± 0.152

4.268 ± 0.325

4.407 ± 0.166

6.152 ± 0.191

6.377 ± 0.278

6.782 ± 0.247

1.214 ± 0.113

3.841 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski