Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal))

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Sugarcane streak virus

Average proteome isoelectric point is 7.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q89822|REPA_SSVN Replication-associated protein A OS=Sugarcane streak virus (isolate South Africa) OX=268781 GN=C1 PE=3 SV=1
MM1 pKa = 6.92STVGSTVSSTPSRR14 pKa = 11.84RR15 pKa = 11.84FKK17 pKa = 10.63HH18 pKa = 6.37RR19 pKa = 11.84NVNTFLTYY27 pKa = 10.36SRR29 pKa = 11.84CPLEE33 pKa = 4.17PEE35 pKa = 4.25AVGLHH40 pKa = 5.28IWSLIAHH47 pKa = 6.26WTPVYY52 pKa = 10.2VLSVRR57 pKa = 11.84EE58 pKa = 3.86THH60 pKa = 6.75EE61 pKa = 4.49DD62 pKa = 3.07GGYY65 pKa = 9.93HH66 pKa = 5.24IHH68 pKa = 6.87VLAQSAKK75 pKa = 9.59PVYY78 pKa = 8.36TTDD81 pKa = 3.66SGFFDD86 pKa = 3.91IDD88 pKa = 3.45GFHH91 pKa = 7.45PNIQSAKK98 pKa = 8.75SANKK102 pKa = 8.73VRR104 pKa = 11.84AYY106 pKa = 10.8AMKK109 pKa = 10.84NPVTYY114 pKa = 9.5WEE116 pKa = 4.69RR117 pKa = 11.84GTFIPRR123 pKa = 11.84KK124 pKa = 7.95TSFLGDD130 pKa = 3.47STEE133 pKa = 4.28PNSKK137 pKa = 9.98KK138 pKa = 9.84QSKK141 pKa = 10.53DD142 pKa = 3.27DD143 pKa = 3.36IVRR146 pKa = 11.84DD147 pKa = 4.03IIEE150 pKa = 4.43HH151 pKa = 5.04STNKK155 pKa = 9.66QEE157 pKa = 4.09YY158 pKa = 10.37LSMIQKK164 pKa = 10.03ALPYY168 pKa = 9.73EE169 pKa = 4.28WATKK173 pKa = 9.84LQYY176 pKa = 10.43FEE178 pKa = 4.96YY179 pKa = 10.26SANKK183 pKa = 9.66LFPDD187 pKa = 3.01IQEE190 pKa = 4.71IYY192 pKa = 9.95TSPFPQSTPALLDD205 pKa = 3.23PTAINTWLEE214 pKa = 3.83NNLYY218 pKa = 9.77QVSPSAYY225 pKa = 7.96MLANPSCLTLEE236 pKa = 4.43EE237 pKa = 4.36ATSDD241 pKa = 4.45LIWMHH246 pKa = 5.23EE247 pKa = 4.23TSRR250 pKa = 11.84TLIPTGSSACTSSGQQEE267 pKa = 4.42QASPPGPGAWEE278 pKa = 4.6DD279 pKa = 3.73IITGRR284 pKa = 11.84TTSTGPPTTRR294 pKa = 11.84TQNTTSSTTYY304 pKa = 9.97PSSTVHH310 pKa = 6.56AGSNN314 pKa = 3.37

Molecular weight: 34.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q89551|CAPSD_SSVN Capsid protein OS=Sugarcane streak virus (isolate South Africa) OX=268781 GN=V1 PE=3 SV=1
MM1 pKa = 8.08DD2 pKa = 4.02SFGRR6 pKa = 11.84APPLWPQSALPRR18 pKa = 11.84VPGAAPSSSGLPWSRR33 pKa = 11.84VGEE36 pKa = 3.84IAIFTFVAVLALYY49 pKa = 9.37LLWSWVGRR57 pKa = 11.84DD58 pKa = 3.44LLLVLKK64 pKa = 10.23ARR66 pKa = 11.84RR67 pKa = 11.84GGTTEE72 pKa = 4.03EE73 pKa = 4.3LTFGPRR79 pKa = 11.84EE80 pKa = 4.06RR81 pKa = 11.84HH82 pKa = 5.67SLPAVAVARR91 pKa = 11.84VEE93 pKa = 4.46NPPCPSGSVEE103 pKa = 3.83ARR105 pKa = 11.84PFTGG109 pKa = 3.94

Molecular weight: 11.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1035

109

364

258.8

28.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.763 ± 0.727

1.353 ± 0.215

4.251 ± 0.604

4.928 ± 0.488

3.768 ± 0.223

6.377 ± 1.536

2.609 ± 0.317

5.024 ± 0.577

5.121 ± 0.92

6.667 ± 0.943

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.449 ± 0.103

4.444 ± 0.817

7.246 ± 0.886

3.382 ± 0.561

5.507 ± 0.99

9.758 ± 0.799

8.502 ± 1.268

6.087 ± 0.969

2.512 ± 0.244

4.251 ± 0.79
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski