Sporosarcina sp. HYO08

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina; unclassified Sporosarcina

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2889 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A135W703|A0A135W703_9BACL acidPPc domain-containing protein OS=Sporosarcina sp. HYO08 OX=1759557 GN=AU377_08135 PE=4 SV=1
MM1 pKa = 7.6ALSLMNINVNVTGGSARR18 pKa = 11.84FFNVLSADD26 pKa = 3.67LAVAKK31 pKa = 10.46DD32 pKa = 3.88VAISAADD39 pKa = 3.68FVDD42 pKa = 5.58DD43 pKa = 4.12MGNDD47 pKa = 2.99ITEE50 pKa = 4.69FPAATNGYY58 pKa = 9.06YY59 pKa = 10.53NFYY62 pKa = 10.55INGVLQEE69 pKa = 4.67DD70 pKa = 4.46GSYY73 pKa = 11.18NITTTEE79 pKa = 3.85LTFNDD84 pKa = 3.31VTGTLTAGTPLIVQVVSHH102 pKa = 5.55VTTVV106 pKa = 2.62

Molecular weight: 11.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A135W700|A0A135W700_9BACL NADPH-dependent 7-cyano-7-deazaguanine reductase OS=Sporosarcina sp. HYO08 OX=1759557 GN=queF PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.46SGRR28 pKa = 11.84RR29 pKa = 11.84ILAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.84GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2889

0

2889

865762

42

1570

299.7

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.884 ± 0.053

0.647 ± 0.013

5.161 ± 0.032

7.499 ± 0.061

4.534 ± 0.04

7.156 ± 0.043

2.108 ± 0.02

7.7 ± 0.048

6.248 ± 0.041

9.638 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.022

3.964 ± 0.036

3.734 ± 0.024

3.727 ± 0.034

4.312 ± 0.034

5.695 ± 0.029

5.651 ± 0.031

7.27 ± 0.032

0.954 ± 0.016

3.214 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski