Shuangao sobemo-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEH8|A0A1L3KEH8_9VIRU RNA-directed RNA polymerase OS=Shuangao sobemo-like virus 3 OX=1923476 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 6.14EE3 pKa = 3.96IYY5 pKa = 11.07SPVVWNLPDD14 pKa = 3.92NYY16 pKa = 10.51LSYY19 pKa = 11.57DD20 pKa = 3.66SFVKK24 pKa = 10.21VCYY27 pKa = 10.4GLDD30 pKa = 3.56FRR32 pKa = 11.84SSPGVPYY39 pKa = 10.38CYY41 pKa = 10.02DD42 pKa = 3.6YY43 pKa = 9.32PTVGDD48 pKa = 3.61WFHH51 pKa = 7.11RR52 pKa = 11.84KK53 pKa = 9.36ALDD56 pKa = 3.51LSEE59 pKa = 4.39QRR61 pKa = 11.84LQILWLDD68 pKa = 3.69VQDD71 pKa = 5.57LINGKK76 pKa = 9.47LDD78 pKa = 4.44PILRR82 pKa = 11.84YY83 pKa = 9.27FIKK86 pKa = 10.58RR87 pKa = 11.84EE88 pKa = 3.62PHH90 pKa = 5.42KK91 pKa = 10.54KK92 pKa = 10.1KK93 pKa = 10.7KK94 pKa = 9.48IAQKK98 pKa = 9.52RR99 pKa = 11.84WRR101 pKa = 11.84LIASTPLNVQVLWHH115 pKa = 6.09MLFDD119 pKa = 4.09FQNDD123 pKa = 3.43KK124 pKa = 10.91EE125 pKa = 4.24IEE127 pKa = 4.14QVYY130 pKa = 10.14SIPSKK135 pKa = 10.31QGMSLVHH142 pKa = 5.8GQWKK146 pKa = 9.58FYY148 pKa = 9.77KK149 pKa = 10.15RR150 pKa = 11.84QWKK153 pKa = 9.86SKK155 pKa = 9.69GFNFCVDD162 pKa = 3.39AEE164 pKa = 4.26AWDD167 pKa = 3.36WTMPYY172 pKa = 10.25WLILWDD178 pKa = 3.57LDD180 pKa = 3.09FRR182 pKa = 11.84YY183 pKa = 10.65RR184 pKa = 11.84MGRR187 pKa = 11.84GRR189 pKa = 11.84KK190 pKa = 7.26MDD192 pKa = 3.42EE193 pKa = 3.58WRR195 pKa = 11.84LLALNLIQYY204 pKa = 7.67MFEE207 pKa = 4.32KK208 pKa = 10.64PLVCLSDD215 pKa = 3.41GSLYY219 pKa = 10.48RR220 pKa = 11.84QLLAGIMKK228 pKa = 9.72SGCVNTISTNSHH240 pKa = 4.93GQIMDD245 pKa = 4.55HH246 pKa = 6.89IIVCVRR252 pKa = 11.84EE253 pKa = 4.04NLPIYY258 pKa = 8.62PLPDD262 pKa = 3.3ACGDD266 pKa = 3.46DD267 pKa = 4.07KK268 pKa = 11.61YY269 pKa = 11.42QNEE272 pKa = 4.14INAGSVEE279 pKa = 4.33AFSKK283 pKa = 10.46IGVKK287 pKa = 9.85IKK289 pKa = 10.79LMEE292 pKa = 5.02KK293 pKa = 8.44GHH295 pKa = 6.68DD296 pKa = 4.21FIGHH300 pKa = 5.49DD301 pKa = 4.03HH302 pKa = 6.47SLEE305 pKa = 4.34SGPVPLYY312 pKa = 10.31FDD314 pKa = 3.22KK315 pKa = 11.18HH316 pKa = 5.74LFSYY320 pKa = 10.62CIEE323 pKa = 4.15KK324 pKa = 10.79PEE326 pKa = 3.9FLSEE330 pKa = 4.17FLEE333 pKa = 4.67SMCFLYY339 pKa = 9.74THH341 pKa = 5.95SEE343 pKa = 4.19KK344 pKa = 10.8FSFWYY349 pKa = 10.27DD350 pKa = 3.08LALKK354 pKa = 10.27HH355 pKa = 5.87NSRR358 pKa = 11.84VKK360 pKa = 10.14SRR362 pKa = 11.84FYY364 pKa = 10.62YY365 pKa = 10.41KK366 pKa = 10.73YY367 pKa = 9.74MYY369 pKa = 9.36DD370 pKa = 3.25IPVAA374 pKa = 3.79

Molecular weight: 44.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEH8|A0A1L3KEH8_9VIRU RNA-directed RNA polymerase OS=Shuangao sobemo-like virus 3 OX=1923476 PE=4 SV=1
MM1 pKa = 7.64AFLSSVLSKK10 pKa = 10.83INWSTVVPVAFSIANTAAVAYY31 pKa = 7.17FACQGTTSVVVIPEE45 pKa = 4.33EE46 pKa = 4.1VPSWKK51 pKa = 9.97NRR53 pKa = 11.84FSLSSIIPYY62 pKa = 9.54FEE64 pKa = 3.76KK65 pKa = 10.05QVPIRR70 pKa = 11.84RR71 pKa = 11.84VAITCCGVYY80 pKa = 9.74VFWFCYY86 pKa = 8.82RR87 pKa = 11.84TGLFSKK93 pKa = 9.95FFSFLRR99 pKa = 11.84YY100 pKa = 7.24MAPGFNYY107 pKa = 9.72IKK109 pKa = 10.52FKK111 pKa = 10.43LTGHH115 pKa = 5.94PEE117 pKa = 3.27IRR119 pKa = 11.84IEE121 pKa = 4.01IDD123 pKa = 2.97PTEE126 pKa = 4.03TSTRR130 pKa = 11.84IIQEE134 pKa = 4.17SIRR137 pKa = 11.84AGSTEE142 pKa = 4.03SAANPPHH149 pKa = 5.71FQCMIGYY156 pKa = 9.01VDD158 pKa = 3.77SGVFTAVGAAIRR170 pKa = 11.84IHH172 pKa = 7.06DD173 pKa = 4.15VLVMPRR179 pKa = 11.84HH180 pKa = 6.23VYY182 pKa = 8.7DD183 pKa = 3.23QCMEE187 pKa = 4.01RR188 pKa = 11.84PAAYY192 pKa = 10.16GNRR195 pKa = 11.84TKK197 pKa = 11.07NIVNLVNFEE206 pKa = 4.63CIDD209 pKa = 3.64MDD211 pKa = 4.0TDD213 pKa = 5.0LIMVKK218 pKa = 6.85TTKK221 pKa = 10.43DD222 pKa = 3.08ALATIGMAIPSIQNCIPIAGTNVNIVGIEE251 pKa = 3.99NAGTTGNLQLSEE263 pKa = 4.52VNFGCTVYY271 pKa = 10.72HH272 pKa = 6.26GTTKK276 pKa = 10.34PGYY279 pKa = 9.93SGAAYY284 pKa = 9.53FAGPRR289 pKa = 11.84LVGMHH294 pKa = 5.47SHH296 pKa = 6.92GGPLNGGYY304 pKa = 9.46SASFILAMVNHH315 pKa = 6.84FLSKK319 pKa = 10.27IPEE322 pKa = 4.71DD323 pKa = 3.63SDD325 pKa = 3.03KK326 pKa = 11.09WLAQQFRR333 pKa = 11.84AGKK336 pKa = 9.36EE337 pKa = 3.67IKK339 pKa = 10.01FDD341 pKa = 4.25LGWGSLDD348 pKa = 3.47TEE350 pKa = 4.95RR351 pKa = 11.84IQIGGEE357 pKa = 3.31FHH359 pKa = 7.0IVQKK363 pKa = 10.27RR364 pKa = 11.84SCVSAIGKK372 pKa = 10.01DD373 pKa = 3.67YY374 pKa = 11.2GNHH377 pKa = 5.41VNDD380 pKa = 4.97DD381 pKa = 4.08GYY383 pKa = 11.83LKK385 pKa = 10.76FKK387 pKa = 10.92FDD389 pKa = 3.99GQYY392 pKa = 10.81QDD394 pKa = 5.07YY395 pKa = 10.8SDD397 pKa = 4.21EE398 pKa = 4.4DD399 pKa = 3.73SSRR402 pKa = 11.84GRR404 pKa = 11.84ARR406 pKa = 11.84SRR408 pKa = 11.84RR409 pKa = 11.84GRR411 pKa = 11.84KK412 pKa = 8.47IKK414 pKa = 10.35RR415 pKa = 11.84NRR417 pKa = 11.84FRR419 pKa = 11.84SEE421 pKa = 3.83SPINEE426 pKa = 3.82SAIPKK431 pKa = 9.17NEE433 pKa = 3.6KK434 pKa = 10.27SRR436 pKa = 11.84GSNTSGKK443 pKa = 9.92SEE445 pKa = 3.89ASADD449 pKa = 3.66QLINEE454 pKa = 4.5SLRR457 pKa = 11.84VEE459 pKa = 4.23LMNLISSAIRR469 pKa = 11.84SSMKK473 pKa = 8.56RR474 pKa = 11.84TAYY477 pKa = 10.06RR478 pKa = 11.84KK479 pKa = 10.0SPPTPNTNPSNSTPNTSS496 pKa = 2.78

Molecular weight: 54.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

870

374

496

435.0

49.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.517 ± 1.434

2.184 ± 0.138

5.287 ± 1.034

4.828 ± 0.157

5.402 ± 0.132

6.552 ± 0.914

2.529 ± 0.422

7.241 ± 0.678

6.207 ± 0.961

7.241 ± 1.979

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.759 ± 0.279

4.828 ± 0.839

4.943 ± 0.081

3.218 ± 0.326

5.402 ± 0.532

8.736 ± 0.942

4.138 ± 1.574

6.092 ± 0.296

2.069 ± 0.874

4.828 ± 0.987
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski