Bacillus sp. 7586-K

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A268E3J6|A0A268E3J6_9BACI Asparagine synthase (Glutamine-hydrolyzing) OS=Bacillus sp. 7586-K OX=2021690 GN=asnB PE=3 SV=1
MM1 pKa = 7.06LQVAVFDD8 pKa = 4.42EE9 pKa = 4.36EE10 pKa = 4.44HH11 pKa = 6.65EE12 pKa = 4.34KK13 pKa = 11.16DD14 pKa = 4.16LEE16 pKa = 4.12EE17 pKa = 4.92EE18 pKa = 4.05INAFLAEE25 pKa = 4.49LDD27 pKa = 4.59DD28 pKa = 4.23IQVKK32 pKa = 9.97DD33 pKa = 3.13IRR35 pKa = 11.84YY36 pKa = 9.49SVTLTIDD43 pKa = 3.15EE44 pKa = 5.24DD45 pKa = 4.39GEE47 pKa = 4.35QIYY50 pKa = 10.45CYY52 pKa = 10.05SALVLYY58 pKa = 9.31QII60 pKa = 5.11

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A268E2Q5|A0A268E2Q5_9BACI Sugar ABC transporter substrate-binding protein OS=Bacillus sp. 7586-K OX=2021690 GN=CHH83_19040 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLSAA44 pKa = 4.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5220

0

5220

1467317

19

2173

281.1

31.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.299 ± 0.038

0.73 ± 0.01

5.073 ± 0.029

7.696 ± 0.038

4.685 ± 0.029

6.445 ± 0.032

2.077 ± 0.017

8.458 ± 0.039

7.356 ± 0.028

9.751 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.018

4.928 ± 0.029

3.387 ± 0.02

3.693 ± 0.022

3.743 ± 0.023

6.141 ± 0.029

5.442 ± 0.025

6.638 ± 0.031

1.069 ± 0.015

3.797 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski