Vibrio phage 2E1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9LW00|A0A1C9LW00_9CAUD Uncharacterized protein OS=Vibrio phage 2E1 OX=1873904 PE=4 SV=1
MM1 pKa = 6.8TLKK4 pKa = 10.71VVDD7 pKa = 5.31PINITEE13 pKa = 4.31AVLTASDD20 pKa = 3.9IPEE23 pKa = 4.61PDD25 pKa = 3.13ASVGEE30 pKa = 4.58VEE32 pKa = 4.24WQDD35 pKa = 3.29PSVLGRR41 pKa = 11.84FGNLPDD47 pKa = 3.48VQYY50 pKa = 10.46TVVLGSDD57 pKa = 3.6GFLYY61 pKa = 10.94SFGRR65 pKa = 11.84AGAVVKK71 pKa = 10.34IDD73 pKa = 4.2PDD75 pKa = 3.79TSTMTTFGSYY85 pKa = 10.19NVGRR89 pKa = 11.84VYY91 pKa = 10.97AAVLAGDD98 pKa = 3.81GNIYY102 pKa = 10.81AVGSGSAVLKK112 pKa = 10.9LEE114 pKa = 4.48ISTQTLSTFGSYY126 pKa = 10.78AGTCEE131 pKa = 4.17SATLAPDD138 pKa = 3.46GDD140 pKa = 4.34IYY142 pKa = 11.33CVSTNGFVLKK152 pKa = 10.08IDD154 pKa = 3.93VSAQTTSQFGSYY166 pKa = 9.59GAGYY170 pKa = 7.83KK171 pKa = 9.47TSSLGGDD178 pKa = 3.51GNIYY182 pKa = 10.39CAGSQGDD189 pKa = 3.84VLKK192 pKa = 10.51IDD194 pKa = 3.61VTEE197 pKa = 4.13QTVSTFGSVTGKK209 pKa = 10.9YY210 pKa = 9.19YY211 pKa = 11.17GSAQGVDD218 pKa = 3.26GNVYY222 pKa = 10.61CIGYY226 pKa = 9.18SGNVLKK232 pKa = 10.9INVSNQSVSTFGSCVGAFEE251 pKa = 5.27SAALDD256 pKa = 3.37ANGRR260 pKa = 11.84IKK262 pKa = 10.64CVASSSTTASGDD274 pKa = 3.12ILEE277 pKa = 5.15IDD279 pKa = 3.7TASEE283 pKa = 3.88SVYY286 pKa = 10.76RR287 pKa = 11.84FGSYY291 pKa = 9.32TDD293 pKa = 3.58NFFSIALAPNGKK305 pKa = 8.45MFAVGNDD312 pKa = 3.37VGSSTGIIEE321 pKa = 4.57ILDD324 pKa = 3.85TYY326 pKa = 11.52SPGDD330 pKa = 3.42RR331 pKa = 11.84VALEE335 pKa = 4.35SEE337 pKa = 4.13HH338 pKa = 7.38LVYY341 pKa = 10.66QCLSVTYY348 pKa = 9.34QNPKK352 pKa = 9.96VGATEE357 pKa = 5.07DD358 pKa = 3.81STATWIVVGPTNKK371 pKa = 8.94WAMFDD376 pKa = 3.56GLQNTKK382 pKa = 9.54TSSSTDD388 pKa = 3.5FTITLNPITYY398 pKa = 9.99VNTLAMFGFSGVEE411 pKa = 3.89SVRR414 pKa = 11.84IEE416 pKa = 3.89VDD418 pKa = 2.61NSLDD422 pKa = 3.39VNIYY426 pKa = 10.7DD427 pKa = 3.92KK428 pKa = 10.39TYY430 pKa = 11.17SMSDD434 pKa = 3.1FSAIYY439 pKa = 10.32DD440 pKa = 3.39HH441 pKa = 6.09YY442 pKa = 10.06TYY444 pKa = 11.22VFYY447 pKa = 10.88QIASLDD453 pKa = 3.82DD454 pKa = 5.39FIATDD459 pKa = 5.33LPPLPNTTIRR469 pKa = 11.84VTFSGSNMTIGEE481 pKa = 4.67LVTGFAIDD489 pKa = 3.28IGQLVAEE496 pKa = 4.52STKK499 pKa = 10.54SDD501 pKa = 3.04RR502 pKa = 11.84FRR504 pKa = 11.84YY505 pKa = 9.58RR506 pKa = 11.84EE507 pKa = 3.62QEE509 pKa = 4.14YY510 pKa = 11.23NEE512 pKa = 4.02FGYY515 pKa = 8.4PTGEE519 pKa = 3.75APIVVEE525 pKa = 4.1LNTYY529 pKa = 10.43DD530 pKa = 3.81VLVPKK535 pKa = 10.58LNNQAIQKK543 pKa = 10.39LLDD546 pKa = 3.59TLTGEE551 pKa = 3.95NTLWVGDD558 pKa = 3.63IGGGQSLVTYY568 pKa = 10.19GLFEE572 pKa = 4.75RR573 pKa = 11.84SPIPYY578 pKa = 10.38SMPNHH583 pKa = 6.54INYY586 pKa = 9.94QITVRR591 pKa = 11.84ASVV594 pKa = 2.9

Molecular weight: 63.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9LVU1|A0A1C9LVU1_9CAUD Uncharacterized protein OS=Vibrio phage 2E1 OX=1873904 PE=4 SV=1
MM1 pKa = 7.45WLISFKK7 pKa = 10.29GLRR10 pKa = 11.84MNNVFRR16 pKa = 11.84MPKK19 pKa = 9.49LHH21 pKa = 6.82RR22 pKa = 11.84NSAAMGKK29 pKa = 9.15ILKK32 pKa = 10.13DD33 pKa = 3.1IEE35 pKa = 4.17KK36 pKa = 10.34RR37 pKa = 11.84GCFYY41 pKa = 11.35YY42 pKa = 10.84NNQRR46 pKa = 11.84VKK48 pKa = 10.83LDD50 pKa = 3.52EE51 pKa = 5.34ANTKK55 pKa = 9.7VDD57 pKa = 3.2GVYY60 pKa = 10.67VYY62 pKa = 10.77LAYY65 pKa = 9.68TPAA68 pKa = 5.34

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11377

68

1142

199.6

22.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.612 ± 0.628

1.406 ± 0.217

6.311 ± 0.208

6.777 ± 0.341

3.964 ± 0.231

6.909 ± 0.356

1.828 ± 0.252

6.267 ± 0.209

6.803 ± 0.414

7.682 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.971 ± 0.202

5.344 ± 0.171

3.068 ± 0.257

3.911 ± 0.366

4.158 ± 0.288

7.304 ± 0.343

6.056 ± 0.328

6.452 ± 0.281

1.292 ± 0.146

3.885 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski