Entomoplasma somnilux

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae; Entomoplasma

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8NYB5|A0A2K8NYB5_9MOLU Uncharacterized protein OS=Entomoplasma somnilux OX=215578 GN=ESOMN_v1c03950 PE=4 SV=1
MM1 pKa = 7.79INNLDD6 pKa = 4.04FNSEE10 pKa = 3.88NYY12 pKa = 10.03IFFNDD17 pKa = 3.88YY18 pKa = 10.28KK19 pKa = 11.21NVMINAFGIGCSLCGAAEE37 pKa = 4.37IIYY40 pKa = 9.48VLKK43 pKa = 10.77GSPKK47 pKa = 10.21PIGSFLNDD55 pKa = 3.04VNGNLTDD62 pKa = 3.69QEE64 pKa = 4.15IEE66 pKa = 4.11KK67 pKa = 10.56LIEE70 pKa = 4.05KK71 pKa = 10.12PITDD75 pKa = 3.37WQNFEE80 pKa = 5.27DD81 pKa = 4.07NNSEE85 pKa = 3.97KK86 pKa = 10.61FIPTFICSEE95 pKa = 4.31CWNSSQEE102 pKa = 3.91

Molecular weight: 11.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8P2D5|A0A2K8P2D5_9MOLU Uracil phosphoribosyltransferase OS=Entomoplasma somnilux OX=215578 GN=upp PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.52VKK11 pKa = 9.98HH12 pKa = 5.34AHH14 pKa = 3.94THH16 pKa = 4.95GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.84NGRR28 pKa = 11.84RR29 pKa = 11.84VIKK32 pKa = 10.29ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

725

0

725

264357

29

1811

364.6

41.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.721 ± 0.098

0.511 ± 0.023

5.226 ± 0.064

6.681 ± 0.105

5.218 ± 0.082

5.525 ± 0.088

1.367 ± 0.032

10.07 ± 0.083

9.775 ± 0.095

9.16 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.043

7.455 ± 0.096

2.6 ± 0.04

3.328 ± 0.045

2.72 ± 0.054

6.324 ± 0.07

5.454 ± 0.072

5.866 ± 0.063

1.211 ± 0.043

3.547 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski