Bacillus caeni

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5R9F5G4|A0A5R9F5G4_9BACI Uncharacterized protein OS=Bacillus caeni OX=2574798 GN=FCL54_10580 PE=4 SV=1
MM1 pKa = 7.5SLEE4 pKa = 3.82WFDD7 pKa = 3.9RR8 pKa = 11.84VSGEE12 pKa = 4.11LQDD15 pKa = 4.16HH16 pKa = 6.79LEE18 pKa = 4.52SICDD22 pKa = 3.34KK23 pKa = 10.24YY24 pKa = 11.58DD25 pKa = 3.46QVGHH29 pKa = 5.36MSIDD33 pKa = 3.19RR34 pKa = 11.84GAKK37 pKa = 8.39HH38 pKa = 6.11PRR40 pKa = 11.84IEE42 pKa = 4.85FYY44 pKa = 11.36VEE46 pKa = 3.52TDD48 pKa = 3.24DD49 pKa = 5.79DD50 pKa = 3.9GRR52 pKa = 11.84EE53 pKa = 4.12YY54 pKa = 10.75FCSLFYY60 pKa = 10.83DD61 pKa = 3.55PHH63 pKa = 7.59NEE65 pKa = 3.62EE66 pKa = 5.7FYY68 pKa = 10.93TEE70 pKa = 5.26SYY72 pKa = 11.38DD73 pKa = 4.7LDD75 pKa = 4.19LEE77 pKa = 4.54HH78 pKa = 7.16TSRR81 pKa = 11.84TILTDD86 pKa = 3.1IDD88 pKa = 4.02DD89 pKa = 5.7LIDD92 pKa = 3.8AVHH95 pKa = 6.58EE96 pKa = 4.39SFHH99 pKa = 9.03DD100 pKa = 3.68YY101 pKa = 10.62MNEE104 pKa = 3.29EE105 pKa = 4.66DD106 pKa = 4.28IYY108 pKa = 11.6VEE110 pKa = 4.25NEE112 pKa = 3.77EE113 pKa = 5.68LYY115 pKa = 10.42MDD117 pKa = 5.3DD118 pKa = 4.23EE119 pKa = 5.29YY120 pKa = 11.04IDD122 pKa = 3.56EE123 pKa = 5.34GYY125 pKa = 11.01EE126 pKa = 3.76LDD128 pKa = 3.96NEE130 pKa = 4.34FEE132 pKa = 3.96EE133 pKa = 6.0GYY135 pKa = 10.54YY136 pKa = 10.63EE137 pKa = 4.13EE138 pKa = 5.95DD139 pKa = 3.36YY140 pKa = 11.62SEE142 pKa = 5.47AIEE145 pKa = 4.61NEE147 pKa = 4.14IEE149 pKa = 4.37VEE151 pKa = 4.21WITPEE156 pKa = 3.69VTAYY160 pKa = 10.27SHH162 pKa = 6.49EE163 pKa = 4.67DD164 pKa = 3.62EE165 pKa = 5.43VNVTYY170 pKa = 10.78QFGVVQEE177 pKa = 4.33TGDD180 pKa = 3.95GILRR184 pKa = 11.84RR185 pKa = 11.84FNRR188 pKa = 11.84VITTEE193 pKa = 3.95DD194 pKa = 3.27SLIEE198 pKa = 4.4DD199 pKa = 3.7EE200 pKa = 5.37SNFIFSKK207 pKa = 11.09DD208 pKa = 3.31EE209 pKa = 4.3AGTIISMIASNMDD222 pKa = 3.55SLSDD226 pKa = 3.67FDD228 pKa = 5.72FEE230 pKa = 4.7

Molecular weight: 27.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5R9EXD2|A0A5R9EXD2_9BACI Elongation factor Tu OS=Bacillus caeni OX=2574798 GN=tuf PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 8.74PTFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.37VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4545

0

4545

1304964

25

1771

287.1

32.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.875 ± 0.039

0.672 ± 0.01

5.205 ± 0.027

7.933 ± 0.052

4.549 ± 0.038

7.113 ± 0.037

1.993 ± 0.02

7.505 ± 0.034

7.164 ± 0.041

9.651 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.016

4.517 ± 0.024

3.711 ± 0.019

3.456 ± 0.024

4.092 ± 0.028

6.011 ± 0.027

5.221 ± 0.025

7.08 ± 0.029

1.067 ± 0.015

3.481 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski