Circovirus-like genome DCCV-8

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHD6|A0A190WHD6_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-8 OX=1788448 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.86CDD4 pKa = 3.65KK5 pKa = 11.14RR6 pKa = 11.84DD7 pKa = 3.57TGSALSPEE15 pKa = 4.49TLGHH19 pKa = 6.53LNCPTEE25 pKa = 3.91LHH27 pKa = 6.3GVSDD31 pKa = 4.04SQSVEE36 pKa = 3.89NQDD39 pKa = 3.31ISTGKK44 pKa = 8.99YY45 pKa = 6.74WCPSPPRR52 pKa = 11.84KK53 pKa = 9.25PLPKK57 pKa = 10.17SKK59 pKa = 10.65LPSDD63 pKa = 4.16YY64 pKa = 9.96KK65 pKa = 11.33GCMLKK70 pKa = 10.55PPVPLLPRR78 pKa = 11.84LTCIKK83 pKa = 10.32KK84 pKa = 10.3KK85 pKa = 10.98LEE87 pKa = 4.08TEE89 pKa = 4.28TPSNSDD95 pKa = 3.27QEE97 pKa = 4.26QSTGIMQLTGTEE109 pKa = 4.05LKK111 pKa = 10.03SWRR114 pKa = 11.84KK115 pKa = 8.55QVTSIWSQRR124 pKa = 11.84TFTFVIIAPLLQLQAIMKK142 pKa = 9.74SLSEE146 pKa = 3.99LRR148 pKa = 11.84KK149 pKa = 8.69MSSFFMAEE157 pKa = 3.82QVRR160 pKa = 11.84EE161 pKa = 4.2SLGEE165 pKa = 3.89RR166 pKa = 11.84GKK168 pKa = 11.0KK169 pKa = 9.12PDD171 pKa = 3.47QVPILKK177 pKa = 9.53IHH179 pKa = 6.52AANFGVATALTNMLLSMNLEE199 pKa = 4.32VVSISPTCCDD209 pKa = 2.7GWIGIQSALRR219 pKa = 11.84SKK221 pKa = 10.82GAADD225 pKa = 3.54PP226 pKa = 4.33

Molecular weight: 24.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHK4|A0A190WHK4_9CIRC Replication-associated protein OS=Circovirus-like genome DCCV-8 OX=1788448 PE=4 SV=1
MM1 pKa = 7.36SVTVTKK7 pKa = 10.56IAGDD11 pKa = 3.77YY12 pKa = 10.5INRR15 pKa = 11.84SMGNNPYY22 pKa = 10.8AFAYY26 pKa = 10.23RR27 pKa = 11.84GAALGKK33 pKa = 10.01RR34 pKa = 11.84YY35 pKa = 10.05SDD37 pKa = 2.88WVVRR41 pKa = 11.84MGKK44 pKa = 9.82KK45 pKa = 9.89SSSKK49 pKa = 10.07RR50 pKa = 11.84SYY52 pKa = 8.91RR53 pKa = 11.84TNFSMPGKK61 pKa = 10.16VGSAPSGGYY70 pKa = 9.7GSAPGRR76 pKa = 11.84VTGSAPQKK84 pKa = 10.27ALLMLSEE91 pKa = 4.8LPSTSVHH98 pKa = 5.81RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84NSKK104 pKa = 9.73IYY106 pKa = 10.23KK107 pKa = 9.58

Molecular weight: 11.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

971

107

375

242.8

27.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.106 ± 0.742

1.442 ± 0.464

4.84 ± 0.57

5.046 ± 0.794

3.605 ± 0.644

7.518 ± 0.674

1.545 ± 0.242

5.046 ± 0.537

7.518 ± 0.883

7.003 ± 1.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.541

4.016 ± 0.514

5.561 ± 0.674

3.708 ± 0.557

7.003 ± 0.943

7.827 ± 1.591

6.076 ± 0.51

7.312 ± 1.232

1.648 ± 0.794

3.605 ± 0.821
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski