Lactobacillus phage CL2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Colunavirus; Lactobacillus virus CL2

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0HRI2|A0A0P0HRI2_9CAUD HNH endonuclease OS=Lactobacillus phage CL2 OX=1739608 GN=CL2_3 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.24LYY4 pKa = 10.93LVVCEE9 pKa = 4.94TGDD12 pKa = 3.44ADD14 pKa = 3.39QWEE17 pKa = 5.09GGTGEE22 pKa = 4.24ADD24 pKa = 3.04AVFATTDD31 pKa = 3.2KK32 pKa = 11.58AKK34 pKa = 10.8LDD36 pKa = 4.55DD37 pKa = 4.0YY38 pKa = 11.27LSTRR42 pKa = 11.84IYY44 pKa = 10.9GHH46 pKa = 6.95DD47 pKa = 3.25NVVTMEE53 pKa = 4.23LDD55 pKa = 3.3KK56 pKa = 10.9EE57 pKa = 4.35YY58 pKa = 11.3HH59 pKa = 6.55EE60 pKa = 4.75GTKK63 pKa = 9.93SAKK66 pKa = 10.08CLASWWEE73 pKa = 4.28EE74 pKa = 4.51GPCDD78 pKa = 5.12DD79 pKa = 6.23DD80 pKa = 4.59PMEE83 pKa = 4.1II84 pKa = 4.47

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0IZD5|A0A0P0IZD5_9CAUD Integrase OS=Lactobacillus phage CL2 OX=1739608 GN=CL2_24 PE=4 SV=1
MM1 pKa = 7.31SKK3 pKa = 10.62VGIGYY8 pKa = 9.45RR9 pKa = 11.84IQLKK13 pKa = 10.61GMDD16 pKa = 4.07KK17 pKa = 10.84LVAGLLKK24 pKa = 9.97RR25 pKa = 11.84AKK27 pKa = 9.4MDD29 pKa = 3.34VVKK32 pKa = 10.79QIVKK36 pKa = 9.63QQTAQLQTRR45 pKa = 11.84AQQMAGTVYY54 pKa = 10.78AHH56 pKa = 6.42PTGATKK62 pKa = 10.42RR63 pKa = 11.84GIKK66 pKa = 10.04LAITDD71 pKa = 3.83SGLTGIVAMTQEE83 pKa = 4.3YY84 pKa = 10.78NPYY87 pKa = 8.69TEE89 pKa = 3.9NGTRR93 pKa = 11.84FMRR96 pKa = 11.84ARR98 pKa = 11.84PVLKK102 pKa = 10.0PAFLYY107 pKa = 10.49QKK109 pKa = 9.96IQFINQLKK117 pKa = 9.42QAAKK121 pKa = 10.35

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11728

25

1029

195.5

21.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.569 ± 0.517

0.631 ± 0.144

6.941 ± 0.463

5.167 ± 0.369

3.794 ± 0.264

7.427 ± 0.413

1.816 ± 0.234

6.199 ± 0.25

6.872 ± 0.43

7.41 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.174

4.937 ± 0.284

3.402 ± 0.214

4.289 ± 0.231

4.238 ± 0.367

6.924 ± 0.386

6.514 ± 0.345

6.6 ± 0.293

1.731 ± 0.204

3.922 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski