Wenzhou tombus-like virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH28|A0A1L3KH28_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 7 OX=1923677 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84SVLLGTDD9 pKa = 3.04SDD11 pKa = 4.0NVVITKK17 pKa = 7.62EE18 pKa = 4.05FPVFYY23 pKa = 10.12SGYY26 pKa = 9.65PFTDD30 pKa = 3.99DD31 pKa = 3.93NVSTQTDD38 pKa = 3.84VVPIEE43 pKa = 4.11VDD45 pKa = 3.33LARR48 pKa = 11.84AMNEE52 pKa = 4.04SLFSYY57 pKa = 10.57SPGISIDD64 pKa = 3.4NRR66 pKa = 11.84HH67 pKa = 5.48TPRR70 pKa = 11.84YY71 pKa = 9.01DD72 pKa = 3.81QIDD75 pKa = 3.5ATLWQWLCPPRR86 pKa = 11.84LRR88 pKa = 11.84GARR91 pKa = 11.84LLLSNRR97 pKa = 11.84EE98 pKa = 3.86TYY100 pKa = 10.32AYY102 pKa = 9.79EE103 pKa = 4.0LLKK106 pKa = 11.0ALNDD110 pKa = 5.02PISEE114 pKa = 4.6TIDD117 pKa = 3.13EE118 pKa = 4.55ALYY121 pKa = 10.85KK122 pKa = 10.44HH123 pKa = 6.43GNLIKK128 pKa = 9.81TAVDD132 pKa = 3.17YY133 pKa = 10.99LYY135 pKa = 10.41TFRR138 pKa = 11.84ATMPGVQFTHH148 pKa = 7.34DD149 pKa = 3.91KK150 pKa = 11.53DD151 pKa = 3.87EE152 pKa = 4.16MGMLIVRR159 pKa = 11.84ADD161 pKa = 3.01AWISIKK167 pKa = 10.65LKK169 pKa = 9.81EE170 pKa = 4.17VLIEE174 pKa = 4.03RR175 pKa = 11.84PNFRR179 pKa = 11.84GMHH182 pKa = 4.49MQFIKK187 pKa = 10.38MIFLLLCQQKK197 pKa = 10.55SFLDD201 pKa = 3.73EE202 pKa = 4.09MSSDD206 pKa = 4.11LYY208 pKa = 11.03HH209 pKa = 7.4SRR211 pKa = 11.84TLSLNARR218 pKa = 11.84RR219 pKa = 11.84RR220 pKa = 11.84RR221 pKa = 11.84NGYY224 pKa = 9.59RR225 pKa = 11.84KK226 pKa = 9.93YY227 pKa = 10.32PWLGWFSPLANRR239 pKa = 11.84PEE241 pKa = 4.14PSRR244 pKa = 11.84AA245 pKa = 3.29

Molecular weight: 28.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH28|A0A1L3KH28_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 7 OX=1923677 PE=4 SV=1
MM1 pKa = 7.74TYY3 pKa = 10.38QPGIDD8 pKa = 3.47VLVRR12 pKa = 11.84AISEE16 pKa = 3.86RR17 pKa = 11.84LYY19 pKa = 11.03CINTPNGWIQPLVPKK34 pKa = 10.63RR35 pKa = 11.84EE36 pKa = 4.25DD37 pKa = 3.37VSGLALLFDD46 pKa = 5.0AARR49 pKa = 11.84QHH51 pKa = 6.56IRR53 pKa = 11.84VQHH56 pKa = 5.73PMSAEE61 pKa = 3.83QFANCYY67 pKa = 7.26TGPRR71 pKa = 11.84KK72 pKa = 9.46RR73 pKa = 11.84RR74 pKa = 11.84YY75 pKa = 8.74LAAARR80 pKa = 11.84SLAEE84 pKa = 4.37SGISHH89 pKa = 6.34QDD91 pKa = 2.74ARR93 pKa = 11.84LKK95 pKa = 11.03YY96 pKa = 8.17FLKK99 pKa = 10.79FEE101 pKa = 4.45TYY103 pKa = 10.93NFDD106 pKa = 3.79EE107 pKa = 5.16KK108 pKa = 10.94PNPSPRR114 pKa = 11.84GINPRR119 pKa = 11.84SDD121 pKa = 3.23RR122 pKa = 11.84YY123 pKa = 10.28LVEE126 pKa = 4.48LGRR129 pKa = 11.84FLHH132 pKa = 6.6SIEE135 pKa = 3.71KK136 pKa = 10.14RR137 pKa = 11.84IYY139 pKa = 9.5KK140 pKa = 10.37AIALVFGHH148 pKa = 5.91PVIMKK153 pKa = 9.79GYY155 pKa = 8.59NQAQRR160 pKa = 11.84GAILDD165 pKa = 3.79KK166 pKa = 11.23LMSEE170 pKa = 4.15VDD172 pKa = 3.99DD173 pKa = 4.56PVAIPADD180 pKa = 3.37ASRR183 pKa = 11.84FEE185 pKa = 3.85QSIDD189 pKa = 3.38IPHH192 pKa = 7.03LEE194 pKa = 4.6VEE196 pKa = 4.64HH197 pKa = 6.19KK198 pKa = 10.0WYY200 pKa = 11.19LEE202 pKa = 3.7FFRR205 pKa = 11.84GEE207 pKa = 4.1DD208 pKa = 3.3RR209 pKa = 11.84KK210 pKa = 10.53HH211 pKa = 6.04LAFLLDD217 pKa = 3.79LQKK220 pKa = 10.9HH221 pKa = 5.41NKK223 pKa = 9.16GSAHH227 pKa = 6.87ASDD230 pKa = 4.52GDD232 pKa = 4.04LKK234 pKa = 11.16FSIDD238 pKa = 3.96GKK240 pKa = 10.98RR241 pKa = 11.84MSGDD245 pKa = 3.34KK246 pKa = 9.43NTSSGNCIISASLWYY261 pKa = 10.06EE262 pKa = 3.7FAKK265 pKa = 10.6EE266 pKa = 4.12KK267 pKa = 10.65GWLTLTRR274 pKa = 11.84FFCDD278 pKa = 2.83GDD280 pKa = 4.06DD281 pKa = 3.38AVMIIPRR288 pKa = 11.84KK289 pKa = 8.01YY290 pKa = 10.55LKK292 pKa = 10.36EE293 pKa = 3.87FQQDD297 pKa = 3.78YY298 pKa = 11.32LVFCSKK304 pKa = 10.58RR305 pKa = 11.84GFRR308 pKa = 11.84MKK310 pKa = 10.28LDD312 pKa = 3.29KK313 pKa = 10.69VACILEE319 pKa = 4.32QIDD322 pKa = 4.0FCQSRR327 pKa = 11.84PVWTPQGYY335 pKa = 9.97VMVRR339 pKa = 11.84NPVSVSKK346 pKa = 10.47DD347 pKa = 3.53CHH349 pKa = 6.41SKK351 pKa = 11.29APINNEE357 pKa = 3.49KK358 pKa = 10.03IARR361 pKa = 11.84RR362 pKa = 11.84WIKK365 pKa = 10.68AVGLCGLSCNGGIPVMQEE383 pKa = 3.72FYY385 pKa = 10.97RR386 pKa = 11.84AMIRR390 pKa = 11.84YY391 pKa = 9.27AGDD394 pKa = 3.23VKK396 pKa = 11.11ALDD399 pKa = 3.77LTHH402 pKa = 7.19PLMVRR407 pKa = 11.84QFRR410 pKa = 11.84YY411 pKa = 9.66KK412 pKa = 10.01VYY414 pKa = 10.59GMDD417 pKa = 3.4RR418 pKa = 11.84EE419 pKa = 4.05EE420 pKa = 5.04DD421 pKa = 3.72VIHH424 pKa = 6.5PRR426 pKa = 11.84TRR428 pKa = 11.84ASYY431 pKa = 9.82AVAFNVSPSEE441 pKa = 3.76QLLLEE446 pKa = 3.91KK447 pKa = 10.76HH448 pKa = 5.36MAEE451 pKa = 3.9SHH453 pKa = 7.08LEE455 pKa = 3.95FTFEE459 pKa = 4.07TRR461 pKa = 11.84PSITRR466 pKa = 11.84IGLMM470 pKa = 3.96

Molecular weight: 54.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

715

245

470

357.5

41.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.853 ± 0.63

1.678 ± 0.477

6.294 ± 0.357

5.455 ± 0.308

4.895 ± 0.224

5.175 ± 0.604

2.797 ± 0.418

6.154 ± 0.243

5.594 ± 0.836

9.51 ± 1.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.217 ± 0.252

3.636 ± 0.472

5.315 ± 0.221

3.497 ± 0.353

7.972 ± 0.106

6.853 ± 0.273

3.776 ± 1.071

5.455 ± 0.308

1.538 ± 0.278

4.336 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski