Pseudomonas phage LKA5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Hollowayvirus; unclassified Hollowayvirus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1T4N7|A0A0U1T4N7_9CAUD Portal protein OS=Pseudomonas phage LKA5 OX=1327940 GN=LKA5_040 PE=4 SV=1
MM1 pKa = 6.67MWTYY5 pKa = 11.07RR6 pKa = 11.84EE7 pKa = 3.91RR8 pKa = 11.84HH9 pKa = 5.13NRR11 pKa = 11.84AAFNCAQQAWDD22 pKa = 4.29RR23 pKa = 11.84EE24 pKa = 4.2VDD26 pKa = 4.05PLWDD30 pKa = 3.65QPDD33 pKa = 4.07PEE35 pKa = 6.11PEE37 pKa = 4.25DD38 pKa = 4.22EE39 pKa = 4.26EE40 pKa = 5.61QEE42 pKa = 5.18DD43 pKa = 3.93EE44 pKa = 6.16DD45 pKa = 4.33GLQRR49 pKa = 4.66

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1UNM5|A0A0U1UNM5_9CAUD RyR domain-containing protein OS=Pseudomonas phage LKA5 OX=1327940 GN=LKA5_045 PE=4 SV=1
MM1 pKa = 7.11NRR3 pKa = 11.84VHH5 pKa = 7.09IKK7 pKa = 9.55TFASEE12 pKa = 4.23HH13 pKa = 5.19GQSRR17 pKa = 11.84AAALLGITQGALSKK31 pKa = 10.51ALRR34 pKa = 11.84VGRR37 pKa = 11.84DD38 pKa = 2.95IYY40 pKa = 10.31VTAHH44 pKa = 6.91ADD46 pKa = 3.47GTFTAEE52 pKa = 4.16EE53 pKa = 4.5VRR55 pKa = 11.84TFPAQKK61 pKa = 10.15VRR63 pKa = 11.84FVSS66 pKa = 3.52

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

20209

39

3863

306.2

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.504 ± 0.599

0.881 ± 0.202

6.2 ± 0.273

6.279 ± 0.278

2.855 ± 0.152

8.001 ± 0.337

1.999 ± 0.196

4.102 ± 0.275

3.85 ± 0.257

8.214 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.395 ± 0.157

3.004 ± 0.142

5.567 ± 0.363

5.112 ± 0.289

7.442 ± 0.312

5.468 ± 0.189

5.3 ± 0.306

6.66 ± 0.253

1.524 ± 0.163

2.642 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski