Parainfluenza virus 5 (strain W3) (PIV5) (Simian virus 5)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Orthorubulavirus; Mammalian orthorubulavirus 5

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|Q88435|NCAP_PIV5 Nucleoprotein OS=Parainfluenza virus 5 (strain W3) OX=11208 GN=NP PE=1 SV=1
MM1 pKa = 7.56SSVLKK6 pKa = 10.47AYY8 pKa = 9.85EE9 pKa = 4.19RR10 pKa = 11.84FTLTQEE16 pKa = 4.31LQDD19 pKa = 3.52QSEE22 pKa = 4.75EE23 pKa = 4.22GTIPPTTLKK32 pKa = 10.47PVIRR36 pKa = 11.84VFILTSNNPEE46 pKa = 3.83LRR48 pKa = 11.84SRR50 pKa = 11.84LLLFCLRR57 pKa = 11.84IVLSNGARR65 pKa = 11.84DD66 pKa = 3.57SHH68 pKa = 7.35RR69 pKa = 11.84FGALLTMFSLPSATMLNHH87 pKa = 6.25VKK89 pKa = 10.41LADD92 pKa = 3.6QSPEE96 pKa = 3.53ADD98 pKa = 3.19IEE100 pKa = 4.16RR101 pKa = 11.84VEE103 pKa = 3.83IDD105 pKa = 3.27GFEE108 pKa = 4.04EE109 pKa = 5.24GSFRR113 pKa = 11.84LIPNARR119 pKa = 11.84SGMSRR124 pKa = 11.84GEE126 pKa = 3.88INAYY130 pKa = 9.8AALAEE135 pKa = 4.73DD136 pKa = 5.71LPDD139 pKa = 4.1TLNHH143 pKa = 6.05ATPFVDD149 pKa = 4.9SEE151 pKa = 4.92VEE153 pKa = 3.95GTAWDD158 pKa = 4.18EE159 pKa = 4.14IEE161 pKa = 4.68TFLDD165 pKa = 3.08MCYY168 pKa = 10.57SVLMQAWIVTCKK180 pKa = 10.52CMTAPDD186 pKa = 3.62QPAASIEE193 pKa = 3.98KK194 pKa = 10.06RR195 pKa = 11.84LQKK198 pKa = 10.3YY199 pKa = 8.17RR200 pKa = 11.84QQGRR204 pKa = 11.84INPRR208 pKa = 11.84YY209 pKa = 9.48LLQPEE214 pKa = 4.17ARR216 pKa = 11.84RR217 pKa = 11.84IIQNVIRR224 pKa = 11.84KK225 pKa = 9.26GMVVRR230 pKa = 11.84HH231 pKa = 5.88FLTFEE236 pKa = 4.09LQLARR241 pKa = 11.84AQSLVSNRR249 pKa = 11.84YY250 pKa = 7.77YY251 pKa = 11.74AMVGDD256 pKa = 3.57VGKK259 pKa = 10.91YY260 pKa = 9.56IEE262 pKa = 4.32NCGMGGFFLTLKK274 pKa = 10.07YY275 pKa = 10.78ALGTRR280 pKa = 11.84WPTLALAAFSGEE292 pKa = 4.03LTKK295 pKa = 10.94LKK297 pKa = 10.92SLMALYY303 pKa = 8.09QTLGEE308 pKa = 4.1QARR311 pKa = 11.84YY312 pKa = 8.72LALLEE317 pKa = 4.94SPHH320 pKa = 8.16LMDD323 pKa = 5.63FAAANYY329 pKa = 7.83PLLYY333 pKa = 9.88SYY335 pKa = 11.71AMGIGYY341 pKa = 9.82VLDD344 pKa = 3.66VNMRR348 pKa = 11.84NYY350 pKa = 10.82AFSRR354 pKa = 11.84SYY356 pKa = 10.23MNKK359 pKa = 9.14TYY361 pKa = 10.16FQLGMEE367 pKa = 4.83TARR370 pKa = 11.84KK371 pKa = 7.78QQGAVDD377 pKa = 3.49MRR379 pKa = 11.84MAEE382 pKa = 4.4DD383 pKa = 3.8LGLTQAEE390 pKa = 4.23RR391 pKa = 11.84TEE393 pKa = 4.24MANTLAKK400 pKa = 9.63LTTANRR406 pKa = 11.84GADD409 pKa = 3.22TRR411 pKa = 11.84GGVNPFSSVTGTTQVPAAATGDD433 pKa = 3.78TLEE436 pKa = 5.22SYY438 pKa = 9.57MAADD442 pKa = 4.53RR443 pKa = 11.84LRR445 pKa = 11.84QRR447 pKa = 11.84YY448 pKa = 9.11ADD450 pKa = 4.45AGTHH454 pKa = 6.39DD455 pKa = 5.64DD456 pKa = 4.11EE457 pKa = 4.72MPPLEE462 pKa = 4.75EE463 pKa = 4.59EE464 pKa = 4.57EE465 pKa = 4.82EE466 pKa = 4.78DD467 pKa = 3.84DD468 pKa = 3.74TSAGPRR474 pKa = 11.84TGPTLEE480 pKa = 4.1QVALDD485 pKa = 3.73IQNAAVGAPIHH496 pKa = 6.48TDD498 pKa = 3.42DD499 pKa = 5.38LNAALGDD506 pKa = 3.89LDD508 pKa = 3.57II509 pKa = 6.39

Molecular weight:
56.54 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|Q88434|L_PIV5 RNA-directed RNA polymerase L OS=Parainfluenza virus 5 (strain W3) OX=11208 GN=L PE=1 SV=1
MM1 pKa = 7.68PSISIPADD9 pKa = 3.13PTNPRR14 pKa = 11.84QSIKK18 pKa = 10.76AFPIVINSDD27 pKa = 3.08GGEE30 pKa = 3.91KK31 pKa = 10.56GRR33 pKa = 11.84LVKK36 pKa = 9.9QLRR39 pKa = 11.84TTYY42 pKa = 11.12LNDD45 pKa = 4.07LDD47 pKa = 3.93THH49 pKa = 6.37EE50 pKa = 4.97PLVTFINTYY59 pKa = 10.24GFIYY63 pKa = 10.18EE64 pKa = 3.99QDD66 pKa = 3.28RR67 pKa = 11.84GNTIVGEE74 pKa = 4.08DD75 pKa = 3.34QLGKK79 pKa = 10.38KK80 pKa = 9.83RR81 pKa = 11.84EE82 pKa = 4.16AVTAAMVTLGCGPNLPSLGNVLGQLRR108 pKa = 11.84EE109 pKa = 4.0FQVTVRR115 pKa = 11.84KK116 pKa = 7.95TSSKK120 pKa = 9.45AEE122 pKa = 3.74EE123 pKa = 4.02MVFEE127 pKa = 4.11IVKK130 pKa = 9.73YY131 pKa = 8.79PRR133 pKa = 11.84IFRR136 pKa = 11.84GHH138 pKa = 5.43TLIQKK143 pKa = 10.21GLVCVSAEE151 pKa = 4.2KK152 pKa = 10.22FVKK155 pKa = 10.57SPGKK159 pKa = 9.4IQSGMDD165 pKa = 3.34YY166 pKa = 11.12LFIPTFLSVTYY177 pKa = 9.89CPAAIKK183 pKa = 9.84FQVPGPMLKK192 pKa = 8.94MRR194 pKa = 11.84SRR196 pKa = 11.84YY197 pKa = 6.47TQSLQLEE204 pKa = 4.25LMIRR208 pKa = 11.84ILCKK212 pKa = 9.79PDD214 pKa = 3.31SPLMKK219 pKa = 10.14VHH221 pKa = 6.4TPDD224 pKa = 3.67KK225 pKa = 10.25EE226 pKa = 4.28GRR228 pKa = 11.84GCLVSVWLHH237 pKa = 4.57VCNIFKK243 pKa = 10.67SGNKK247 pKa = 9.49NGSEE251 pKa = 3.74WQEE254 pKa = 3.32YY255 pKa = 7.21WMRR258 pKa = 11.84KK259 pKa = 7.19CANMQLEE266 pKa = 4.3VSIADD271 pKa = 3.19MWGPTIIIHH280 pKa = 6.41ARR282 pKa = 11.84GHH284 pKa = 5.71IPKK287 pKa = 9.82SAKK290 pKa = 10.39LFFGKK295 pKa = 10.44GGWSCHH301 pKa = 5.65PLHH304 pKa = 6.51EE305 pKa = 4.59VVPSVTKK312 pKa = 8.79TLWSVGCEE320 pKa = 3.51ITKK323 pKa = 10.6AKK325 pKa = 10.62AIIQEE330 pKa = 4.25SSISLLVEE338 pKa = 3.81TTDD341 pKa = 3.97IISPKK346 pKa = 10.19VKK348 pKa = 9.79ISSKK352 pKa = 9.77HH353 pKa = 4.19RR354 pKa = 11.84RR355 pKa = 11.84FVKK358 pKa = 10.78SNWGLFKK365 pKa = 10.08KK366 pKa = 9.01TKK368 pKa = 10.1SLPNLTEE375 pKa = 4.03LEE377 pKa = 4.23

Molecular weight:
42.25 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4893

44

2255

611.6

68.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.56 ± 0.995

1.962 ± 0.273

4.66 ± 0.485

4.925 ± 0.655

3.638 ± 0.357

5.702 ± 0.581

1.737 ± 0.392

7.766 ± 0.706

4.558 ± 0.908

11.179 ± 0.767

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.265

5.436 ± 0.477

5.109 ± 0.459

4.312 ± 0.493

4.68 ± 0.477

7.93 ± 0.533

7.46 ± 0.68

5.518 ± 0.731

1.165 ± 0.268

3.25 ± 0.497

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski