Firmicutes bacterium CAG:345

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1462 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6YY12|R6YY12_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:345 OX=1263020 GN=BN617_00872 PE=4 SV=1
MM1 pKa = 7.29GKK3 pKa = 9.56KK4 pKa = 8.76VHH6 pKa = 6.17VNQDD10 pKa = 3.07VCISCGMCTAICDD23 pKa = 3.63AVFAFNDD30 pKa = 3.6DD31 pKa = 3.54GKK33 pKa = 11.19ASVILDD39 pKa = 4.79EE40 pKa = 4.85IPEE43 pKa = 4.3DD44 pKa = 3.8LEE46 pKa = 5.12ASVDD50 pKa = 3.75EE51 pKa = 5.21AKK53 pKa = 10.81NSCPVQAIEE62 pKa = 4.2EE63 pKa = 4.26

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6XQ02|R6XQ02_9FIRM Peptide chain release factor 2 OS=Firmicutes bacterium CAG:345 OX=1263020 GN=prfB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.34QPSKK9 pKa = 10.35RR10 pKa = 11.84KK11 pKa = 8.69KK12 pKa = 8.57QSVNGFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.2VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 5.58KK37 pKa = 10.51GRR39 pKa = 11.84KK40 pKa = 8.42VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1462

0

1462

491748

30

2517

336.4

38.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.241 ± 0.059

1.188 ± 0.021

5.934 ± 0.053

6.749 ± 0.06

5.134 ± 0.051

5.633 ± 0.054

1.497 ± 0.024

9.227 ± 0.075

8.74 ± 0.069

9.29 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.032

6.789 ± 0.074

2.811 ± 0.036

2.424 ± 0.027

2.958 ± 0.041

7.324 ± 0.072

5.366 ± 0.074

5.831 ± 0.055

0.623 ± 0.018

5.128 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski