Mycobacterium sp. 1245111.1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5178 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A3PIS4|A0A1A3PIS4_9MYCO RNA polymerase subunit sigma OS=Mycobacterium sp. 1245111.1 OX=1834073 GN=A5658_15790 PE=3 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84NVTWSSALPRR12 pKa = 11.84RR13 pKa = 11.84MRR15 pKa = 11.84SIGLAATAGAMAMAITAPHH34 pKa = 6.67ANADD38 pKa = 3.88PAYY41 pKa = 10.43QPALDD46 pKa = 3.81QLLEE50 pKa = 4.16AMQQAAAYY58 pKa = 7.73DD59 pKa = 3.62ASLPFATVDD68 pKa = 3.23SDD70 pKa = 3.7AMLMTGLQNTNYY82 pKa = 9.31EE83 pKa = 4.3LLSAGLSRR91 pKa = 11.84VTEE94 pKa = 4.12LFNGVFFQSPEE105 pKa = 3.85AVATDD110 pKa = 3.79AADD113 pKa = 3.21SRR115 pKa = 11.84QYY117 pKa = 11.11FQFFTPDD124 pKa = 2.61IYY126 pKa = 11.44YY127 pKa = 10.32HH128 pKa = 6.0ATAGLAPGATYY139 pKa = 10.82EE140 pKa = 4.14LTGTVGKK147 pKa = 8.72GTEE150 pKa = 3.89ALAIATEE157 pKa = 5.09AITGSTAVSKK167 pKa = 10.95EE168 pKa = 3.98SLEE171 pKa = 4.2LDD173 pKa = 2.91HH174 pKa = 7.05GLVVNPDD181 pKa = 2.9GSFTIDD187 pKa = 3.22IGPTEE192 pKa = 4.05PTGAVNFINDD202 pKa = 3.55TDD204 pKa = 3.55ATTNGDD210 pKa = 3.34ASLLIRR216 pKa = 11.84DD217 pKa = 4.34VLGDD221 pKa = 3.62WAQGPGSISIHH232 pKa = 6.11CVSDD236 pKa = 3.83CPAFFSIPSTGLFPGADD253 pKa = 3.14SSAAEE258 pKa = 4.18IGTLTGTSTPPITSVDD274 pKa = 3.69SLLTTLFTAFSNIVGPFNQEE294 pKa = 3.43NMGLAQVAGIDD305 pKa = 3.97LPPNTMSMLAPEE317 pKa = 4.36TSVFATGLPSADD329 pKa = 3.02VSGGNFDD336 pKa = 5.47LSPDD340 pKa = 3.21QALIVQVPDD349 pKa = 3.44VPSGYY354 pKa = 10.58SGIEE358 pKa = 3.74LMNVFGAALPFTLAQTTLNNTTAFADD384 pKa = 3.73PDD386 pKa = 4.06GYY388 pKa = 9.73TYY390 pKa = 11.32YY391 pKa = 10.81VVSATNPGVANWLDD405 pKa = 3.58SSGVSSGEE413 pKa = 3.3IFARR417 pKa = 11.84FEE419 pKa = 4.01NLPDD423 pKa = 3.98GVDD426 pKa = 3.51PTGLPVTTEE435 pKa = 4.14VVPVADD441 pKa = 3.59VANYY445 pKa = 10.25LPADD449 pKa = 3.98TPTVSPAEE457 pKa = 4.14YY458 pKa = 10.04AADD461 pKa = 3.34MSQRR465 pKa = 11.84VLSYY469 pKa = 10.88DD470 pKa = 3.41YY471 pKa = 11.63ALDD474 pKa = 3.87LSRR477 pKa = 11.84EE478 pKa = 4.08HH479 pKa = 6.93AQPDD483 pKa = 3.42WVLQEE488 pKa = 4.33LLLHH492 pKa = 6.5GFQGLMGTDD501 pKa = 2.72NFAAVFGSEE510 pKa = 3.83PSIPLDD516 pKa = 4.25LRR518 pKa = 11.84FTPALSPDD526 pKa = 3.65WATVDD531 pKa = 4.57HH532 pKa = 7.56DD533 pKa = 4.92LLTNPVASVEE543 pKa = 4.7AIFNTLPLAASDD555 pKa = 3.25ISLPTEE561 pKa = 3.74LAVGQTALSLLLPQQLGSLFNDD583 pKa = 5.4AILDD587 pKa = 3.97PNTGIIAGFLNARR600 pKa = 11.84DD601 pKa = 4.23DD602 pKa = 3.99LATAILTANNGFPTEE617 pKa = 4.16LGSVATAEE625 pKa = 4.18WANMPEE631 pKa = 4.5LVQSTASTLLTQLGALFGAA650 pKa = 4.94

Molecular weight: 67.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A3Q2H2|A0A1A3Q2H2_9MYCO RNA polymerase subunit sigma OS=Mycobacterium sp. 1245111.1 OX=1834073 GN=A5658_02715 PE=3 SV=1
MM1 pKa = 7.32RR2 pKa = 11.84AGRR5 pKa = 11.84VFEE8 pKa = 4.13VTQEE12 pKa = 4.0VLHH15 pKa = 6.0ALIGRR20 pKa = 11.84VTDD23 pKa = 3.03IRR25 pKa = 11.84IRR27 pKa = 11.84LGLWLGLRR35 pKa = 11.84RR36 pKa = 11.84LLLWLGRR43 pKa = 11.84GLLLLRR49 pKa = 11.84WLRR52 pKa = 11.84WSVRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84LRR60 pKa = 11.84SLLGLWRR67 pKa = 11.84RR68 pKa = 11.84AQGLGGVTGAEE79 pKa = 4.08QRR81 pKa = 11.84WLRR84 pKa = 11.84RR85 pKa = 11.84SWRR88 pKa = 11.84GRR90 pKa = 11.84RR91 pKa = 11.84QWVLGRR97 pKa = 11.84HH98 pKa = 4.03IRR100 pKa = 11.84EE101 pKa = 3.95RR102 pKa = 11.84LRR104 pKa = 11.84GNAGGTGQWFRR115 pKa = 11.84HH116 pKa = 4.93

Molecular weight: 13.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5178

0

5178

1648677

27

4134

318.4

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.835 ± 0.049

0.813 ± 0.01

6.426 ± 0.027

5.215 ± 0.033

3.116 ± 0.02

8.736 ± 0.051

2.334 ± 0.016

4.388 ± 0.02

2.321 ± 0.022

9.741 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.066 ± 0.014

2.4 ± 0.018

5.792 ± 0.027

3.113 ± 0.017

7.038 ± 0.04

5.584 ± 0.024

5.924 ± 0.023

8.437 ± 0.031

1.532 ± 0.014

2.188 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski