J-virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Jeilongvirus; Jun jeilongvirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q49HP2|Q49HP2_9MONO Nucleocapsid OS=J-virus OX=322067 GN=N PE=3 SV=1
MM1 pKa = 7.45NSYY4 pKa = 10.47SVQALEE10 pKa = 3.91QLVNDD15 pKa = 5.93GIKK18 pKa = 9.12TAQFFQKK25 pKa = 10.35NQEE28 pKa = 4.49NIQKK32 pKa = 9.13TYY34 pKa = 9.93GRR36 pKa = 11.84SAIGLPTTKK45 pKa = 10.2EE46 pKa = 3.95RR47 pKa = 11.84ISAWEE52 pKa = 3.84AVAEE56 pKa = 4.36TPYY59 pKa = 11.17GEE61 pKa = 4.13QIQLGGGNGEE71 pKa = 4.21DD72 pKa = 3.58QRR74 pKa = 11.84GEE76 pKa = 3.95QAEE79 pKa = 4.44GQDD82 pKa = 3.52NPGGHH87 pKa = 5.27GHH89 pKa = 6.65GGEE92 pKa = 4.64EE93 pKa = 4.48IPTGANPSALQLQGSYY109 pKa = 9.95NQVWDD114 pKa = 4.28GPNVATYY121 pKa = 10.71SGGEE125 pKa = 4.11GGDD128 pKa = 3.33TGNQHH133 pKa = 6.03GRR135 pKa = 11.84TDD137 pKa = 3.44SGSAADD143 pKa = 3.96TGGSNQSDD151 pKa = 3.82GSSTYY156 pKa = 9.67STVDD160 pKa = 3.02AGDD163 pKa = 3.85LRR165 pKa = 11.84QMMIFDD171 pKa = 4.86HH172 pKa = 6.04EE173 pKa = 4.35TSAIEE178 pKa = 4.36VGASKK183 pKa = 11.06NNTMKK188 pKa = 10.16IRR190 pKa = 11.84NATEE194 pKa = 3.58EE195 pKa = 4.02DD196 pKa = 3.5LGNVMSEE203 pKa = 4.29GTSKK207 pKa = 9.56IHH209 pKa = 6.15KK210 pKa = 9.2RR211 pKa = 11.84LRR213 pKa = 11.84GVAAMTDD220 pKa = 3.92PLPVPRR226 pKa = 11.84TTGGPVKK233 pKa = 10.46KK234 pKa = 10.31GIEE237 pKa = 4.11EE238 pKa = 4.42SSVSTILGGRR248 pKa = 11.84PTSGSGAIPNVHH260 pKa = 7.01PSLLLLPSQDD270 pKa = 2.88VHH272 pKa = 8.56AEE274 pKa = 4.05SAPKK278 pKa = 10.19CVQDD282 pKa = 3.5VSKK285 pKa = 9.74TGSTCQSSKK294 pKa = 11.38AEE296 pKa = 3.96QDD298 pKa = 3.3CSGIEE303 pKa = 4.03GKK305 pKa = 9.8IDD307 pKa = 3.82LLLGNLEE314 pKa = 4.23KK315 pKa = 10.28VTRR318 pKa = 11.84ALDD321 pKa = 3.38VLPEE325 pKa = 3.59IRR327 pKa = 11.84EE328 pKa = 4.05EE329 pKa = 3.63IRR331 pKa = 11.84NINKK335 pKa = 10.34KK336 pKa = 8.04ITNLSLGLSTVEE348 pKa = 4.54GYY350 pKa = 9.82IKK352 pKa = 10.95SMMIIIPGSGKK363 pKa = 9.55PNPGDD368 pKa = 3.57TPEE371 pKa = 4.17VNPDD375 pKa = 3.28LKK377 pKa = 11.17AVIGRR382 pKa = 11.84DD383 pKa = 3.25NTRR386 pKa = 11.84GLSEE390 pKa = 4.21VRR392 pKa = 11.84SQKK395 pKa = 10.38INLEE399 pKa = 4.14SLTDD403 pKa = 4.09DD404 pKa = 4.36PPSLGTIDD412 pKa = 3.72EE413 pKa = 4.35SHH415 pKa = 6.93IVRR418 pKa = 11.84DD419 pKa = 4.02LDD421 pKa = 3.83FTKK424 pKa = 11.05SNAANFAPTNRR435 pKa = 11.84FSSVMTMFNMIKK447 pKa = 10.45EE448 pKa = 4.2EE449 pKa = 4.04VKK451 pKa = 10.5DD452 pKa = 3.75YY453 pKa = 11.6NMRR456 pKa = 11.84AGLTRR461 pKa = 11.84WLEE464 pKa = 3.97DD465 pKa = 4.0AIEE468 pKa = 4.54RR469 pKa = 11.84LDD471 pKa = 3.92ADD473 pKa = 3.72DD474 pKa = 3.99VYY476 pKa = 11.11RR477 pKa = 11.84ALRR480 pKa = 11.84RR481 pKa = 11.84TLDD484 pKa = 3.25RR485 pKa = 11.84LAEE488 pKa = 4.5DD489 pKa = 5.09DD490 pKa = 4.53EE491 pKa = 6.64DD492 pKa = 6.11DD493 pKa = 4.86EE494 pKa = 6.0EE495 pKa = 4.62EE496 pKa = 5.09DD497 pKa = 3.87EE498 pKa = 4.33NN499 pKa = 4.99

Molecular weight: 53.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q49HN5|Q49HN5_9MONO Small hydrophobic protein OS=J-virus OX=322067 GN=SH PE=4 SV=1
MM1 pKa = 7.49TSTVYY6 pKa = 10.64EE7 pKa = 4.46DD8 pKa = 4.08PEE10 pKa = 4.54SALSGYY16 pKa = 10.86EE17 pKa = 3.94SMRR20 pKa = 11.84SDD22 pKa = 3.96GARR25 pKa = 11.84GLPALPRR32 pKa = 11.84RR33 pKa = 11.84CKK35 pKa = 9.21TANTYY40 pKa = 10.42RR41 pKa = 11.84SARR44 pKa = 11.84RR45 pKa = 11.84VRR47 pKa = 11.84VVTRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84VNNSVYY59 pKa = 9.72FVFIIVCLAILVAMAAYY76 pKa = 9.66VIIGIEE82 pKa = 3.96GLKK85 pKa = 7.47YY86 pKa = 9.22TRR88 pKa = 11.84YY89 pKa = 9.46NSKK92 pKa = 10.83DD93 pKa = 3.4VGPDD97 pKa = 3.05GSKK100 pKa = 10.43RR101 pKa = 11.84LEE103 pKa = 5.56DD104 pKa = 3.7IDD106 pKa = 4.34QKK108 pKa = 11.49LNQISSAVNTIMNALTYY125 pKa = 10.01SVPSVLSTYY134 pKa = 8.42RR135 pKa = 11.84TSLLNRR141 pKa = 11.84INHH144 pKa = 7.09LATEE148 pKa = 4.5LKK150 pKa = 10.26EE151 pKa = 3.9AARR154 pKa = 11.84MNNVDD159 pKa = 5.94LDD161 pKa = 4.44VKK163 pKa = 9.77WGSNRR168 pKa = 11.84TVLLKK173 pKa = 10.05TGSRR177 pKa = 11.84FHH179 pKa = 6.2QLNTRR184 pKa = 11.84EE185 pKa = 4.26LTTKK189 pKa = 8.88NTLVTSYY196 pKa = 10.7PRR198 pKa = 11.84MPTIIPKK205 pKa = 9.63VDD207 pKa = 3.16KK208 pKa = 10.23KK209 pKa = 10.83PPSFYY214 pKa = 10.47PLMKK218 pKa = 10.09VDD220 pKa = 4.9SDD222 pKa = 3.25QDD224 pKa = 3.68LNEE227 pKa = 4.11KK228 pKa = 9.79VKK230 pKa = 10.84AVTKK234 pKa = 9.97IFHH237 pKa = 6.85DD238 pKa = 3.54MSVTKK243 pKa = 10.44DD244 pKa = 3.67SQDD247 pKa = 3.23EE248 pKa = 4.72AIWNLNPASKK258 pKa = 10.76

Molecular weight: 29.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

3

11

5899

69

2204

536.3

60.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.628 ± 0.643

1.848 ± 0.303

5.509 ± 0.305

5.815 ± 0.458

3.357 ± 0.512

6.628 ± 1.178

2.0 ± 0.329

6.967 ± 0.515

5.882 ± 0.505

9.171 ± 0.807

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.068 ± 0.224

5.696 ± 0.29

4.357 ± 0.345

3.56 ± 0.498

5.035 ± 0.452

8.069 ± 0.417

6.306 ± 0.436

6.12 ± 0.387

1.17 ± 0.13

3.814 ± 0.479
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski