Pseudomonas pohangensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2HUI6|A0A1H2HUI6_9PSED Glucose-6-phosphate 1-dehydrogenase OS=Pseudomonas pohangensis OX=364197 GN=zwf PE=3 SV=1
MM1 pKa = 7.53NKK3 pKa = 8.88TNNATPFAKK12 pKa = 9.81TPLVIAIACAGWMSAAQAGEE32 pKa = 4.05ILDD35 pKa = 4.19LSYY38 pKa = 8.3TTGDD42 pKa = 3.49GYY44 pKa = 10.86ILIDD48 pKa = 3.8ADD50 pKa = 4.14EE51 pKa = 4.45EE52 pKa = 4.78VVAPGVKK59 pKa = 10.07AVTTDD64 pKa = 3.41LNNNDD69 pKa = 3.24FVGNGFSPSGDD80 pKa = 4.1PNCLMASSPATCDD93 pKa = 3.21SPKK96 pKa = 10.83NSGKK100 pKa = 10.4RR101 pKa = 11.84IKK103 pKa = 10.97NNLTGRR109 pKa = 11.84GAFEE113 pKa = 3.58HH114 pKa = 6.5VYY116 pKa = 10.31NVKK119 pKa = 9.82TSGGTTEE126 pKa = 3.81YY127 pKa = 11.09LNYY130 pKa = 10.91GKK132 pKa = 8.43ITNRR136 pKa = 11.84TGARR140 pKa = 11.84LLGYY144 pKa = 8.39QVIVGTGTGANFTPASASDD163 pKa = 3.69QAGLLAMDD171 pKa = 4.2NVLPLSGQATSWPGTTQAEE190 pKa = 4.69GQSPLQRR197 pKa = 11.84TWLPEE202 pKa = 3.71GLFGTKK208 pKa = 8.37QDD210 pKa = 3.3MSAGYY215 pKa = 9.22FSPDD219 pKa = 2.69NAGFFFEE226 pKa = 4.75TVGTDD231 pKa = 3.22TLSADD236 pKa = 3.23GMFANTVYY244 pKa = 10.89SQFFGDD250 pKa = 3.91GLLTRR255 pKa = 11.84GQQPQALFLDD265 pKa = 4.0NLDD268 pKa = 4.55PDD270 pKa = 3.94VEE272 pKa = 4.35DD273 pKa = 6.49DD274 pKa = 3.33IVYY277 pKa = 9.72WKK279 pKa = 10.79AGNLWLDD286 pKa = 3.4AAGTVQDD293 pKa = 4.21TATVDD298 pKa = 3.4ALLATPGYY306 pKa = 10.12YY307 pKa = 10.13VDD309 pKa = 4.56TIEE312 pKa = 6.36DD313 pKa = 3.79LTNLNINFSMDD324 pKa = 3.14IGDD327 pKa = 3.88LTAGQFTVRR336 pKa = 11.84YY337 pKa = 8.79VPVFSPIVNAASSDD351 pKa = 3.82YY352 pKa = 10.74QLAVANSLDD361 pKa = 3.68ATQIPYY367 pKa = 10.85LFFDD371 pKa = 3.62QNTAAGATPTPTAAYY386 pKa = 10.71YY387 pKa = 9.65EE388 pKa = 4.45FQDD391 pKa = 4.33IMNAFDD397 pKa = 4.72ALDD400 pKa = 3.95PAAQSQALGSMGTSYY415 pKa = 10.95LRR417 pKa = 11.84NYY419 pKa = 7.31GTQGLLMGRR428 pKa = 11.84GTLEE432 pKa = 3.82AVQQHH437 pKa = 5.21LQEE440 pKa = 4.33NRR442 pKa = 11.84ISSLTPSSVSAEE454 pKa = 3.98TLANQLMGTPGAATLDD470 pKa = 3.69QVVGQPITSTADD482 pKa = 3.09LAMALDD488 pKa = 4.64DD489 pKa = 4.59NGNTASVALNEE500 pKa = 4.34TTSTFISGSASEE512 pKa = 4.83GNLDD516 pKa = 3.68NSNNGTGADD525 pKa = 3.61YY526 pKa = 10.87SAYY529 pKa = 10.35SLTVGLDD536 pKa = 2.92HH537 pKa = 7.11YY538 pKa = 11.38LRR540 pKa = 11.84DD541 pKa = 3.75QLRR544 pKa = 11.84VGAALGYY551 pKa = 9.32GHH553 pKa = 6.85NEE555 pKa = 3.44GDD557 pKa = 4.05VNDD560 pKa = 4.0NYY562 pKa = 11.68GNLDD566 pKa = 3.65LDD568 pKa = 4.77GYY570 pKa = 11.12SLTTFVSYY578 pKa = 11.01GGATGLFTDD587 pKa = 4.17VLLGYY592 pKa = 10.17SWLDD596 pKa = 3.18YY597 pKa = 11.65DD598 pKa = 4.6NDD600 pKa = 3.65RR601 pKa = 11.84KK602 pKa = 10.64INIGSEE608 pKa = 3.76QRR610 pKa = 11.84KK611 pKa = 9.47AKK613 pKa = 10.81SNTDD617 pKa = 2.88GDD619 pKa = 4.1LSSFAVRR626 pKa = 11.84SGYY629 pKa = 10.06NFEE632 pKa = 5.15LGPVIAGPSLRR643 pKa = 11.84YY644 pKa = 9.62QYY646 pKa = 11.49LDD648 pKa = 4.1LSVDD652 pKa = 4.04DD653 pKa = 4.35YY654 pKa = 10.89TEE656 pKa = 4.1KK657 pKa = 10.97DD658 pKa = 3.17AGVLNMHH665 pKa = 6.46VDD667 pKa = 3.49DD668 pKa = 4.66MSYY671 pKa = 11.35DD672 pKa = 3.45SSTLGLGGQMTMPIALEE689 pKa = 4.15SGFLRR694 pKa = 11.84PYY696 pKa = 11.27AEE698 pKa = 3.96AHH700 pKa = 5.08WVKK703 pKa = 10.37EE704 pKa = 4.03FQDD707 pKa = 4.63DD708 pKa = 3.72SSQVDD713 pKa = 3.37TSFVSGVVPFQTPIDD728 pKa = 4.52AYY730 pKa = 10.59DD731 pKa = 3.57SDD733 pKa = 4.52YY734 pKa = 8.73MTVGAGVEE742 pKa = 4.41TTFMASGMPASLSVNYY758 pKa = 10.35DD759 pKa = 3.32GVVSNSDD766 pKa = 3.29YY767 pKa = 11.16SDD769 pKa = 3.28NRR771 pKa = 11.84VSLDD775 pKa = 3.28LRR777 pKa = 11.84LAFF780 pKa = 5.09

Molecular weight: 82.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2HY28|A0A1H2HY28_9PSED Ribosomal RNA small subunit methyltransferase G OS=Pseudomonas pohangensis OX=364197 GN=rsmG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.24NGRR28 pKa = 11.84LVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3431

0

3431

1117736

29

4753

325.8

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.014 ± 0.048

1.103 ± 0.014

5.227 ± 0.039

5.744 ± 0.045

3.683 ± 0.027

7.85 ± 0.044

2.197 ± 0.02

4.891 ± 0.03

3.554 ± 0.036

12.123 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.019

3.055 ± 0.027

4.862 ± 0.029

4.853 ± 0.037

6.284 ± 0.047

5.821 ± 0.031

4.567 ± 0.056

6.751 ± 0.035

1.456 ± 0.018

2.559 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski