Pseudokineococcus lusitanus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Kineosporiales; Kineosporiaceae; Pseudokineococcus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1G9Z6|A0A3N1G9Z6_9ACTN MsuE subfamily FMN reductase OS=Pseudokineococcus lusitanus OX=763993 GN=EDC03_2990 PE=4 SV=1
MM1 pKa = 7.33TRR3 pKa = 11.84TALTSTPRR11 pKa = 11.84RR12 pKa = 11.84THH14 pKa = 5.53ARR16 pKa = 11.84RR17 pKa = 11.84AASAVVALTTAAALAACGGGSDD39 pKa = 4.34GGNAGGDD46 pKa = 3.9EE47 pKa = 4.16NTLTWWHH54 pKa = 6.39NSNTDD59 pKa = 3.26PGAGYY64 pKa = 10.26YY65 pKa = 10.37EE66 pKa = 4.13EE67 pKa = 4.1VAAAFEE73 pKa = 4.25EE74 pKa = 4.68ANPGTTIEE82 pKa = 4.27ISALPHH88 pKa = 6.21EE89 pKa = 5.45DD90 pKa = 2.74MLTRR94 pKa = 11.84LNAAFQSGDD103 pKa = 3.43VPDD106 pKa = 4.19VYY108 pKa = 10.08MEE110 pKa = 4.22RR111 pKa = 11.84GGGEE115 pKa = 3.91LADD118 pKa = 3.74HH119 pKa = 6.42VEE121 pKa = 4.54AGLTMDD127 pKa = 4.74LSEE130 pKa = 4.72GAADD134 pKa = 4.08TIEE137 pKa = 4.66KK138 pKa = 10.23IGGSVAGWQVDD149 pKa = 3.47GATYY153 pKa = 10.84ALPFSVGVVGFWYY166 pKa = 8.55NTEE169 pKa = 4.18LFEE172 pKa = 4.79QAGITEE178 pKa = 4.87APTTMEE184 pKa = 4.26EE185 pKa = 4.16LDD187 pKa = 3.94AAVQALKK194 pKa = 10.76DD195 pKa = 3.7AGIQPISVGAGDD207 pKa = 3.99TWPAAHH213 pKa = 6.13YY214 pKa = 8.78WYY216 pKa = 10.53YY217 pKa = 10.88LAVRR221 pKa = 11.84EE222 pKa = 4.44CGQDD226 pKa = 3.36VLTDD230 pKa = 3.7AVTSLDD236 pKa = 3.74FSDD239 pKa = 3.69EE240 pKa = 4.3CFVRR244 pKa = 11.84AGEE247 pKa = 4.22DD248 pKa = 3.19LEE250 pKa = 5.37AFIGTEE256 pKa = 3.79PFNEE260 pKa = 4.4GFLATPAQVGPASASGLLASEE281 pKa = 4.14QVAMEE286 pKa = 4.54LAGHH290 pKa = 6.32WEE292 pKa = 4.01PGVMQGLTEE301 pKa = 4.36GGEE304 pKa = 4.34GLGEE308 pKa = 3.99KK309 pKa = 9.37TGWFAFPTVEE319 pKa = 4.3GGEE322 pKa = 4.35GAPDD326 pKa = 3.45AALGGGDD333 pKa = 3.7AWACAADD340 pKa = 4.12APPVCVDD347 pKa = 3.62FVNYY351 pKa = 10.22LLSDD355 pKa = 4.07EE356 pKa = 4.52VQTGFAEE363 pKa = 4.26RR364 pKa = 11.84DD365 pKa = 3.13MGLPTNPAATGAVSDD380 pKa = 4.55PALADD385 pKa = 4.62LIAVRR390 pKa = 11.84DD391 pKa = 3.82SAPYY395 pKa = 8.02VQLYY399 pKa = 10.15FDD401 pKa = 3.63TAFGEE406 pKa = 4.49NVGGAMNDD414 pKa = 3.81AIALMFAGQGSAQDD428 pKa = 3.76VVDD431 pKa = 4.22ATQSAAGTAA440 pKa = 3.53

Molecular weight: 45.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1HMB5|A0A3N1HMB5_9ACTN tRNA pseudouridine synthase B OS=Pseudokineococcus lusitanus OX=763993 GN=truB PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.64HH17 pKa = 5.77RR18 pKa = 11.84KK19 pKa = 7.85LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84HH26 pKa = 3.6QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3389

0

3389

1148759

29

3317

339.0

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.619 ± 0.072

0.567 ± 0.009

6.622 ± 0.04

5.314 ± 0.038

2.003 ± 0.022

10.391 ± 0.041

1.906 ± 0.022

1.532 ± 0.027

1.01 ± 0.018

10.746 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.312 ± 0.015

1.048 ± 0.018

6.592 ± 0.036

2.301 ± 0.022

8.509 ± 0.05

4.457 ± 0.028

6.004 ± 0.046

11.154 ± 0.045

1.39 ± 0.016

1.524 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski