Apis mellifera associated microvirus 42

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UU94|A0A3S8UU94_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 42 OX=2494772 PE=4 SV=1
MM1 pKa = 7.53AKK3 pKa = 10.04AASSQKK9 pKa = 10.02HH10 pKa = 5.15EE11 pKa = 4.46EE12 pKa = 4.34FIQPSAADD20 pKa = 3.69TYY22 pKa = 11.0LDD24 pKa = 3.49SKK26 pKa = 11.17GRR28 pKa = 11.84EE29 pKa = 4.27KK30 pKa = 10.72PDD32 pKa = 3.45PTPIAPPIGYY42 pKa = 9.18KK43 pKa = 9.93RR44 pKa = 11.84QPSLAEE50 pKa = 3.75QIRR53 pKa = 11.84NMVRR57 pKa = 11.84SEE59 pKa = 4.07RR60 pKa = 11.84LAMEE64 pKa = 4.72AAAAGYY70 pKa = 7.82GTFEE74 pKa = 4.63EE75 pKa = 5.1EE76 pKa = 4.83DD77 pKa = 3.84DD78 pKa = 4.11FDD80 pKa = 3.95TGEE83 pKa = 4.5DD84 pKa = 3.69LDD86 pKa = 4.11PRR88 pKa = 11.84SPYY91 pKa = 10.51EE92 pKa = 3.88IDD94 pKa = 4.25FDD96 pKa = 4.89PPLQTISPPQQEE108 pKa = 5.0GGSSPAVPPPVDD120 pKa = 3.58TPSPNPSAA128 pKa = 4.39

Molecular weight: 13.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UUA8|A0A3S8UUA8_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 42 OX=2494772 PE=4 SV=1
MM1 pKa = 7.5AKK3 pKa = 9.91SRR5 pKa = 11.84NRR7 pKa = 11.84TGRR10 pKa = 11.84RR11 pKa = 11.84GNNTTQTTAIRR22 pKa = 11.84TRR24 pKa = 11.84MPLPTVALVTLSHH37 pKa = 5.57TTYY40 pKa = 10.12PDD42 pKa = 2.98RR43 pKa = 11.84RR44 pKa = 11.84TWSPARR50 pKa = 11.84VLSGPAARR58 pKa = 11.84NVNATRR64 pKa = 11.84LVAADD69 pKa = 3.7RR70 pKa = 11.84VGDD73 pKa = 3.67AVRR76 pKa = 11.84RR77 pKa = 11.84QTKK80 pKa = 9.91APIRR84 pKa = 11.84FAVPEE89 pKa = 4.07KK90 pKa = 10.63VALCVRR96 pKa = 11.84RR97 pKa = 11.84QRR99 pKa = 11.84RR100 pKa = 11.84KK101 pKa = 9.54EE102 pKa = 3.68VLHH105 pKa = 6.19AFKK108 pKa = 10.78KK109 pKa = 10.43AGRR112 pKa = 11.84KK113 pKa = 9.15GPQKK117 pKa = 10.06KK118 pKa = 8.47HH119 pKa = 6.38HH120 pKa = 6.59RR121 pKa = 11.84NQWSNVSCKK130 pKa = 9.72RR131 pKa = 3.4

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1318

128

490

263.6

29.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.105 ± 1.105

0.986 ± 0.55

4.097 ± 0.599

5.842 ± 0.706

3.414 ± 0.42

8.574 ± 0.842

2.124 ± 0.425

4.552 ± 0.735

4.704 ± 1.207

7.663 ± 0.918

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.973 ± 0.114

3.945 ± 0.488

6.904 ± 1.12

4.856 ± 0.615

7.815 ± 1.214

6.07 ± 0.654

6.601 ± 0.838

5.994 ± 0.815

1.442 ± 0.241

3.338 ± 0.558
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski