Bosea sp. BK498

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6GWK9|A0A4R6GWK9_9BRAD Uncharacterized protein OS=Bosea sp. BK498 OX=2512178 GN=EV277_3081 PE=4 SV=1
MM1 pKa = 7.75AYY3 pKa = 10.06AVTEE7 pKa = 3.74ACIRR11 pKa = 11.84CKK13 pKa = 10.31YY14 pKa = 7.53TDD16 pKa = 4.02CVAVCPVDD24 pKa = 3.67CFYY27 pKa = 11.22EE28 pKa = 4.96GEE30 pKa = 4.34TMLVINPDD38 pKa = 3.09EE39 pKa = 5.49CIDD42 pKa = 4.08CGVCEE47 pKa = 4.44PEE49 pKa = 4.88CPAEE53 pKa = 4.92AIIPDD58 pKa = 3.93TAEE61 pKa = 4.28GSARR65 pKa = 11.84WVALNGRR72 pKa = 11.84LAAQWPGITDD82 pKa = 3.41KK83 pKa = 11.22RR84 pKa = 11.84DD85 pKa = 3.32APADD89 pKa = 3.66AEE91 pKa = 4.22DD92 pKa = 3.54WKK94 pKa = 11.29GIPDD98 pKa = 3.82KK99 pKa = 11.34LQFLPEE105 pKa = 4.31ALDD108 pKa = 3.48AA109 pKa = 5.55

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V3BYG0|A0A4V3BYG0_9BRAD UDP-N-acetylglucosamine 1-carboxyvinyltransferase OS=Bosea sp. BK498 OX=2512178 GN=murA PE=3 SV=1
MM1 pKa = 7.29SPKK4 pKa = 9.66IRR6 pKa = 11.84PAGSLSLAIKK16 pKa = 10.46LVMLAMVVPRR26 pKa = 11.84VVRR29 pKa = 11.84FKK31 pKa = 10.97LRR33 pKa = 11.84RR34 pKa = 11.84GRR36 pKa = 3.68

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5154

0

5154

1633446

29

2557

316.9

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.388 ± 0.045

0.78 ± 0.01

5.274 ± 0.025

5.562 ± 0.032

3.709 ± 0.019

8.885 ± 0.039

1.896 ± 0.016

5.14 ± 0.023

3.23 ± 0.028

10.539 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.348 ± 0.014

2.342 ± 0.022

5.466 ± 0.028

3.043 ± 0.018

7.274 ± 0.034

5.24 ± 0.024

5.118 ± 0.026

7.374 ± 0.027

1.311 ± 0.013

2.078 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski