Pseudomonas stutzeri (strain A1501)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas stutzeri group; Pseudomonas stutzeri subgroup; Pseudomonas stutzeri

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4092 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4VJF2|A4VJF2_PSEU5 EAL domain/GGDEF domain protein OS=Pseudomonas stutzeri (strain A1501) OX=379731 GN=PST_1412 PE=4 SV=1
MM1 pKa = 7.09TFNVALSGLNAASTDD16 pKa = 3.99LNVTGNNIANVGTTGFKK33 pKa = 10.45ASRR36 pKa = 11.84AEE38 pKa = 4.01FADD41 pKa = 4.85LYY43 pKa = 8.49ATSMLGSGGNAVGSGVTTADD63 pKa = 2.89IAQQFTQGNITNTGNSLDD81 pKa = 4.17LAISGNGYY89 pKa = 10.8FMVSNNGSMLYY100 pKa = 9.49TRR102 pKa = 11.84AGAFKK107 pKa = 9.96TDD109 pKa = 3.43DD110 pKa = 3.51EE111 pKa = 5.47GYY113 pKa = 10.01VINSEE118 pKa = 4.39GYY120 pKa = 7.83NLQGYY125 pKa = 7.67GVNANGDD132 pKa = 3.56IVSGVTTNLKK142 pKa = 10.09VDD144 pKa = 4.21SSNQAPNATSSVVEE158 pKa = 4.03TLALNSNSSVPNNTPFDD175 pKa = 4.13ASDD178 pKa = 3.05SSTYY182 pKa = 10.36NWSTSVPLYY191 pKa = 8.57DD192 pKa = 3.63TQGNSHH198 pKa = 6.49TMTQYY203 pKa = 10.42FVKK206 pKa = 10.34DD207 pKa = 4.65DD208 pKa = 3.92SNQWTMYY215 pKa = 9.77TLIDD219 pKa = 3.85GRR221 pKa = 11.84NPADD225 pKa = 3.56PTSTEE230 pKa = 3.91AYY232 pKa = 7.85EE233 pKa = 3.99VSIGFTASGALDD245 pKa = 3.87LTSLASTDD253 pKa = 3.78FDD255 pKa = 4.41VNADD259 pKa = 3.47GTLTLNTWVPATVTDD274 pKa = 4.11STTSPVTWGSNGAAASASGISIDD297 pKa = 5.08LRR299 pKa = 11.84NISQTNTSFAVLSSPVQDD317 pKa = 4.58GYY319 pKa = 8.25TTGQLSGLSIDD330 pKa = 3.85DD331 pKa = 3.69TGMMFATYY339 pKa = 10.28TNGQSKK345 pKa = 10.98VIGQVVLANFTNSQGLASVGGTAWAQTLASGEE377 pKa = 4.15PAIGTPGSGTLGSLQSGALEE397 pKa = 4.53DD398 pKa = 4.34SNVDD402 pKa = 3.41LTAQLVNLIVAQRR415 pKa = 11.84NYY417 pKa = 9.52QANAKK422 pKa = 7.98TVEE425 pKa = 4.3TEE427 pKa = 3.85SAIAQTIINLRR438 pKa = 3.68

Molecular weight: 45.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4VFG7|A4VFG7_PSEU5 Glycine--tRNA ligase alpha subunit OS=Pseudomonas stutzeri (strain A1501) OX=379731 GN=glyQ PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4092

0

4092

1346991

31

2444

329.2

36.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.315 ± 0.043

1.046 ± 0.013

5.345 ± 0.031

6.131 ± 0.034

3.564 ± 0.023

7.979 ± 0.034

2.305 ± 0.018

4.645 ± 0.028

3.089 ± 0.032

12.01 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.017

2.744 ± 0.023

4.944 ± 0.027

4.545 ± 0.03

7.234 ± 0.033

5.484 ± 0.026

4.532 ± 0.023

6.895 ± 0.031

1.428 ± 0.016

2.45 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski