Bdellovibrio bacteriovorus W

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Bdellovibrionales; Bdellovibrionaceae; Bdellovibrio; Bdellovibrio bacteriovorus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2854 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5WW32|W5WW32_BDEBC S-adenosylmethionine synthase OS=Bdellovibrio bacteriovorus W OX=765869 GN=metK PE=3 SV=1
MM1 pKa = 7.65AEE3 pKa = 5.46KK4 pKa = 10.01GQKK7 pKa = 8.44WNEE10 pKa = 3.66NVAGKK15 pKa = 9.6MYY17 pKa = 10.82VDD19 pKa = 4.2QSCIACDD26 pKa = 3.66ACILTAPNNFAMHH39 pKa = 7.33DD40 pKa = 3.88EE41 pKa = 4.87DD42 pKa = 3.58GHH44 pKa = 7.96AFVVKK49 pKa = 10.41QPSTPEE55 pKa = 3.89EE56 pKa = 4.06EE57 pKa = 4.55EE58 pKa = 4.34LCQEE62 pKa = 4.53AMDD65 pKa = 4.45GCPVEE70 pKa = 5.77AIGSDD75 pKa = 3.5GDD77 pKa = 3.51QQ78 pKa = 3.29

Molecular weight: 8.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5WXI2|W5WXI2_BDEBC S-adenosyl-L-homocysteine hydrolase OS=Bdellovibrio bacteriovorus W OX=765869 GN=BDW_04615 PE=3 SV=1
MM1 pKa = 7.31AVKK4 pKa = 10.32GKK6 pKa = 9.02SKK8 pKa = 10.55KK9 pKa = 10.3GIRR12 pKa = 11.84HH13 pKa = 4.79KK14 pKa = 10.14MKK16 pKa = 10.62RR17 pKa = 11.84IAKK20 pKa = 7.81KK21 pKa = 10.22KK22 pKa = 9.82RR23 pKa = 11.84MIRR26 pKa = 11.84VKK28 pKa = 10.61KK29 pKa = 10.3RR30 pKa = 2.95

Molecular weight: 3.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2854

0

2854

902569

30

2884

316.2

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.884 ± 0.047

0.895 ± 0.016

5.056 ± 0.027

6.685 ± 0.048

4.735 ± 0.039

6.825 ± 0.046

1.935 ± 0.022

6.22 ± 0.038

6.789 ± 0.047

9.946 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.026

4.302 ± 0.034

4.02 ± 0.029

3.966 ± 0.033

4.707 ± 0.036

7.425 ± 0.045

5.185 ± 0.043

6.746 ± 0.037

1.148 ± 0.019

3.017 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski