Parasaccharibacter apium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Parasaccharibacter

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1608 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C6J4V6|A0A2C6J4V6_9PROT Porphobilinogen deaminase OS=Parasaccharibacter apium OX=1510841 GN=hemC PE=3 SV=1
MM1 pKa = 7.37TYY3 pKa = 10.66VVTEE7 pKa = 3.68NCVRR11 pKa = 11.84CKK13 pKa = 10.75FMDD16 pKa = 4.05CVEE19 pKa = 4.27VCPVDD24 pKa = 3.86CFYY27 pKa = 11.41AGEE30 pKa = 4.18NFLVINPDD38 pKa = 2.99EE39 pKa = 5.52CIDD42 pKa = 4.08CGVCEE47 pKa = 4.44PEE49 pKa = 4.91CPAEE53 pKa = 4.95AIFPDD58 pKa = 3.67SDD60 pKa = 3.38EE61 pKa = 5.52RR62 pKa = 11.84SADD65 pKa = 3.0WMEE68 pKa = 4.07INAKK72 pKa = 10.22YY73 pKa = 10.74SSIWPNITQKK83 pKa = 9.46GTPPADD89 pKa = 3.31CEE91 pKa = 3.98EE92 pKa = 4.44WNGKK96 pKa = 7.1EE97 pKa = 4.61GKK99 pKa = 9.81RR100 pKa = 11.84EE101 pKa = 3.84MLSEE105 pKa = 4.45EE106 pKa = 4.11PHH108 pKa = 6.59KK109 pKa = 11.04DD110 pKa = 3.02

Molecular weight: 12.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C6J560|A0A2C6J560_9PROT Phosphoribosylglycinamide formyltransferase OS=Parasaccharibacter apium OX=1510841 GN=purN PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.11RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.5GFRR19 pKa = 11.84TRR21 pKa = 11.84SATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 8.82KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1608

0

1608

544650

41

2152

338.7

37.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.842 ± 0.068

1.041 ± 0.02

5.002 ± 0.042

5.745 ± 0.069

3.535 ± 0.038

8.214 ± 0.071

2.968 ± 0.039

5.339 ± 0.05

3.449 ± 0.047

10.831 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.029

2.78 ± 0.048

5.395 ± 0.055

4.111 ± 0.044

6.584 ± 0.061

5.682 ± 0.051

5.609 ± 0.051

6.581 ± 0.056

1.307 ± 0.024

2.388 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski