Rikenella microfusus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia;

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2358 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A379MP92|A0A379MP92_9BACT Cd1 OS=Rikenella microfusus OX=28139 GN=NCTC11190_00753 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.49KK3 pKa = 10.28LFQFVCVAAIAAGFAACGGNAANTEE28 pKa = 4.33NADD31 pKa = 3.42STAVVEE37 pKa = 4.25MSEE40 pKa = 4.41TVVVDD45 pKa = 4.3TVAVDD50 pKa = 3.64TTACDD55 pKa = 3.44TTACDD60 pKa = 3.28SAAVAKK66 pKa = 10.58

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A379MWB1|A0A379MWB1_9BACT Uncharacterized protein OS=Rikenella microfusus OX=28139 GN=NCTC11190_02383 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 3.88KK3 pKa = 11.02HH4 pKa = 6.2PFLRR8 pKa = 11.84NFTNKK13 pKa = 9.33IPKK16 pKa = 9.97LLFLLAVLYY25 pKa = 10.65ALSQNKK31 pKa = 9.32GMLFGHH37 pKa = 6.26DD38 pKa = 4.03TEE40 pKa = 4.72PVRR43 pKa = 11.84ADD45 pKa = 3.29TPTPFDD51 pKa = 3.75TTCANAFFKK60 pKa = 10.89RR61 pKa = 11.84PVVLSTQDD69 pKa = 3.08SVLYY73 pKa = 9.37EE74 pKa = 3.98VRR76 pKa = 11.84FEE78 pKa = 5.11AGNDD82 pKa = 3.61DD83 pKa = 3.61PDD85 pKa = 5.57GYY87 pKa = 11.37VIAGKK92 pKa = 10.38RR93 pKa = 11.84KK94 pKa = 7.16TNSARR99 pKa = 11.84GFGGNVPVAVFLDD112 pKa = 3.71DD113 pKa = 3.73GMIIRR118 pKa = 11.84GVRR121 pKa = 11.84LLEE124 pKa = 4.28NNEE127 pKa = 3.85TPRR130 pKa = 11.84FLRR133 pKa = 11.84RR134 pKa = 11.84MDD136 pKa = 3.34EE137 pKa = 3.94EE138 pKa = 4.37RR139 pKa = 11.84FLEE142 pKa = 4.41RR143 pKa = 11.84WNGRR147 pKa = 11.84HH148 pKa = 6.49LYY150 pKa = 10.3DD151 pKa = 3.6PQKK154 pKa = 10.91RR155 pKa = 11.84IDD157 pKa = 3.69ASSGASYY164 pKa = 8.44TARR167 pKa = 11.84AVISNVNNTLAALLDD182 pKa = 4.38RR183 pKa = 11.84KK184 pKa = 9.66PLPPPRR190 pKa = 11.84RR191 pKa = 11.84FAADD195 pKa = 3.54YY196 pKa = 10.23LGEE199 pKa = 4.22SAVLLVTIMSLACFVSPQSTRR220 pKa = 11.84RR221 pKa = 11.84IRR223 pKa = 11.84IPLLLLSIAVLGIWQGAFLSLALLYY248 pKa = 10.35RR249 pKa = 11.84WLLFGASPLVRR260 pKa = 11.84FGIFAAVVLSLLLPFLTNRR279 pKa = 11.84RR280 pKa = 11.84FYY282 pKa = 10.75CAYY285 pKa = 10.0LCPFGAAQEE294 pKa = 4.12LAGRR298 pKa = 11.84LGMRR302 pKa = 11.84KK303 pKa = 7.86WAIPEE308 pKa = 3.89RR309 pKa = 11.84FLRR312 pKa = 11.84PARR315 pKa = 11.84WARR318 pKa = 11.84RR319 pKa = 11.84GILAAAVVLLLILPHH334 pKa = 6.7FEE336 pKa = 4.21LADD339 pKa = 3.56IEE341 pKa = 4.64PFTAFLIRR349 pKa = 11.84SASAASLTLAGLSLAASFFFQRR371 pKa = 11.84PWCRR375 pKa = 11.84LLCPTGEE382 pKa = 4.19LLSILRR388 pKa = 11.84RR389 pKa = 11.84PVRR392 pKa = 11.84YY393 pKa = 8.43PKK395 pKa = 10.24RR396 pKa = 11.84VLRR399 pKa = 11.84RR400 pKa = 11.84NPPGCRR406 pKa = 3.24

Molecular weight: 45.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2358

0

2358

804626

29

1938

341.2

37.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.404 ± 0.059

1.131 ± 0.022

5.607 ± 0.038

6.355 ± 0.046

4.201 ± 0.032

7.915 ± 0.04

1.76 ± 0.023

5.86 ± 0.041

4.775 ± 0.056

9.112 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.019

3.72 ± 0.036

4.487 ± 0.034

3.079 ± 0.025

6.655 ± 0.05

5.536 ± 0.036

5.712 ± 0.036

7.087 ± 0.037

1.267 ± 0.023

3.805 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski