Pusillimonas sp. EA3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pusillimonas; unclassified Pusillimonas

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2949 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368WWC4|A0A368WWC4_9BURK Feruloyl-CoA synthase OS=Pusillimonas sp. EA3 OX=2183983 GN=DER48_10417 PE=4 SV=1
MM1 pKa = 7.49AVVKK5 pKa = 10.1YY6 pKa = 10.14ISAEE10 pKa = 4.11GTVTEE15 pKa = 4.91LDD17 pKa = 3.46VSPGVNLMQAAVNGGVDD34 pKa = 4.65GIIGEE39 pKa = 4.6CGGSAMCATCHH50 pKa = 6.25VYY52 pKa = 10.49VDD54 pKa = 4.03EE55 pKa = 5.31KK56 pKa = 11.34DD57 pKa = 3.42VGKK60 pKa = 10.23FDD62 pKa = 4.29PKK64 pKa = 10.46SAPEE68 pKa = 4.08EE69 pKa = 4.03EE70 pKa = 4.29MLEE73 pKa = 4.51CTTSPVQEE81 pKa = 4.21TSRR84 pKa = 11.84LSCQLFVTDD93 pKa = 5.72DD94 pKa = 3.65GDD96 pKa = 4.06EE97 pKa = 4.08ITVYY101 pKa = 10.67LPEE104 pKa = 4.38SQQQ107 pKa = 3.1

Molecular weight: 11.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368X0I7|A0A368X0I7_9BURK Cytotoxic protein CcdB OS=Pusillimonas sp. EA3 OX=2183983 GN=DER48_101216 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2949

0

2949

975259

25

2667

330.7

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.552 ± 0.047

0.912 ± 0.014

5.213 ± 0.033

5.722 ± 0.045

3.754 ± 0.033

7.881 ± 0.046

2.302 ± 0.024

5.283 ± 0.03

3.702 ± 0.038

10.532 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.02

3.416 ± 0.029

4.863 ± 0.029

4.264 ± 0.034

6.134 ± 0.038

5.779 ± 0.034

5.39 ± 0.03

7.658 ± 0.044

1.43 ± 0.02

2.586 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski