Hoyosella subflava (strain DSM 45089 / JCM 17490 / NBRC 109087 / DQS3-9A1) (Amycolicicoccus subflavus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Hoyosella; Hoyosella subflava

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6EHV3|F6EHV3_HOYSD TetR-family transcriptional regulator OS=Hoyosella subflava (strain DSM 45089 / JCM 17490 / NBRC 109087 / DQS3-9A1) OX=443218 GN=AS9A_0444 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 5.57DD3 pKa = 4.44EE4 pKa = 5.0EE5 pKa = 5.96DD6 pKa = 3.46VTAGVGVTEE15 pKa = 4.92EE16 pKa = 4.3PCPDD20 pKa = 4.05AVNADD25 pKa = 3.62NGCIYY30 pKa = 10.74LGVLSDD36 pKa = 4.45LEE38 pKa = 4.39GGPFAEE44 pKa = 5.77LGAQIQQGQLAFWRR58 pKa = 11.84SVNEE62 pKa = 4.11DD63 pKa = 3.29GGIAGYY69 pKa = 10.49DD70 pKa = 3.46VDD72 pKa = 3.15ISRR75 pKa = 11.84YY76 pKa = 6.69TRR78 pKa = 11.84NTAYY82 pKa = 10.63SVDD85 pKa = 3.55QHH87 pKa = 5.26ATEE90 pKa = 4.15YY91 pKa = 10.96QDD93 pKa = 4.28IEE95 pKa = 4.31PHH97 pKa = 5.23VLALAMSLGTPQTEE111 pKa = 4.22SVLASMDD118 pKa = 3.34SSNLVAVPGSWWSGLHH134 pKa = 6.91FDD136 pKa = 5.03DD137 pKa = 5.65ADD139 pKa = 3.91RR140 pKa = 11.84GLILEE145 pKa = 4.71SGYY148 pKa = 10.71SYY150 pKa = 10.08CTEE153 pKa = 4.13SVIGLDD159 pKa = 3.4WFSEE163 pKa = 4.8EE164 pKa = 4.28YY165 pKa = 8.94GTPEE169 pKa = 3.6TLLTVGYY176 pKa = 8.97PGDD179 pKa = 3.97YY180 pKa = 10.9GSDD183 pKa = 3.41SAVGATRR190 pKa = 11.84WAEE193 pKa = 3.98ANDD196 pKa = 3.67VEE198 pKa = 5.09VLAQIDD204 pKa = 3.89TAPNAMVQNQDD215 pKa = 3.09AAVEE219 pKa = 4.41QILSQQPDD227 pKa = 3.53VVLIATAPGEE237 pKa = 3.95AAEE240 pKa = 4.4IVAKK244 pKa = 10.37AVSRR248 pKa = 11.84GYY250 pKa = 10.61DD251 pKa = 3.33GRR253 pKa = 11.84FLGSVPTWNPRR264 pKa = 11.84LLEE267 pKa = 4.01TAAIGALTARR277 pKa = 11.84YY278 pKa = 8.84NHH280 pKa = 6.11VGPWQGWDD288 pKa = 3.31GDD290 pKa = 4.33SAALSAIRR298 pKa = 11.84EE299 pKa = 4.36SLDD302 pKa = 3.13GRR304 pKa = 11.84LPDD307 pKa = 3.38NQGYY311 pKa = 9.45IFGWVWSYY319 pKa = 10.79PLKK322 pKa = 10.92SLLEE326 pKa = 4.03AAADD330 pKa = 3.94DD331 pKa = 4.91GEE333 pKa = 4.44LSRR336 pKa = 11.84SALRR340 pKa = 11.84AAIDD344 pKa = 3.75GLEE347 pKa = 3.82VDD349 pKa = 4.83YY350 pKa = 11.52EE351 pKa = 4.34GALPNRR357 pKa = 11.84TFGEE361 pKa = 4.33PPAFDD366 pKa = 3.95EE367 pKa = 4.4MSATISVPDD376 pKa = 3.84AEE378 pKa = 4.73VEE380 pKa = 4.06LGTRR384 pKa = 11.84AIADD388 pKa = 3.59GVTATTEE395 pKa = 3.95VEE397 pKa = 3.85YY398 pKa = 10.51DD399 pKa = 3.81APCVQPP405 pKa = 4.64

Molecular weight: 43.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6EPG9|F6EPG9_HOYSD Binding-protein-dependent transport systems inner membrane component OS=Hoyosella subflava (strain DSM 45089 / JCM 17490 / NBRC 109087 / DQS3-9A1) OX=443218 GN=AS9A_0950 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84SIISARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.56GRR42 pKa = 11.84AEE44 pKa = 3.81LAVV47 pKa = 3.46

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4703

0

4703

1471282

37

7481

312.8

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.481 ± 0.048

0.78 ± 0.012

5.933 ± 0.03

5.903 ± 0.034

3.188 ± 0.022

8.693 ± 0.046

2.265 ± 0.019

4.671 ± 0.027

2.283 ± 0.029

9.97 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.055 ± 0.016

2.23 ± 0.018

5.461 ± 0.029

3.002 ± 0.02

7.275 ± 0.043

5.877 ± 0.023

5.938 ± 0.022

8.479 ± 0.033

1.435 ± 0.016

2.08 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski