Burkholderia phage Bcep781 (isolate -/United States/Beer/1978) (Bacteriophage Bcep781)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Naesvirus; Burkholderia virus Bcep781

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8HAL2|Q8HAL2_BPB78 Gp50 OS=Burkholderia phage Bcep781 (isolate -/United States/Beer/1978) OX=1283334 GN=Bcep781-50 PE=4 SV=1
MM1 pKa = 8.15DD2 pKa = 5.38SLALDD7 pKa = 3.94PATWDD12 pKa = 3.26ITTDD16 pKa = 3.05AYY18 pKa = 11.11GNLATVGDD26 pKa = 4.62ATPGDD31 pKa = 4.33YY32 pKa = 10.97SGGAYY37 pKa = 10.1RR38 pKa = 11.84MAQDD42 pKa = 3.65VACRR46 pKa = 11.84CMSWLGEE53 pKa = 4.15VYY55 pKa = 10.91YY56 pKa = 10.77DD57 pKa = 3.21ATQGIRR63 pKa = 11.84YY64 pKa = 8.89DD65 pKa = 3.73QVLGLAPNLVLVQALYY81 pKa = 11.16VNEE84 pKa = 4.06ALKK87 pKa = 10.52VAPVAQAVANLAYY100 pKa = 10.56VAGAQRR106 pKa = 11.84RR107 pKa = 11.84VTGQLVVSDD116 pKa = 4.38GSVTTGVVQLL126 pKa = 4.28

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q546W1|Q546W1_BPB78 Gp57 OS=Burkholderia phage Bcep781 (isolate -/United States/Beer/1978) OX=1283334 GN=Bcep781-57 PE=4 SV=1
MM1 pKa = 7.19TAPDD5 pKa = 4.78RR6 pKa = 11.84INGVSRR12 pKa = 11.84ARR14 pKa = 11.84LEE16 pKa = 4.14RR17 pKa = 11.84EE18 pKa = 3.93CASKK22 pKa = 10.69NRR24 pKa = 11.84YY25 pKa = 8.46PDD27 pKa = 3.36EE28 pKa = 4.15LTARR32 pKa = 11.84ASGLHH37 pKa = 5.51HH38 pKa = 6.74QDD40 pKa = 3.02RR41 pKa = 11.84NKK43 pKa = 10.8LDD45 pKa = 3.75GLWVYY50 pKa = 10.7ACKK53 pKa = 10.42HH54 pKa = 5.16CAGWHH59 pKa = 4.17LTRR62 pKa = 11.84RR63 pKa = 11.84NNGPRR68 pKa = 11.84WRR70 pKa = 11.84VV71 pKa = 2.94

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

15012

47

873

227.5

24.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.035 ± 0.431

0.999 ± 0.111

6.002 ± 0.283

4.41 ± 0.355

3.584 ± 0.172

8.153 ± 0.357

1.739 ± 0.16

4.723 ± 0.176

3.131 ± 0.21

8.213 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.118

3.804 ± 0.305

5.556 ± 0.25

4.123 ± 0.192

6.168 ± 0.441

5.149 ± 0.264

6.788 ± 0.454

6.701 ± 0.216

1.545 ± 0.093

2.891 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski