Gloeomargarita lithophora Alchichica-D10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Gloeoemargaritales; Gloeomargaritaceae; Gloeomargarita; Gloeomargarita lithophora

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3022 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0A9M4|A0A1J0A9M4_9CYAN Uncharacterized protein OS=Gloeomargarita lithophora Alchichica-D10 OX=1188229 GN=GlitD10_0316 PE=4 SV=1
MM1 pKa = 7.61NIQFRR6 pKa = 11.84EE7 pKa = 3.74VDD9 pKa = 4.03TFNLWIWVEE18 pKa = 4.53FPQPPTQEE26 pKa = 3.8EE27 pKa = 3.89QQYY30 pKa = 10.56LEE32 pKa = 5.08EE33 pKa = 5.13IFSSWFLLGKK43 pKa = 10.5LGGFNAEE50 pKa = 4.23NLPVQDD56 pKa = 4.12QGYY59 pKa = 9.8DD60 pKa = 3.6LNFFPYY66 pKa = 9.32DD67 pKa = 3.61TEE69 pKa = 4.3TSDD72 pKa = 4.43DD73 pKa = 3.45AFLAVMHH80 pKa = 6.23NMGEE84 pKa = 4.47VEE86 pKa = 4.54YY87 pKa = 9.93QDD89 pKa = 2.89HH90 pKa = 7.02WARR93 pKa = 11.84CWFDD97 pKa = 4.47LGTSDD102 pKa = 6.01GLALDD107 pKa = 3.34ILLNVLRR114 pKa = 11.84QFDD117 pKa = 3.8QEE119 pKa = 4.37YY120 pKa = 10.23VPLKK124 pKa = 9.13TVIIGGVNPDD134 pKa = 2.98WPVDD138 pKa = 3.5HH139 pKa = 7.24FEE141 pKa = 4.7PEE143 pKa = 4.08SPLADD148 pKa = 3.69DD149 pKa = 5.0FF150 pKa = 5.14

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0ABX0|A0A1J0ABX0_9CYAN 50S ribosomal protein L21 OS=Gloeomargarita lithophora Alchichica-D10 OX=1188229 GN=rplU PE=3 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.03RR4 pKa = 11.84TLEE7 pKa = 3.93GTSRR11 pKa = 11.84KK12 pKa = 9.28RR13 pKa = 11.84LRR15 pKa = 11.84RR16 pKa = 11.84SGFRR20 pKa = 11.84ARR22 pKa = 11.84MATPGGRR29 pKa = 11.84RR30 pKa = 11.84VLQARR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.1GRR40 pKa = 11.84KK41 pKa = 8.96RR42 pKa = 11.84LALVV46 pKa = 3.4

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2990

32

3022

879357

8

2424

291.0

32.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.856 ± 0.051

1.026 ± 0.016

4.484 ± 0.034

5.615 ± 0.041

3.51 ± 0.035

7.804 ± 0.057

2.104 ± 0.026

5.408 ± 0.045

3.176 ± 0.046

11.779 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.985 ± 0.02

3.337 ± 0.043

5.588 ± 0.045

6.307 ± 0.055

6.02 ± 0.038

4.929 ± 0.032

5.838 ± 0.045

7.47 ± 0.04

1.848 ± 0.027

2.916 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski