Helicobacter phage Pt1918U

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RFS0|A0A1S5RFS0_9CAUD Uncharacterized protein OS=Helicobacter phage Pt1918U OX=1852671 PE=4 SV=1
MM1 pKa = 6.92KK2 pKa = 10.4QRR4 pKa = 11.84VKK6 pKa = 10.05TVSYY10 pKa = 9.77LAKK13 pKa = 10.59AEE15 pKa = 3.91FDD17 pKa = 3.9IKK19 pKa = 11.0DD20 pKa = 3.38GSYY23 pKa = 11.15DD24 pKa = 4.01LSALPSGAEE33 pKa = 3.94VVKK36 pKa = 10.83CDD38 pKa = 3.9LEE40 pKa = 4.61VVGEE44 pKa = 4.26VVTAKK49 pKa = 10.73ASVGFKK55 pKa = 10.9DD56 pKa = 3.38EE57 pKa = 4.74AEE59 pKa = 4.36VNFFLDD65 pKa = 6.03DD66 pKa = 3.53IDD68 pKa = 5.83LGTNRR73 pKa = 11.84FNTSAKK79 pKa = 10.21CYY81 pKa = 7.59TALDD85 pKa = 3.93TKK87 pKa = 10.85VISAKK92 pKa = 10.22IINASGNAKK101 pKa = 9.76GVLRR105 pKa = 11.84VLYY108 pKa = 7.75FLPSEE113 pKa = 4.55TEE115 pKa = 3.62VEE117 pKa = 4.49YY118 pKa = 11.22

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RFS1|A0A1S5RFS1_9CAUD Uncharacterized protein OS=Helicobacter phage Pt1918U OX=1852671 PE=4 SV=1
MM1 pKa = 6.68NTNRR5 pKa = 11.84TPNITVIAFGRR16 pKa = 11.84RR17 pKa = 11.84KK18 pKa = 9.4EE19 pKa = 4.09RR20 pKa = 11.84EE21 pKa = 3.78KK22 pKa = 11.37SKK24 pKa = 9.72MSHH27 pKa = 5.67YY28 pKa = 10.2RR29 pKa = 11.84HH30 pKa = 5.75KK31 pKa = 10.93QKK33 pKa = 10.77NFKK36 pKa = 10.18PKK38 pKa = 10.57VKK40 pKa = 9.58TEE42 pKa = 4.31CILFQRR48 pKa = 11.84VFLKK52 pKa = 10.41ILEE55 pKa = 4.11ILKK58 pKa = 9.39EE59 pKa = 3.98QEE61 pKa = 3.95GKK63 pKa = 9.9SRR65 pKa = 11.84SVIIEE70 pKa = 3.58RR71 pKa = 11.84MIIYY75 pKa = 10.08FLEE78 pKa = 4.36KK79 pKa = 10.53EE80 pKa = 4.38KK81 pKa = 11.29GNKK84 pKa = 9.81DD85 pKa = 2.83EE86 pKa = 4.33TAWNKK91 pKa = 9.94SKK93 pKa = 10.75RR94 pKa = 11.84SYY96 pKa = 11.0RR97 pKa = 11.84RR98 pKa = 11.84TLNNYY103 pKa = 9.25KK104 pKa = 10.25KK105 pKa = 9.91EE106 pKa = 4.18ANVKK110 pKa = 10.09RR111 pKa = 11.84KK112 pKa = 8.7QLQKK116 pKa = 10.97AKK118 pKa = 10.37EE119 pKa = 4.05DD120 pKa = 3.73EE121 pKa = 4.42KK122 pKa = 11.47KK123 pKa = 10.26KK124 pKa = 10.89ALYY127 pKa = 9.28ICKK130 pKa = 9.9SSPFSYY136 pKa = 10.17FRR138 pKa = 11.84RR139 pKa = 11.84YY140 pKa = 9.18

Molecular weight: 17.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

9172

45

1576

269.8

31.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.389 ± 0.355

0.807 ± 0.224

5.026 ± 0.319

8.657 ± 0.305

5.135 ± 0.275

4.099 ± 0.253

1.188 ± 0.155

7.076 ± 0.308

11.001 ± 0.425

10.499 ± 0.345

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.853 ± 0.19

8.352 ± 0.383

2.191 ± 0.182

4.23 ± 0.293

4.176 ± 0.209

6.487 ± 0.268

4.437 ± 0.272

4.328 ± 0.379

0.458 ± 0.087

3.609 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski