Haloarcula hispanica pleomorphic virus 4

Taxonomy: Viruses; Monodnaviria; Trapavirae; Saleviricota; Huolimaviricetes; Haloruvirales; Pleolipoviridae; Betapleolipovirus; Betapleolipovirus HHPV4

Average proteome isoelectric point is 5.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0QEG3|A0A2P0QEG3_9VIRU Uncharacterized protein OS=Haloarcula hispanica pleomorphic virus 4 OX=1980140 PE=4 SV=1
MM1 pKa = 7.5AALAASPTMFGKK13 pKa = 9.89IDD15 pKa = 3.83IEE17 pKa = 4.21DD18 pKa = 3.8ALAGVIFTASAFVTNGIASISLLGYY43 pKa = 10.45DD44 pKa = 4.29LAASVFTVQGTSIDD58 pKa = 3.82LAFLLSLVALAMAYY72 pKa = 7.68ATNRR76 pKa = 11.84VNEE79 pKa = 4.26SRR81 pKa = 11.84NKK83 pKa = 10.3NYY85 pKa = 9.95QLDD88 pKa = 3.87TDD90 pKa = 4.62LVDD93 pKa = 3.32IAKK96 pKa = 10.58GSATVEE102 pKa = 4.21TYY104 pKa = 10.68LALGTLVIVLFTGLNILGAQDD125 pKa = 3.35IVVGSAAIGLTVVAVEE141 pKa = 3.67AAGYY145 pKa = 9.17YY146 pKa = 9.57VISYY150 pKa = 10.2LGG152 pKa = 3.24

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0QEK3|A0A2P0QEK3_9VIRU Uncharacterized protein OS=Haloarcula hispanica pleomorphic virus 4 OX=1980140 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.06IVTHH6 pKa = 6.63AFSPPRR12 pKa = 11.84TDD14 pKa = 3.32RR15 pKa = 11.84PSSKK19 pKa = 10.14LRR21 pKa = 11.84AAQPEE26 pKa = 4.35QIGLRR31 pKa = 11.84VRR33 pKa = 11.84ASIPSASARR42 pKa = 11.84QVAQLPSSEE51 pKa = 5.76LFDD54 pKa = 4.79CDD56 pKa = 3.0ASVFVRR62 pKa = 11.84GCSPVMVDD70 pKa = 3.83SLMAYY75 pKa = 9.64RR76 pKa = 11.84ARR78 pKa = 11.84SHH80 pKa = 6.82LFGRR84 pKa = 11.84CTRR87 pKa = 11.84PPVRR91 pKa = 11.84HH92 pKa = 5.7EE93 pKa = 3.64PRR95 pKa = 11.84RR96 pKa = 11.84VIFDD100 pKa = 4.51RR101 pKa = 11.84IGCFAYY107 pKa = 10.77VPTTYY112 pKa = 10.66CCINATLFQQ121 pKa = 4.52

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

4545

38

613

189.4

21.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.679 ± 0.658

1.188 ± 0.259

7.217 ± 0.377

8.031 ± 0.557

2.728 ± 0.299

7.173 ± 0.414

2.178 ± 0.281

5.281 ± 0.425

3.916 ± 0.295

7.129 ± 0.386

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.958 ± 0.204

4.07 ± 0.529

4.752 ± 0.404

4.664 ± 0.352

6.777 ± 0.777

6.491 ± 0.561

6.579 ± 0.486

6.579 ± 0.382

1.496 ± 0.209

4.114 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski