Proteome-pI 2.0 - Proteome Isoelectric Point Database

Microbacterium kyungheense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3787 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A543F2C1|A0A543F2C1_9MICO DNA-binding PadR family transcriptional regulator OS=Microbacterium kyungheense OX=1263636 GN=FB391_2035 PE=4 SV=1

MM1 pKa = 7.5PANRR5 pKa = 11.84LLGTVAVLALSALALTACAGSPEE28 pKa = 4.55ANPDD32 pKa = 3.43PTTSSVPTTAAPEE45 pKa = 4.21PSASAAPEE53 pKa = 4.04PTADD57 pKa = 4.17AAPTCEE63 pKa = 3.97TLISDD68 pKa = 4.42SLVADD73 pKa = 3.74YY74 pKa = 11.27EE75 pKa = 4.65KK76 pKa = 10.66IGVTAQEE83 pKa = 4.36SPFYY87 pKa = 10.46IAGQAIEE94 pKa = 4.49GGLRR98 pKa = 11.84CMWANFDD105 pKa = 4.24QPAGDD110 pKa = 3.63SGQIYY115 pKa = 9.9GWAKK119 pKa = 10.3MSDD122 pKa = 3.49ADD124 pKa = 5.34ADD126 pKa = 3.94AAQQALLAEE135 pKa = 4.53GWVRR139 pKa = 11.84EE140 pKa = 4.07DD141 pKa = 3.44AADD144 pKa = 3.62GGVYY148 pKa = 8.58ITEE151 pKa = 4.16SHH153 pKa = 6.7DD154 pKa = 3.5TAIVTDD160 pKa = 3.87DD161 pKa = 3.47EE162 pKa = 5.56GYY164 pKa = 11.44GMTYY168 pKa = 10.59LFAAGQVTVADD179 pKa = 4.34TKK181 pKa = 11.0QGLLLIEE188 pKa = 4.16WPKK191 pKa = 11.24AA192 pKa = 3.39

Molecular weight: 19.77 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A543EAU7|A0A543EAU7_9MICO Two-component system sensor histidine kinase DesK OS=Microbacterium kyungheense OX=1263636 GN=FB391_3852 PE=4 SV=1

MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.56GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.27 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3787	0	3787	1287887	26	10898	340.1	36.28

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
14.159 ± 0.09	0.467 ± 0.01	6.595 ± 0.04	5.423 ± 0.045	3.126 ± 0.026	9.062 ± 0.054	1.951 ± 0.018	4.278 ± 0.027	1.734 ± 0.028	9.883 ± 0.057

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.705 ± 0.019	1.811 ± 0.023	5.589 ± 0.035	2.68 ± 0.022	7.31 ± 0.064	5.318 ± 0.04	6.09 ± 0.062	9.155 ± 0.038	1.636 ± 0.02	2.028 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski