Isoptericola variabilis (strain 225)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Promicromonosporaceae; Isoptericola; Isoptericola variabilis

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2879 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6FPW0|F6FPW0_ISOV2 Monosaccharide-transporting ATPase OS=Isoptericola variabilis (strain 225) OX=743718 GN=Isova_0965 PE=4 SV=1
MM1 pKa = 7.0TPRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84LAGIGAFALASALVLSACGGGGGDD30 pKa = 3.67NTDD33 pKa = 3.28TGEE36 pKa = 4.45GGDD39 pKa = 3.67AGATEE44 pKa = 5.17GGATTGIVTVNGTEE58 pKa = 4.04PQNPLTPGMTNEE70 pKa = 4.19VGGGLAVKK78 pKa = 10.49NIFAGLVYY86 pKa = 10.5YY87 pKa = 10.43DD88 pKa = 4.37AEE90 pKa = 4.65GATHH94 pKa = 6.69YY95 pKa = 11.13EE96 pKa = 3.93MAEE99 pKa = 4.21SIEE102 pKa = 4.98SEE104 pKa = 4.9DD105 pKa = 3.67NQNWTITIQDD115 pKa = 3.05GWTFSDD121 pKa = 3.93GTPVTAQSYY130 pKa = 8.82VDD132 pKa = 3.19AWKK135 pKa = 8.91YY136 pKa = 8.08TALLSNGQNQQYY148 pKa = 9.07FFHH151 pKa = 7.6DD152 pKa = 3.9FAGWSEE158 pKa = 4.4TEE160 pKa = 3.98DD161 pKa = 3.51SEE163 pKa = 4.94IAVEE167 pKa = 4.42VVDD170 pKa = 4.46DD171 pKa = 3.71HH172 pKa = 6.4TFTVEE177 pKa = 3.79LAFPNAEE184 pKa = 4.1FEE186 pKa = 4.3RR187 pKa = 11.84TLGYY191 pKa = 8.34TAFMPMPEE199 pKa = 4.46VFFEE203 pKa = 4.4TPDD206 pKa = 3.27IYY208 pKa = 11.41SDD210 pKa = 3.76APVGNGPYY218 pKa = 9.95VVEE221 pKa = 4.27SWEE224 pKa = 4.15HH225 pKa = 5.68EE226 pKa = 4.15NVISLRR232 pKa = 11.84PNPDD236 pKa = 2.82YY237 pKa = 10.84TGPRR241 pKa = 11.84TPQNGGIDD249 pKa = 3.22IVIYY253 pKa = 10.17TDD255 pKa = 3.53EE256 pKa = 4.02NTAYY260 pKa = 11.08NDD262 pKa = 4.03LLSDD266 pKa = 3.78NLDD269 pKa = 3.77IITNVPASAFQTFEE283 pKa = 3.9SDD285 pKa = 3.3LGDD288 pKa = 3.35RR289 pKa = 11.84AVNQPAAVIQVIQVPAWLPEE309 pKa = 4.01FEE311 pKa = 5.09GEE313 pKa = 3.96AGNLRR318 pKa = 11.84RR319 pKa = 11.84QAISKK324 pKa = 10.61AIDD327 pKa = 3.2RR328 pKa = 11.84EE329 pKa = 4.48QVTEE333 pKa = 4.15TVFAGTRR340 pKa = 11.84TPATDD345 pKa = 3.5FTSPVIDD352 pKa = 5.19GYY354 pKa = 11.43SEE356 pKa = 5.76DD357 pKa = 3.72IPGSEE362 pKa = 4.11VLQYY366 pKa = 11.01DD367 pKa = 3.74PEE369 pKa = 4.57GAKK372 pKa = 9.7QLWDD376 pKa = 3.58EE377 pKa = 4.61AEE379 pKa = 4.31GMDD382 pKa = 4.84PLGDD386 pKa = 3.64YY387 pKa = 8.89TLQLASNADD396 pKa = 3.64SDD398 pKa = 3.84HH399 pKa = 5.24QTWVEE404 pKa = 3.87AVCNNIRR411 pKa = 11.84TVLEE415 pKa = 4.32IEE417 pKa = 4.57CEE419 pKa = 4.25FYY421 pKa = 10.41PYY423 pKa = 9.02PTFAEE428 pKa = 4.22FLDD431 pKa = 3.87ARR433 pKa = 11.84DD434 pKa = 3.69AGEE437 pKa = 4.02VPGLFRR443 pKa = 11.84GGWQADD449 pKa = 3.56WPAMSNFLGPVYY461 pKa = 10.0GTGAGSNDD469 pKa = 3.01MQYY472 pKa = 11.53SNEE475 pKa = 3.98EE476 pKa = 3.49FDD478 pKa = 5.13AKK480 pKa = 10.02LTEE483 pKa = 4.45AGAAEE488 pKa = 4.33SPEE491 pKa = 4.26EE492 pKa = 3.73ATEE495 pKa = 4.37LYY497 pKa = 10.66KK498 pKa = 10.52EE499 pKa = 4.04AQEE502 pKa = 4.07ILFRR506 pKa = 11.84DD507 pKa = 3.95LPGIPLWYY515 pKa = 10.49NNATAGYY522 pKa = 9.13AATVDD527 pKa = 3.48NVQFGWDD534 pKa = 3.64SDD536 pKa = 4.75PILHH540 pKa = 6.56EE541 pKa = 4.3VTKK544 pKa = 11.05SEE546 pKa = 4.04

Molecular weight: 59.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6FV08|F6FV08_ISOV2 Fmu (Sun) domain protein OS=Isoptericola variabilis (strain 225) OX=743718 GN=Isova_1595 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2879

0

2879

987482

30

3247

343.0

36.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.278 ± 0.077

0.541 ± 0.01

6.508 ± 0.045

5.985 ± 0.045

2.593 ± 0.028

9.355 ± 0.049

2.151 ± 0.023

2.798 ± 0.027

1.513 ± 0.026

10.059 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.591 ± 0.015

1.522 ± 0.022

6.057 ± 0.035

2.495 ± 0.023

7.983 ± 0.049

4.702 ± 0.029

6.19 ± 0.039

10.253 ± 0.053

1.551 ± 0.018

1.873 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski