Xanthomonas phage Xf409

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Xylivirus; Xanthomonas virus Xf109

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6DXV6|A0A1W6DXV6_9VIRU Uncharacterized protein OS=Xanthomonas phage Xf409 OX=1979540 PE=4 SV=1
MM1 pKa = 7.94GIGQGSGDD9 pKa = 3.41GRR11 pKa = 11.84VDD13 pKa = 3.68LVGRR17 pKa = 11.84LAGGLRR23 pKa = 11.84DD24 pKa = 3.7CLRR27 pKa = 11.84GFRR30 pKa = 11.84IMGWLARR37 pKa = 11.84VFASAIARR45 pKa = 11.84RR46 pKa = 11.84LAYY49 pKa = 10.38VLVAATLAWCGIGCASAEE67 pKa = 4.1DD68 pKa = 4.89FGTQGAAYY76 pKa = 8.25SACMSQTAAYY86 pKa = 8.6LASRR90 pKa = 11.84GRR92 pKa = 11.84GANDD96 pKa = 3.61RR97 pKa = 11.84NPEE100 pKa = 4.17CNVEE104 pKa = 4.26QGSHH108 pKa = 6.48AYY110 pKa = 9.94RR111 pKa = 11.84GQFEE115 pKa = 4.32TRR117 pKa = 11.84DD118 pKa = 3.73CSSCDD123 pKa = 2.74WYY125 pKa = 10.62RR126 pKa = 11.84AYY128 pKa = 10.79YY129 pKa = 10.43GYY131 pKa = 10.27FAWSKK136 pKa = 11.32GCDD139 pKa = 3.39QEE141 pKa = 4.35PDD143 pKa = 3.69YY144 pKa = 11.04TGSGPWGTYY153 pKa = 9.79VGTARR158 pKa = 11.84NGSIGCRR165 pKa = 11.84NGCDD169 pKa = 3.53GVWFGNSDD177 pKa = 4.83DD178 pKa = 3.88TMTWNATGAICPKK191 pKa = 10.5DD192 pKa = 3.59PEE194 pKa = 4.47KK195 pKa = 10.66TCDD198 pKa = 3.33AMTGGYY204 pKa = 8.06VWNGYY209 pKa = 10.15LGVCEE214 pKa = 4.54PPPNEE219 pKa = 4.34KK220 pKa = 10.53CPEE223 pKa = 4.08GQVPDD228 pKa = 4.2GKK230 pKa = 10.9GGCSPNKK237 pKa = 9.93CPEE240 pKa = 3.85GMLLQADD247 pKa = 4.98GTCAPKK253 pKa = 10.83KK254 pKa = 9.58NDD256 pKa = 3.67CPAGQIRR263 pKa = 11.84SPSGSCLPGDD273 pKa = 4.19GQCAAGEE280 pKa = 4.2VRR282 pKa = 11.84GPDD285 pKa = 3.62GTCKK289 pKa = 10.24KK290 pKa = 10.69DD291 pKa = 3.18GDD293 pKa = 4.3GDD295 pKa = 4.23GEE297 pKa = 4.16PDD299 pKa = 3.55EE300 pKa = 5.35PGEE303 pKa = 4.14GDD305 pKa = 2.97KK306 pKa = 11.39SQFSGGDD313 pKa = 3.37SCDD316 pKa = 3.89SPPSCSGDD324 pKa = 3.87AIMCGQARR332 pKa = 11.84IQWRR336 pKa = 11.84IDD338 pKa = 3.14CNTRR342 pKa = 11.84RR343 pKa = 11.84DD344 pKa = 3.65VNITGGSCAAMPVCVGTNCKK364 pKa = 9.99ALEE367 pKa = 3.92YY368 pKa = 10.61SQLLMQWRR376 pKa = 11.84AACALEE382 pKa = 4.09KK383 pKa = 10.52AANNSGGGTGNNADD397 pKa = 3.25VKK399 pKa = 10.58AIRR402 pKa = 11.84DD403 pKa = 4.11AITGNGTADD412 pKa = 2.97IGADD416 pKa = 3.86GKK418 pKa = 10.07PADD421 pKa = 4.27AFSDD425 pKa = 3.52EE426 pKa = 4.31SGYY429 pKa = 10.46GQEE432 pKa = 4.84GYY434 pKa = 7.72PTGEE438 pKa = 5.06LDD440 pKa = 3.66TQGFGYY446 pKa = 10.49SRR448 pKa = 11.84TCPTIPDD455 pKa = 3.49VAVFGQTLHH464 pKa = 6.82FDD466 pKa = 3.53TSKK469 pKa = 9.79FCQWMVLGGQIVLVMASLVSLRR491 pKa = 11.84LMSQGGSAA499 pKa = 3.86

Molecular weight: 52.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6DY99|A0A1W6DY99_9VIRU Uncharacterized protein OS=Xanthomonas phage Xf409 OX=1979540 PE=4 SV=1
MM1 pKa = 7.21SAEE4 pKa = 4.22NEE6 pKa = 4.36LIDD9 pKa = 4.9LVRR12 pKa = 11.84AGGKK16 pKa = 9.71FSSDD20 pKa = 2.66NALAQKK26 pKa = 10.69LGITRR31 pKa = 11.84AMVSSWRR38 pKa = 11.84SGRR41 pKa = 11.84YY42 pKa = 9.32AMPDD46 pKa = 3.28DD47 pKa = 5.94QIAQLCALAKK57 pKa = 10.39LDD59 pKa = 3.74GASWMARR66 pKa = 11.84IHH68 pKa = 5.15TEE70 pKa = 3.65RR71 pKa = 11.84AGSATEE77 pKa = 3.71RR78 pKa = 11.84ALWQSILDD86 pKa = 3.67RR87 pKa = 11.84LTPITAVVGALALCEE102 pKa = 3.95IRR104 pKa = 11.84NRR106 pKa = 11.84AVDD109 pKa = 3.32AALHH113 pKa = 5.65RR114 pKa = 11.84RR115 pKa = 11.84GLLLQPTPQEE125 pKa = 4.41ADD127 pKa = 2.55WTMTFDD133 pKa = 2.91TYY135 pKa = 11.54EE136 pKa = 4.25RR137 pKa = 11.84VDD139 pKa = 4.05LTGPWAGFGFQGHH152 pKa = 6.07RR153 pKa = 11.84FFTPEE158 pKa = 4.2NYY160 pKa = 10.47DD161 pKa = 3.09IDD163 pKa = 4.44PCGMRR168 pKa = 11.84YY169 pKa = 8.0WALTCAIARR178 pKa = 11.84EE179 pKa = 4.08WSLMMSEE186 pKa = 4.18EE187 pKa = 4.07RR188 pKa = 11.84NARR191 pKa = 11.84SANPRR196 pKa = 11.84TPTATRR202 pKa = 11.84SPGSRR207 pKa = 11.84ISADD211 pKa = 3.04ANVIYY216 pKa = 10.62LRR218 pKa = 11.84DD219 pKa = 3.39VLRR222 pKa = 11.84RR223 pKa = 11.84RR224 pKa = 11.84RR225 pKa = 11.84EE226 pKa = 3.68TRR228 pKa = 11.84LSVGRR233 pKa = 11.84DD234 pKa = 3.15PGLADD239 pKa = 3.28SGKK242 pKa = 10.03RR243 pKa = 11.84SPAGRR248 pKa = 11.84GRR250 pKa = 11.84RR251 pKa = 11.84RR252 pKa = 11.84PRR254 pKa = 11.84RR255 pKa = 11.84GG256 pKa = 2.87

Molecular weight: 28.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2591

61

499

185.1

20.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.35 ± 0.927

2.239 ± 0.828

5.288 ± 0.599

3.782 ± 0.395

3.396 ± 0.54

9.726 ± 1.281

1.621 ± 0.349

3.705 ± 0.359

3.782 ± 0.601

8.452 ± 1.17

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.236

2.277 ± 0.378

5.21 ± 0.438

4.052 ± 0.512

8.066 ± 1.096

6.059 ± 0.511

5.403 ± 0.721

6.561 ± 0.994

2.74 ± 0.424

2.74 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski