Amino acid dipepetide frequency for Novosphingobium sp. 9U

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.254AlaAla: 18.254 ± 0.19
1.254AlaCys: 1.254 ± 0.034
7.103AlaAsp: 7.103 ± 0.075
7.507AlaGlu: 7.507 ± 0.081
4.221AlaPhe: 4.221 ± 0.063
11.164AlaGly: 11.164 ± 0.111
2.45AlaHis: 2.45 ± 0.051
6.347AlaIle: 6.347 ± 0.074
4.101AlaLys: 4.101 ± 0.067
13.783AlaLeu: 13.783 ± 0.142
3.773AlaMet: 3.773 ± 0.059
3.041AlaAsn: 3.041 ± 0.049
6.403AlaPro: 6.403 ± 0.094
4.967AlaGln: 4.967 ± 0.078
9.67AlaArg: 9.67 ± 0.098
7.097AlaSer: 7.097 ± 0.076
6.42AlaThr: 6.42 ± 0.075
8.643AlaVal: 8.643 ± 0.078
1.728AlaTrp: 1.728 ± 0.04
2.599AlaTyr: 2.599 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.13CysAla: 1.13 ± 0.035
0.132CysCys: 0.132 ± 0.01
0.595CysAsp: 0.595 ± 0.022
0.536CysGlu: 0.536 ± 0.02
0.319CysPhe: 0.319 ± 0.014
0.957CysGly: 0.957 ± 0.028
0.251CysHis: 0.251 ± 0.013
0.357CysIle: 0.357 ± 0.016
0.228CysLys: 0.228 ± 0.014
0.82CysLeu: 0.82 ± 0.026
0.207CysMet: 0.207 ± 0.014
0.239CysAsn: 0.239 ± 0.015
0.516CysPro: 0.516 ± 0.021
0.262CysGln: 0.262 ± 0.012
0.681CysArg: 0.681 ± 0.025
0.567CysSer: 0.567 ± 0.022
0.446CysThr: 0.446 ± 0.019
0.645CysVal: 0.645 ± 0.019
0.119CysTrp: 0.119 ± 0.009
0.195CysTyr: 0.195 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.393AspAla: 7.393 ± 0.075
0.544AspCys: 0.544 ± 0.018
3.096AspAsp: 3.096 ± 0.054
3.401AspGlu: 3.401 ± 0.061
2.078AspPhe: 2.078 ± 0.045
5.081AspGly: 5.081 ± 0.066
1.291AspHis: 1.291 ± 0.036
2.5AspIle: 2.5 ± 0.045
1.84AspLys: 1.84 ± 0.04
5.643AspLeu: 5.643 ± 0.066
1.31AspMet: 1.31 ± 0.031
1.342AspAsn: 1.342 ± 0.03
3.648AspPro: 3.648 ± 0.055
1.778AspGln: 1.778 ± 0.034
4.489AspArg: 4.489 ± 0.062
2.15AspSer: 2.15 ± 0.041
2.713AspThr: 2.713 ± 0.044
4.167AspVal: 4.167 ± 0.062
1.064AspTrp: 1.064 ± 0.03
1.489AspTyr: 1.489 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
7.796GluAla: 7.796 ± 0.088
0.393GluCys: 0.393 ± 0.016
2.802GluAsp: 2.802 ± 0.052
2.962GluGlu: 2.962 ± 0.053
1.609GluPhe: 1.609 ± 0.037
4.558GluGly: 4.558 ± 0.061
1.389GluHis: 1.389 ± 0.033
2.801GluIle: 2.801 ± 0.046
1.763GluLys: 1.763 ± 0.04
5.348GluLeu: 5.348 ± 0.064
1.317GluMet: 1.317 ± 0.032
1.309GluAsn: 1.309 ± 0.027
2.879GluPro: 2.879 ± 0.047
2.283GluGln: 2.283 ± 0.044
5.156GluArg: 5.156 ± 0.075
2.268GluSer: 2.268 ± 0.037
2.919GluThr: 2.919 ± 0.043
3.831GluVal: 3.831 ± 0.049
0.861GluTrp: 0.861 ± 0.026
1.067GluTyr: 1.067 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.784PheAla: 4.784 ± 0.057
0.37PheCys: 0.37 ± 0.016
2.53PheAsp: 2.53 ± 0.04
2.009PheGlu: 2.009 ± 0.039
1.202PhePhe: 1.202 ± 0.035
3.685PheGly: 3.685 ± 0.061
0.735PheHis: 0.735 ± 0.024
1.3PheIle: 1.3 ± 0.034
0.862PheLys: 0.862 ± 0.025
2.951PheLeu: 2.951 ± 0.048
0.671PheMet: 0.671 ± 0.024
0.994PheAsn: 0.994 ± 0.028
1.437PhePro: 1.437 ± 0.032
0.943PheGln: 0.943 ± 0.027
2.122PheArg: 2.122 ± 0.04
2.006PheSer: 2.006 ± 0.044
1.914PheThr: 1.914 ± 0.04
2.666PheVal: 2.666 ± 0.045
0.521PheTrp: 0.521 ± 0.021
0.926PheTyr: 0.926 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
9.879GlyAla: 9.879 ± 0.093
0.927GlyCys: 0.927 ± 0.028
4.586GlyAsp: 4.586 ± 0.061
4.998GlyGlu: 4.998 ± 0.064
3.404GlyPhe: 3.404 ± 0.057
7.908GlyGly: 7.908 ± 0.111
1.794GlyHis: 1.794 ± 0.038
4.124GlyIle: 4.124 ± 0.055
3.311GlyLys: 3.311 ± 0.06
8.634GlyLeu: 8.634 ± 0.091
2.404GlyMet: 2.404 ± 0.043
2.206GlyAsn: 2.206 ± 0.04
3.69GlyPro: 3.69 ± 0.055
3.1GlyGln: 3.1 ± 0.05
6.226GlyArg: 6.226 ± 0.068
5.19GlySer: 5.19 ± 0.072
5.139GlyThr: 5.139 ± 0.08
6.4GlyVal: 6.4 ± 0.079
1.595GlyTrp: 1.595 ± 0.04
2.417GlyTyr: 2.417 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.617HisAla: 2.617 ± 0.042
0.254HisCys: 0.254 ± 0.015
1.237HisAsp: 1.237 ± 0.032
1.139HisGlu: 1.139 ± 0.029
0.84HisPhe: 0.84 ± 0.022
2.072HisGly: 2.072 ± 0.043
0.608HisHis: 0.608 ± 0.026
0.834HisIle: 0.834 ± 0.024
0.496HisLys: 0.496 ± 0.02
1.994HisLeu: 1.994 ± 0.044
0.451HisMet: 0.451 ± 0.02
0.473HisAsn: 0.473 ± 0.018
1.324HisPro: 1.324 ± 0.037
0.62HisGln: 0.62 ± 0.019
1.563HisArg: 1.563 ± 0.04
1.044HisSer: 1.044 ± 0.027
0.824HisThr: 0.824 ± 0.023
1.569HisVal: 1.569 ± 0.037
0.332HisTrp: 0.332 ± 0.017
0.567HisTyr: 0.567 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.129IleAla: 7.129 ± 0.08
0.474IleCys: 0.474 ± 0.018
3.626IleAsp: 3.626 ± 0.048
3.39IleGlu: 3.39 ± 0.044
1.252IlePhe: 1.252 ± 0.031
4.74IleGly: 4.74 ± 0.061
0.882IleHis: 0.882 ± 0.023
1.656IleIle: 1.656 ± 0.039
1.249IleLys: 1.249 ± 0.03
3.557IleLeu: 3.557 ± 0.062
0.879IleMet: 0.879 ± 0.027
1.301IleAsn: 1.301 ± 0.035
2.082IlePro: 2.082 ± 0.041
1.118IleGln: 1.118 ± 0.03
2.889IleArg: 2.889 ± 0.045
2.565IleSer: 2.565 ± 0.044
2.408IleThr: 2.408 ± 0.044
3.873IleVal: 3.873 ± 0.055
0.551IleTrp: 0.551 ± 0.023
0.992IleTyr: 0.992 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
3.951LysAla: 3.951 ± 0.064
0.179LysCys: 0.179 ± 0.012
1.49LysAsp: 1.49 ± 0.037
1.238LysGlu: 1.238 ± 0.037
0.855LysPhe: 0.855 ± 0.027
2.529LysGly: 2.529 ± 0.045
0.561LysHis: 0.561 ± 0.023
1.405LysIle: 1.405 ± 0.035
0.947LysLys: 0.947 ± 0.03
3.364LysLeu: 3.364 ± 0.055
0.7LysMet: 0.7 ± 0.024
0.678LysAsn: 0.678 ± 0.022
2.068LysPro: 2.068 ± 0.039
1.046LysGln: 1.046 ± 0.029
2.321LysArg: 2.321 ± 0.041
1.661LysSer: 1.661 ± 0.032
1.589LysThr: 1.589 ± 0.039
2.377LysVal: 2.377 ± 0.045
0.368LysTrp: 0.368 ± 0.018
0.613LysTyr: 0.613 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.532LeuAla: 14.532 ± 0.125
0.879LeuCys: 0.879 ± 0.023
6.028LeuAsp: 6.028 ± 0.07
4.996LeuGlu: 4.996 ± 0.061
3.371LeuPhe: 3.371 ± 0.054
8.591LeuGly: 8.591 ± 0.079
1.922LeuHis: 1.922 ± 0.04
4.372LeuIle: 4.372 ± 0.073
2.903LeuLys: 2.903 ± 0.05
9.484LeuLeu: 9.484 ± 0.114
2.062LeuMet: 2.062 ± 0.04
2.38LeuAsn: 2.38 ± 0.043
5.675LeuPro: 5.675 ± 0.068
2.935LeuGln: 2.935 ± 0.048
7.061LeuArg: 7.061 ± 0.077
6.079LeuSer: 6.079 ± 0.064
5.636LeuThr: 5.636 ± 0.064
7.541LeuVal: 7.541 ± 0.087
1.197LeuTrp: 1.197 ± 0.039
1.891LeuTyr: 1.891 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
3.133MetAla: 3.133 ± 0.049
0.172MetCys: 0.172 ± 0.011
1.132MetAsp: 1.132 ± 0.026
1.035MetGlu: 1.035 ± 0.029
0.686MetPhe: 0.686 ± 0.024
1.819MetGly: 1.819 ± 0.04
0.47MetHis: 0.47 ± 0.02
1.287MetIle: 1.287 ± 0.035
0.85MetLys: 0.85 ± 0.025
2.575MetLeu: 2.575 ± 0.051
0.619MetMet: 0.619 ± 0.023
0.684MetAsn: 0.684 ± 0.022
1.495MetPro: 1.495 ± 0.035
0.791MetGln: 0.791 ± 0.026
1.836MetArg: 1.836 ± 0.034
1.541MetSer: 1.541 ± 0.032
1.689MetThr: 1.689 ± 0.035
1.632MetVal: 1.632 ± 0.038
0.23MetTrp: 0.23 ± 0.013
0.287MetTyr: 0.287 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.292AsnAla: 3.292 ± 0.052
0.294AsnCys: 0.294 ± 0.016
1.355AsnAsp: 1.355 ± 0.028
1.137AsnGlu: 1.137 ± 0.033
0.938AsnPhe: 0.938 ± 0.031
2.396AsnGly: 2.396 ± 0.046
0.482AsnHis: 0.482 ± 0.019
1.1AsnIle: 1.1 ± 0.033
0.67AsnLys: 0.67 ± 0.024
2.554AsnLeu: 2.554 ± 0.051
0.537AsnMet: 0.537 ± 0.018
0.702AsnAsn: 0.702 ± 0.028
1.709AsnPro: 1.709 ± 0.039
0.753AsnGln: 0.753 ± 0.025
1.801AsnArg: 1.801 ± 0.039
1.264AsnSer: 1.264 ± 0.036
1.218AsnThr: 1.218 ± 0.034
1.894AsnVal: 1.894 ± 0.038
0.413AsnTrp: 0.413 ± 0.02
0.714AsnTyr: 0.714 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
6.939ProAla: 6.939 ± 0.091
0.396ProCys: 0.396 ± 0.017
3.469ProAsp: 3.469 ± 0.06
3.776ProGlu: 3.776 ± 0.054
1.862ProPhe: 1.862 ± 0.044
4.698ProGly: 4.698 ± 0.053
1.07ProHis: 1.07 ± 0.028
2.421ProIle: 2.421 ± 0.041
1.508ProLys: 1.508 ± 0.035
5.18ProLeu: 5.18 ± 0.067
1.192ProMet: 1.192 ± 0.034
1.314ProAsn: 1.314 ± 0.031
2.679ProPro: 2.679 ± 0.062
1.929ProGln: 1.929 ± 0.039
3.287ProArg: 3.287 ± 0.057
3.035ProSer: 3.035 ± 0.049
2.681ProThr: 2.681 ± 0.049
4.2ProVal: 4.2 ± 0.057
0.727ProTrp: 0.727 ± 0.023
1.085ProTyr: 1.085 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
4.689GlnAla: 4.689 ± 0.063
0.246GlnCys: 0.246 ± 0.011
1.717GlnAsp: 1.717 ± 0.036
1.491GlnGlu: 1.491 ± 0.034
1.148GlnPhe: 1.148 ± 0.028
2.772GlnGly: 2.772 ± 0.043
0.699GlnHis: 0.699 ± 0.025
1.705GlnIle: 1.705 ± 0.037
0.906GlnLys: 0.906 ± 0.029
3.397GlnLeu: 3.397 ± 0.049
0.84GlnMet: 0.84 ± 0.023
0.78GlnAsn: 0.78 ± 0.026
1.89GlnPro: 1.89 ± 0.044
1.271GlnGln: 1.271 ± 0.035
2.601GlnArg: 2.601 ± 0.051
1.797GlnSer: 1.797 ± 0.033
1.705GlnThr: 1.705 ± 0.038
2.755GlnVal: 2.755 ± 0.051
0.486GlnTrp: 0.486 ± 0.021
0.634GlnTyr: 0.634 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
8.392ArgAla: 8.392 ± 0.097
0.665ArgCys: 0.665 ± 0.025
4.083ArgAsp: 4.083 ± 0.051
4.332ArgGlu: 4.332 ± 0.058
3.023ArgPhe: 3.023 ± 0.051
5.115ArgGly: 5.115 ± 0.069
1.799ArgHis: 1.799 ± 0.04
3.88ArgIle: 3.88 ± 0.055
2.271ArgLys: 2.271 ± 0.038
7.966ArgLeu: 7.966 ± 0.088
1.975ArgMet: 1.975 ± 0.039
1.899ArgAsn: 1.899 ± 0.035
3.643ArgPro: 3.643 ± 0.051
2.663ArgGln: 2.663 ± 0.046
6.042ArgArg: 6.042 ± 0.089
4.133ArgSer: 4.133 ± 0.061
3.664ArgThr: 3.664 ± 0.051
4.995ArgVal: 4.995 ± 0.062
1.227ArgTrp: 1.227 ± 0.03
1.921ArgTyr: 1.921 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.819SerAla: 6.819 ± 0.08
0.465SerCys: 0.465 ± 0.017
3.088SerAsp: 3.088 ± 0.048
2.838SerGlu: 2.838 ± 0.048
2.12SerPhe: 2.12 ± 0.039
5.712SerGly: 5.712 ± 0.075
1.116SerHis: 1.116 ± 0.03
2.504SerIle: 2.504 ± 0.046
1.517SerLys: 1.517 ± 0.033
5.416SerLeu: 5.416 ± 0.067
1.346SerMet: 1.346 ± 0.032
1.473SerAsn: 1.473 ± 0.033
2.997SerPro: 2.997 ± 0.047
1.827SerGln: 1.827 ± 0.04
3.842SerArg: 3.842 ± 0.062
3.289SerSer: 3.289 ± 0.062
2.926SerThr: 2.926 ± 0.059
3.831SerVal: 3.831 ± 0.053
0.822SerTrp: 0.822 ± 0.026
1.468SerTyr: 1.468 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
6.324ThrAla: 6.324 ± 0.074
0.509ThrCys: 0.509 ± 0.017
2.739ThrAsp: 2.739 ± 0.05
2.333ThrGlu: 2.333 ± 0.043
1.934ThrPhe: 1.934 ± 0.035
5.38ThrGly: 5.38 ± 0.078
1.024ThrHis: 1.024 ± 0.029
2.876ThrIle: 2.876 ± 0.051
1.398ThrLys: 1.398 ± 0.034
5.647ThrLeu: 5.647 ± 0.066
1.272ThrMet: 1.272 ± 0.032
1.288ThrAsn: 1.288 ± 0.033
3.328ThrPro: 3.328 ± 0.057
1.685ThrGln: 1.685 ± 0.036
3.61ThrArg: 3.61 ± 0.053
3.055ThrSer: 3.055 ± 0.058
2.811ThrThr: 2.811 ± 0.053
4.033ThrVal: 4.033 ± 0.055
0.747ThrTrp: 0.747 ± 0.026
1.247ThrTyr: 1.247 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
9.424ValAla: 9.424 ± 0.095
0.629ValCys: 0.629 ± 0.02
4.141ValAsp: 4.141 ± 0.055
4.322ValGlu: 4.322 ± 0.05
2.355ValPhe: 2.355 ± 0.044
5.718ValGly: 5.718 ± 0.077
1.439ValHis: 1.439 ± 0.033
3.657ValIle: 3.657 ± 0.06
1.979ValLys: 1.979 ± 0.042
7.267ValLeu: 7.267 ± 0.073
1.611ValMet: 1.611 ± 0.036
2.0ValAsn: 2.0 ± 0.042
4.136ValPro: 4.136 ± 0.055
2.166ValGln: 2.166 ± 0.041
5.271ValArg: 5.271 ± 0.06
4.465ValSer: 4.465 ± 0.064
4.592ValThr: 4.592 ± 0.059
5.48ValVal: 5.48 ± 0.067
0.909ValTrp: 0.909 ± 0.025
1.4ValTyr: 1.4 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.368TrpAla: 1.368 ± 0.033
0.157TrpCys: 0.157 ± 0.009
0.711TrpAsp: 0.711 ± 0.026
0.627TrpGlu: 0.627 ± 0.024
0.585TrpPhe: 0.585 ± 0.021
0.923TrpGly: 0.923 ± 0.03
0.392TrpHis: 0.392 ± 0.016
0.674TrpIle: 0.674 ± 0.022
0.48TrpLys: 0.48 ± 0.02
1.804TrpLeu: 1.804 ± 0.047
0.379TrpMet: 0.379 ± 0.016
0.483TrpAsn: 0.483 ± 0.019
0.712TrpPro: 0.712 ± 0.022
0.648TrpGln: 0.648 ± 0.021
1.382TrpArg: 1.382 ± 0.032
0.952TrpSer: 0.952 ± 0.03
0.809TrpThr: 0.809 ± 0.026
0.856TrpVal: 0.856 ± 0.026
0.242TrpTrp: 0.242 ± 0.014
0.314TrpTyr: 0.314 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.682TyrAla: 2.682 ± 0.047
0.264TyrCys: 0.264 ± 0.015
1.488TyrAsp: 1.488 ± 0.032
1.241TyrGlu: 1.241 ± 0.03
0.829TyrPhe: 0.829 ± 0.025
2.116TyrGly: 2.116 ± 0.047
0.485TyrHis: 0.485 ± 0.019
0.813TyrIle: 0.813 ± 0.025
0.661TyrLys: 0.661 ± 0.026
2.148TyrLeu: 2.148 ± 0.048
0.414TyrMet: 0.414 ± 0.018
0.635TyrAsn: 0.635 ± 0.021
1.141TyrPro: 1.141 ± 0.031
0.744TyrGln: 0.744 ± 0.02
1.84TyrArg: 1.84 ± 0.039
1.257TyrSer: 1.257 ± 0.03
1.138TyrThr: 1.138 ± 0.033
1.572TyrVal: 1.572 ± 0.037
0.361TyrTrp: 0.361 ± 0.017
0.644TyrTyr: 0.644 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4782 proteins (1383869 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski