Burkholderia phage BcepGomr

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5A3R9|A5A3R9_9CAUD BcepGomrgp30 OS=Burkholderia phage BcepGomr OX=437329 GN=BcepGomrgene30 PE=4 SV=1
MM1 pKa = 8.15DD2 pKa = 6.18SIEE5 pKa = 4.83KK6 pKa = 10.47LYY8 pKa = 10.46QAYY11 pKa = 9.73LARR14 pKa = 11.84IASYY18 pKa = 11.31GEE20 pKa = 4.81GYY22 pKa = 8.72TPLDD26 pKa = 3.55FDD28 pKa = 4.91DD29 pKa = 3.67WRR31 pKa = 11.84EE32 pKa = 3.97AQDD35 pKa = 3.47PAVIEE40 pKa = 4.16LGEE43 pKa = 4.14PDD45 pKa = 4.17GYY47 pKa = 11.65LNAA50 pKa = 5.3

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5A3W0|A5A3W0_9CAUD BcepGomrgp71 OS=Burkholderia phage BcepGomr OX=437329 GN=BcepGomrgene71 PE=4 SV=1
MM1 pKa = 6.65RR2 pKa = 11.84TYY4 pKa = 11.01YY5 pKa = 10.39EE6 pKa = 4.36SEE8 pKa = 3.88QEE10 pKa = 4.57AIDD13 pKa = 3.81VAAARR18 pKa = 11.84YY19 pKa = 6.72GANWVANYY27 pKa = 9.36FLHH30 pKa = 7.36AFDD33 pKa = 5.15LPSGEE38 pKa = 4.25RR39 pKa = 11.84GWVILRR45 pKa = 11.84RR46 pKa = 11.84VEE48 pKa = 3.66KK49 pKa = 8.91TARR52 pKa = 11.84VAHH55 pKa = 6.47KK56 pKa = 9.78KK57 pKa = 9.83GKK59 pKa = 8.08VRR61 pKa = 11.84RR62 pKa = 11.84GPGQRR67 pKa = 11.84VQSKK71 pKa = 9.03IDD73 pKa = 3.54RR74 pKa = 11.84PVEE77 pKa = 3.49RR78 pKa = 11.84ARR80 pKa = 11.84AIFEE84 pKa = 4.2LLRR87 pKa = 11.84GKK89 pKa = 10.31GRR91 pKa = 11.84AEE93 pKa = 3.91VVQEE97 pKa = 4.27CVRR100 pKa = 11.84QGIAEE105 pKa = 4.28GTANTQYY112 pKa = 10.83SKK114 pKa = 10.29WMKK117 pKa = 10.51ASVVSDD123 pKa = 3.47TNGVNVSS130 pKa = 3.31

Molecular weight: 14.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

16416

40

1236

218.9

24.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.032 ± 0.478

1.078 ± 0.133

5.653 ± 0.166

6.037 ± 0.403

3.734 ± 0.146

8.096 ± 0.352

1.712 ± 0.175

5.434 ± 0.21

5.19 ± 0.358

6.926 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.973 ± 0.14

4.581 ± 0.285

4.983 ± 0.298

4.459 ± 0.308

5.574 ± 0.153

5.391 ± 0.194

5.556 ± 0.309

6.646 ± 0.228

1.864 ± 0.157

3.082 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski