Caenorhabditis japonica

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35026 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H2JLH8|A0A2H2JLH8_CAEJA Uncharacterized protein OS=Caenorhabditis japonica OX=281687 PE=4 SV=1
MM1 pKa = 7.56YY2 pKa = 10.48INDD5 pKa = 4.73LLEE8 pKa = 4.24MFPPDD13 pKa = 2.88IHH15 pKa = 5.67VTAFADD21 pKa = 3.85DD22 pKa = 4.4LKK24 pKa = 11.29LLSSNPASLQHH35 pKa = 6.55SINLVADD42 pKa = 4.13WFSILDD48 pKa = 3.91TANQAAPSYY57 pKa = 8.09TIPSDD62 pKa = 3.47SGPCSSCLEE71 pKa = 4.21YY72 pKa = 11.11ANSSAAVPEE81 pKa = 4.23VAPEE85 pKa = 3.87GLIVDD90 pKa = 4.05EE91 pKa = 5.21DD92 pKa = 4.06FDD94 pKa = 6.55RR95 pKa = 11.84ILDD98 pKa = 3.67VVGPSFRR105 pKa = 11.84RR106 pKa = 11.84TTAWSCC112 pKa = 3.12

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H2IB26|A0A2H2IB26_CAEJA Uncharacterized protein OS=Caenorhabditis japonica OX=281687 PE=4 SV=1
MM1 pKa = 7.46YY2 pKa = 10.32SRR4 pKa = 11.84SPPPRR9 pKa = 11.84GQLPDD14 pKa = 3.48SYY16 pKa = 11.18LAAVWRR22 pKa = 11.84LLGGSLAALWRR33 pKa = 11.84LFGGSLAALWRR44 pKa = 11.84LCVGYY49 pKa = 8.19RR50 pKa = 11.84TARR53 pKa = 11.84NRR55 pKa = 11.84TARR58 pKa = 11.84NRR60 pKa = 11.84TARR63 pKa = 11.84NRR65 pKa = 11.84TTRR68 pKa = 11.84NRR70 pKa = 11.84TARR73 pKa = 11.84NRR75 pKa = 11.84TTRR78 pKa = 11.84NRR80 pKa = 11.84TARR83 pKa = 11.84SRR85 pKa = 11.84TARR88 pKa = 11.84SRR90 pKa = 11.84TARR93 pKa = 11.84NRR95 pKa = 11.84TARR98 pKa = 11.84SRR100 pKa = 11.84TARR103 pKa = 11.84NRR105 pKa = 11.84TARR108 pKa = 11.84NRR110 pKa = 11.84TARR113 pKa = 11.84NRR115 pKa = 11.84TAQSRR120 pKa = 11.84TARR123 pKa = 11.84NRR125 pKa = 11.84TCRR128 pKa = 11.84NRR130 pKa = 11.84TARR133 pKa = 11.84NRR135 pKa = 11.84TARR138 pKa = 11.84NRR140 pKa = 11.84TARR143 pKa = 11.84NRR145 pKa = 11.84TAQSRR150 pKa = 11.84TARR153 pKa = 11.84NRR155 pKa = 11.84TAQSRR160 pKa = 11.84TARR163 pKa = 11.84NRR165 pKa = 11.84TARR168 pKa = 11.84DD169 pKa = 2.97RR170 pKa = 11.84TARR173 pKa = 11.84NRR175 pKa = 11.84TAQSRR180 pKa = 11.84TARR183 pKa = 11.84NRR185 pKa = 11.84TARR188 pKa = 11.84DD189 pKa = 2.91RR190 pKa = 11.84TTRR193 pKa = 11.84NRR195 pKa = 11.84TARR198 pKa = 11.84NRR200 pKa = 11.84TTRR203 pKa = 11.84NRR205 pKa = 11.84TARR208 pKa = 11.84SRR210 pKa = 11.84TARR213 pKa = 11.84SRR215 pKa = 11.84TARR218 pKa = 11.84SRR220 pKa = 11.84TARR223 pKa = 11.84SRR225 pKa = 11.84TARR228 pKa = 11.84NRR230 pKa = 11.84TARR233 pKa = 11.84NRR235 pKa = 11.84TAQSRR240 pKa = 11.84TARR243 pKa = 11.84NRR245 pKa = 11.84TVRR248 pKa = 11.84NRR250 pKa = 11.84TVRR253 pKa = 11.84NRR255 pKa = 11.84TARR258 pKa = 11.84NRR260 pKa = 11.84TARR263 pKa = 11.84NRR265 pKa = 11.84TARR268 pKa = 11.84NRR270 pKa = 11.84TTRR273 pKa = 11.84NRR275 pKa = 11.84TAQSRR280 pKa = 11.84TARR283 pKa = 11.84NRR285 pKa = 11.84TAQSRR290 pKa = 11.84TARR293 pKa = 11.84NRR295 pKa = 11.84TARR298 pKa = 11.84DD299 pKa = 2.91RR300 pKa = 11.84TTRR303 pKa = 11.84NRR305 pKa = 11.84TARR308 pKa = 11.84NRR310 pKa = 11.84TTRR313 pKa = 11.84NRR315 pKa = 11.84TARR318 pKa = 11.84SRR320 pKa = 11.84TARR323 pKa = 11.84SRR325 pKa = 11.84TSRR328 pKa = 11.84NRR330 pKa = 11.84TARR333 pKa = 11.84NRR335 pKa = 11.84TAQSRR340 pKa = 11.84TARR343 pKa = 11.84NRR345 pKa = 11.84TVRR348 pKa = 11.84NRR350 pKa = 11.84TVRR353 pKa = 11.84NRR355 pKa = 11.84TARR358 pKa = 11.84NRR360 pKa = 11.84TARR363 pKa = 11.84DD364 pKa = 2.91RR365 pKa = 11.84TTRR368 pKa = 11.84NRR370 pKa = 11.84TARR373 pKa = 11.84NRR375 pKa = 11.84TARR378 pKa = 11.84SRR380 pKa = 11.84TARR383 pKa = 11.84SRR385 pKa = 11.84TARR388 pKa = 11.84NRR390 pKa = 11.84TARR393 pKa = 11.84NRR395 pKa = 11.84TAQSRR400 pKa = 11.84TARR403 pKa = 11.84NRR405 pKa = 11.84TAQSRR410 pKa = 11.84TARR413 pKa = 11.84NRR415 pKa = 11.84TARR418 pKa = 11.84NRR420 pKa = 11.84TARR423 pKa = 11.84NRR425 pKa = 11.84TGSVSVQLLQSTINRR440 pKa = 11.84RR441 pKa = 11.84LIHH444 pKa = 6.54RR445 pKa = 11.84LINRR449 pKa = 11.84LNQSTINLVDD459 pKa = 3.26

Molecular weight: 53.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28999

6027

35026

10578374

20

9371

302.0

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.83 ± 0.016

1.936 ± 0.012

5.34 ± 0.014

6.787 ± 0.022

4.307 ± 0.012

5.428 ± 0.017

2.344 ± 0.008

5.563 ± 0.011

6.323 ± 0.018

8.517 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.007

4.648 ± 0.012

5.065 ± 0.017

4.12 ± 0.014

5.905 ± 0.019

7.96 ± 0.02

5.925 ± 0.016

6.356 ± 0.012

1.173 ± 0.005

2.87 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski