Mycobacterium sp. EPa45

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G3IPQ8|A0A0G3IPQ8_9MYCO Uncharacterized protein OS=Mycobacterium sp. EPa45 OX=1545728 GN=AB431_26080 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.5ASIDD6 pKa = 3.6DD7 pKa = 3.91GRR9 pKa = 11.84CRR11 pKa = 11.84GHH13 pKa = 6.4GVCTTVCPDD22 pKa = 3.31VFAMTDD28 pKa = 3.11DD29 pKa = 4.7GYY31 pKa = 11.86AEE33 pKa = 5.08AIVDD37 pKa = 3.93EE38 pKa = 4.91VPAGLEE44 pKa = 3.85DD45 pKa = 3.95SARR48 pKa = 11.84EE49 pKa = 4.0AADD52 pKa = 3.55ACPEE56 pKa = 3.92NAVILDD62 pKa = 3.55QQ63 pKa = 4.91

Molecular weight: 6.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G3IZX7|A0A0G3IZX7_9MYCO Long-chain fatty acid--CoA ligase OS=Mycobacterium sp. EPa45 OX=1545728 GN=AB431_23635 PE=4 SV=1
MM1 pKa = 7.19SQRR4 pKa = 11.84KK5 pKa = 8.86RR6 pKa = 11.84NRR8 pKa = 11.84GHH10 pKa = 6.78RR11 pKa = 11.84AAAGPIGSVAGSSLHH26 pKa = 6.78ADD28 pKa = 3.33PHH30 pKa = 7.09IVGPADD36 pKa = 3.3GSVWGRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84VLLLNSTYY52 pKa = 10.82EE53 pKa = 4.11PLTALPMRR61 pKa = 11.84RR62 pKa = 11.84AVIMLLCGKK71 pKa = 10.3ADD73 pKa = 3.61VVHH76 pKa = 7.73DD77 pKa = 4.4DD78 pKa = 3.46PGGPVIHH85 pKa = 6.67SATRR89 pKa = 11.84AIVVPSVIRR98 pKa = 11.84LRR100 pKa = 11.84SFVRR104 pKa = 11.84VPYY107 pKa = 10.02RR108 pKa = 11.84ARR110 pKa = 11.84IPMTRR115 pKa = 11.84AALMHH120 pKa = 6.99RR121 pKa = 11.84DD122 pKa = 3.56RR123 pKa = 11.84FRR125 pKa = 11.84CAYY128 pKa = 9.73CGAKK132 pKa = 9.99ADD134 pKa = 4.27TVDD137 pKa = 3.41HH138 pKa = 5.51VVPRR142 pKa = 11.84SRR144 pKa = 11.84GGEE147 pKa = 3.98HH148 pKa = 5.99SWEE151 pKa = 4.07NCVAACAPCNHH162 pKa = 6.7RR163 pKa = 11.84KK164 pKa = 9.54ADD166 pKa = 4.05RR167 pKa = 11.84LLTEE171 pKa = 4.92LGWTLRR177 pKa = 11.84LVPMPPKK184 pKa = 8.96GQHH187 pKa = 4.65WRR189 pKa = 11.84LLCTIKK195 pKa = 10.66EE196 pKa = 4.19LDD198 pKa = 4.05PAWVRR203 pKa = 11.84YY204 pKa = 9.55LGEE207 pKa = 4.14GAAA210 pKa = 4.63

Molecular weight: 23.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5595

0

5595

1821256

32

5191

325.5

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.837 ± 0.047

0.809 ± 0.009

6.309 ± 0.028

5.167 ± 0.025

3.117 ± 0.019

8.872 ± 0.031

2.191 ± 0.015

4.439 ± 0.022

2.264 ± 0.022

9.839 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.014

2.346 ± 0.019

5.787 ± 0.026

3.015 ± 0.018

6.978 ± 0.033

5.463 ± 0.022

6.076 ± 0.026

8.705 ± 0.032

1.518 ± 0.014

2.193 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski