Faeces associated gemycircularvirus 21

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus lamas1; Lama associated gemycircularvirus 1

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A161IBB7|A0A161IBB7_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 21 OX=1843741 PE=3 SV=1
MM1 pKa = 7.34TFAINAKK8 pKa = 9.8YY9 pKa = 10.82VLLTYY14 pKa = 9.11AQCGDD19 pKa = 3.65LDD21 pKa = 4.01GFAVMDD27 pKa = 5.51RR28 pKa = 11.84ISEE31 pKa = 4.19LGGEE35 pKa = 4.48CIVARR40 pKa = 11.84EE41 pKa = 3.98THH43 pKa = 6.64ADD45 pKa = 3.46GGTHH49 pKa = 6.5LHH51 pKa = 5.76VFCDD55 pKa = 4.88FGRR58 pKa = 11.84KK59 pKa = 8.16FRR61 pKa = 11.84SRR63 pKa = 11.84KK64 pKa = 7.62TDD66 pKa = 2.99VFDD69 pKa = 3.6VLGYY73 pKa = 10.25HH74 pKa = 6.77PNIEE78 pKa = 4.21PSRR81 pKa = 11.84GTPEE85 pKa = 3.59KK86 pKa = 10.76GYY88 pKa = 10.89DD89 pKa = 3.47YY90 pKa = 10.69AIKK93 pKa = 10.71DD94 pKa = 3.36GDD96 pKa = 4.31VICGGLARR104 pKa = 11.84PTTSRR109 pKa = 11.84TGDD112 pKa = 2.98SSANSKK118 pKa = 6.86WTEE121 pKa = 3.6ITSATNRR128 pKa = 11.84DD129 pKa = 3.88EE130 pKa = 4.4FWEE133 pKa = 4.4LVHH136 pKa = 8.01LLDD139 pKa = 5.09PKK141 pKa = 10.8SAACSFGQLQKK152 pKa = 10.46YY153 pKa = 9.47CDD155 pKa = 3.4WKK157 pKa = 10.91FAVAPPTYY165 pKa = 9.42TSPTGVEE172 pKa = 4.16FDD174 pKa = 4.78DD175 pKa = 5.19GSIDD179 pKa = 5.11GRR181 pKa = 11.84LDD183 pKa = 2.97WLQQSGVGSGQPLLGRR199 pKa = 11.84CMSLCLYY206 pKa = 10.73GEE208 pKa = 4.21SRR210 pKa = 11.84TGKK213 pKa = 7.35TLWARR218 pKa = 11.84SLGAHH223 pKa = 7.21IYY225 pKa = 10.48CVGLVSGDD233 pKa = 3.48EE234 pKa = 4.17CMKK237 pKa = 11.0ADD239 pKa = 4.21DD240 pKa = 4.31ADD242 pKa = 3.78YY243 pKa = 11.27AIFDD247 pKa = 5.27DD248 pKa = 3.68IRR250 pKa = 11.84GGIKK254 pKa = 9.99FFPSFKK260 pKa = 10.33EE261 pKa = 3.83WLGCQAWVTVKK272 pKa = 10.5CLYY275 pKa = 10.42RR276 pKa = 11.84EE277 pKa = 4.05PKK279 pKa = 9.36LIKK282 pKa = 9.2WGKK285 pKa = 7.39PSIWISNTDD294 pKa = 3.03PRR296 pKa = 11.84DD297 pKa = 3.62NMEE300 pKa = 4.08TSDD303 pKa = 3.87VHH305 pKa = 6.6WMNKK309 pKa = 8.26NCIFVAVDD317 pKa = 3.45SPIFRR322 pKa = 11.84ANTEE326 pKa = 3.84

Molecular weight:
36.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A161IBB7|A0A161IBB7_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 21 OX=1843741 PE=3 SV=1
MM1 pKa = 7.75AYY3 pKa = 10.21ARR5 pKa = 11.84RR6 pKa = 11.84SRR8 pKa = 11.84FPRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AFKK16 pKa = 10.3RR17 pKa = 11.84RR18 pKa = 11.84SAPRR22 pKa = 11.84RR23 pKa = 11.84SRR25 pKa = 11.84RR26 pKa = 11.84YY27 pKa = 7.59VAKK30 pKa = 10.17RR31 pKa = 11.84PSRR34 pKa = 11.84TRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84AMPSRR43 pKa = 11.84RR44 pKa = 11.84SILNLTTTKK53 pKa = 10.42KK54 pKa = 9.85RR55 pKa = 11.84DD56 pKa = 3.28NMAPAFYY63 pKa = 10.19RR64 pKa = 11.84AGDD67 pKa = 3.89GVTATPVNNGIALLATMPAYY87 pKa = 7.19TTAHH91 pKa = 5.96CVLWCATEE99 pKa = 4.17RR100 pKa = 11.84EE101 pKa = 4.42YY102 pKa = 11.52ASGSNMSDD110 pKa = 2.72HH111 pKa = 6.77GRR113 pKa = 11.84RR114 pKa = 11.84SHH116 pKa = 5.45TPFYY120 pKa = 10.51KK121 pKa = 10.64GLSEE125 pKa = 5.04RR126 pKa = 11.84IRR128 pKa = 11.84MKK130 pKa = 8.59TTNSSPWLWRR140 pKa = 11.84RR141 pKa = 11.84IVFTMKK147 pKa = 10.56GPLPEE152 pKa = 4.61TIRR155 pKa = 11.84TSIKK159 pKa = 10.38VGNQYY164 pKa = 10.67KK165 pKa = 10.44RR166 pKa = 11.84GITWLTDD173 pKa = 3.28GSDD176 pKa = 3.11VGNSVNGLVNTTLFAGSAGIDD197 pKa = 3.43WNDD200 pKa = 2.91VMLAKK205 pKa = 9.95TDD207 pKa = 3.8TQALTVMYY215 pKa = 10.45DD216 pKa = 2.99RR217 pKa = 11.84TIQLKK222 pKa = 10.35SGSDD226 pKa = 3.09SGTIRR231 pKa = 11.84DD232 pKa = 4.43FKK234 pKa = 10.45MCHH237 pKa = 6.06PMNKK241 pKa = 8.1TLVYY245 pKa = 10.28AYY247 pKa = 10.02DD248 pKa = 3.46EE249 pKa = 4.77SGTFDD254 pKa = 3.22VSFTRR259 pKa = 11.84SALGRR264 pKa = 11.84AGMGDD269 pKa = 3.44MYY271 pKa = 10.18IYY273 pKa = 11.32DD274 pKa = 4.12MFQPHH279 pKa = 7.26DD280 pKa = 3.85FSVDD284 pKa = 2.88GGTIEE289 pKa = 4.65FNPKK293 pKa = 8.25ATLYY297 pKa = 9.09WHH299 pKa = 6.97EE300 pKa = 4.28KK301 pKa = 9.3

Molecular weight:
34.19 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

860

233

326

286.7

31.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.093 ± 0.403

2.674 ± 0.793

7.442 ± 0.818

3.605 ± 0.604

4.419 ± 0.242

9.186 ± 0.924

2.093 ± 0.045

4.419 ± 0.367

5.0 ± 0.281

7.093 ± 0.786

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.969

3.023 ± 0.629

4.884 ± 0.404

1.977 ± 0.307

7.558 ± 1.701

7.674 ± 0.287

8.023 ± 0.929

5.349 ± 0.333

2.326 ± 0.345

3.605 ± 0.333

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski