Avian leukosis virus RSA (RSV-SRA) (Rous sarcoma virus (strain Schmidt-Ruppin A))

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Alpharetrovirus; Rous sarcoma virus; Rous sarcoma virus (strain Schmidt-Ruppin)

Average proteome isoelectric point is 7.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q04221|VP14_RSVSA Putative trans-acting factor OS=Avian leukosis virus RSA OX=269446 GN=trans-acting factor PE=4 SV=1
MM1 pKa = 7.24EE2 pKa = 5.23AVIKK6 pKa = 10.33VISSACKK13 pKa = 8.59TYY15 pKa = 10.41CGKK18 pKa = 10.33ISPSKK23 pKa = 10.7KK24 pKa = 9.94EE25 pKa = 3.54IGAMLSLLQKK35 pKa = 10.32EE36 pKa = 4.76GLLMSPSDD44 pKa = 4.13LYY46 pKa = 11.72SPGSWDD52 pKa = 4.68PITAALSQRR61 pKa = 11.84AMVLGKK67 pKa = 10.37SGEE70 pKa = 4.13LKK72 pKa = 9.42TWGLVLGALKK82 pKa = 10.28AARR85 pKa = 11.84EE86 pKa = 4.03EE87 pKa = 4.39QVTSEE92 pKa = 4.35QAKK95 pKa = 9.35FWLGLGGGRR104 pKa = 11.84VSPPGPEE111 pKa = 4.63CIEE114 pKa = 4.09KK115 pKa = 9.9PATEE119 pKa = 3.75RR120 pKa = 11.84RR121 pKa = 11.84IDD123 pKa = 3.28KK124 pKa = 10.81GEE126 pKa = 3.96EE127 pKa = 3.89VGEE130 pKa = 4.28TTAQRR135 pKa = 11.84DD136 pKa = 3.37AKK138 pKa = 9.74MAPEE142 pKa = 4.28KK143 pKa = 10.19MATPKK148 pKa = 10.17TVGTSCYY155 pKa = 10.07QCGTATGCNCATASAPPPPYY175 pKa = 9.97VGSGLYY181 pKa = 9.82PSLAGVGEE189 pKa = 4.04QQGQGGDD196 pKa = 3.96TPWGAEE202 pKa = 3.88QPRR205 pKa = 11.84AEE207 pKa = 4.76PGHH210 pKa = 6.9AGLAPGPALTDD221 pKa = 3.15WARR224 pKa = 11.84IRR226 pKa = 11.84EE227 pKa = 4.27EE228 pKa = 4.12LASTGPPVVAMPVVIKK244 pKa = 9.98TEE246 pKa = 4.41GPAWTPLEE254 pKa = 4.33PKK256 pKa = 10.46LITRR260 pKa = 11.84LADD263 pKa = 3.44TVRR266 pKa = 11.84TKK268 pKa = 11.01GLRR271 pKa = 11.84SPITMAEE278 pKa = 4.01VEE280 pKa = 4.65ALMSSPLLPHH290 pKa = 7.26DD291 pKa = 3.78VTNLMRR297 pKa = 11.84VILGPAPYY305 pKa = 9.99ALWMDD310 pKa = 3.45AWGVQLQTVIAAATRR325 pKa = 11.84DD326 pKa = 3.68PRR328 pKa = 11.84HH329 pKa = 6.07PANGQGRR336 pKa = 11.84GEE338 pKa = 4.12RR339 pKa = 11.84TNLDD343 pKa = 3.72RR344 pKa = 11.84LKK346 pKa = 11.02GLADD350 pKa = 3.43GMVGNPQGQAALLRR364 pKa = 11.84PGEE367 pKa = 4.17LVAITASALQAFRR380 pKa = 11.84EE381 pKa = 4.5VARR384 pKa = 11.84LAEE387 pKa = 4.33PAGPWADD394 pKa = 3.01ITQGPSEE401 pKa = 4.53SFVDD405 pKa = 4.25FANRR409 pKa = 11.84LIKK412 pKa = 10.44AVEE415 pKa = 4.43GSDD418 pKa = 3.86LPPSARR424 pKa = 11.84APVIIDD430 pKa = 3.54CFRR433 pKa = 11.84QKK435 pKa = 10.34SQPDD439 pKa = 3.21IQQLIRR445 pKa = 11.84AAPSTLTTPGEE456 pKa = 4.01IIKK459 pKa = 10.7YY460 pKa = 9.86VLDD463 pKa = 3.78RR464 pKa = 11.84QKK466 pKa = 10.46IAPLTDD472 pKa = 3.04QGIAAAMSSAIQPLVMAVVNRR493 pKa = 11.84EE494 pKa = 3.55RR495 pKa = 11.84DD496 pKa = 3.61GQTGSGGRR504 pKa = 11.84ARR506 pKa = 11.84GLCYY510 pKa = 9.87TCGSPGHH517 pKa = 5.93YY518 pKa = 9.31QAQCPKK524 pKa = 9.91KK525 pKa = 9.55RR526 pKa = 11.84KK527 pKa = 9.3SGNSRR532 pKa = 11.84EE533 pKa = 3.99RR534 pKa = 11.84CQLCDD539 pKa = 4.32GMGHH543 pKa = 6.08NAKK546 pKa = 9.54QCRR549 pKa = 11.84RR550 pKa = 11.84RR551 pKa = 11.84DD552 pKa = 3.51GNQGQRR558 pKa = 11.84PGKK561 pKa = 9.73GLSSGSWPVSEE572 pKa = 4.54QPAVSLAMTMEE583 pKa = 4.56HH584 pKa = 7.35KK585 pKa = 10.49DD586 pKa = 3.43RR587 pKa = 11.84PLVRR591 pKa = 11.84VILTNTGSHH600 pKa = 6.33PVKK603 pKa = 10.3QRR605 pKa = 11.84SVYY608 pKa = 8.6ITALLDD614 pKa = 3.34SGADD618 pKa = 2.97ITIISEE624 pKa = 4.22EE625 pKa = 4.3DD626 pKa = 3.45WPTDD630 pKa = 3.33WPVMEE635 pKa = 4.92AANPQIHH642 pKa = 6.88GIGGGIPMRR651 pKa = 11.84KK652 pKa = 8.45SRR654 pKa = 11.84DD655 pKa = 3.55MIEE658 pKa = 3.91VGVINRR664 pKa = 11.84DD665 pKa = 3.23GSLEE669 pKa = 4.04RR670 pKa = 11.84PLLLFPAVAMVRR682 pKa = 11.84GSILGRR688 pKa = 11.84DD689 pKa = 4.06CLQGLGLRR697 pKa = 11.84LTNLIGRR704 pKa = 11.84ATVLTVALHH713 pKa = 6.18LAIPLKK719 pKa = 9.84WKK721 pKa = 9.73PDD723 pKa = 3.61HH724 pKa = 6.02TPVWIDD730 pKa = 2.94QWPLPEE736 pKa = 4.87GKK738 pKa = 10.01LVALTQLVEE747 pKa = 4.57KK748 pKa = 9.81EE749 pKa = 4.09LQLGHH754 pKa = 6.94IEE756 pKa = 4.41PSLSCWNTPVFVIRR770 pKa = 11.84KK771 pKa = 8.86ASGSYY776 pKa = 10.32RR777 pKa = 11.84LLHH780 pKa = 7.0DD781 pKa = 4.61LRR783 pKa = 11.84AVNAKK788 pKa = 9.53LVPFGAVQQGAPVLSALPRR807 pKa = 11.84GWPLMVLDD815 pKa = 5.46LKK817 pKa = 11.31DD818 pKa = 4.03CFFSIPLAEE827 pKa = 4.19QDD829 pKa = 3.37RR830 pKa = 11.84EE831 pKa = 4.28AFAFTLPSVNNQAPARR847 pKa = 11.84RR848 pKa = 11.84FQWKK852 pKa = 9.35VLPQGMTCSPTICQLVVGQVLEE874 pKa = 4.13PLRR877 pKa = 11.84LKK879 pKa = 10.8HH880 pKa = 6.03PSLRR884 pKa = 11.84MLHH887 pKa = 6.24YY888 pKa = 10.21MDD890 pKa = 6.17DD891 pKa = 4.09LLLAASSHH899 pKa = 7.13DD900 pKa = 3.55GLEE903 pKa = 4.16AAGEE907 pKa = 4.24EE908 pKa = 4.56VISTLEE914 pKa = 3.75RR915 pKa = 11.84AGFTISPDD923 pKa = 3.32KK924 pKa = 10.49IQRR927 pKa = 11.84EE928 pKa = 4.07PGVQYY933 pKa = 10.87LGYY936 pKa = 10.0KK937 pKa = 10.18LGSTYY942 pKa = 10.3VAPVGLVAEE951 pKa = 4.42PRR953 pKa = 11.84IATLWDD959 pKa = 3.32VQKK962 pKa = 11.12LVGSLQWLRR971 pKa = 11.84PALGIPPRR979 pKa = 11.84LMGPFYY985 pKa = 10.43EE986 pKa = 4.39QLRR989 pKa = 11.84GSDD992 pKa = 3.66PNEE995 pKa = 3.45ARR997 pKa = 11.84EE998 pKa = 4.15WNLDD1002 pKa = 2.98MKK1004 pKa = 9.5MAWRR1008 pKa = 11.84EE1009 pKa = 3.71IVQLSTTAALEE1020 pKa = 4.09RR1021 pKa = 11.84WDD1023 pKa = 3.82PALPLEE1029 pKa = 4.65GAVARR1034 pKa = 11.84CEE1036 pKa = 3.77QGAIGVLGQGLSTHH1050 pKa = 6.51PRR1052 pKa = 11.84PCLWLFSTQPTKK1064 pKa = 11.17AFTAWLEE1071 pKa = 4.03VLTLLITKK1079 pKa = 9.91LRR1081 pKa = 11.84ASAVRR1086 pKa = 11.84TFGKK1090 pKa = 10.42EE1091 pKa = 3.51VDD1093 pKa = 3.67ILLLPACFRR1102 pKa = 11.84EE1103 pKa = 4.37DD1104 pKa = 3.38LPLPEE1109 pKa = 5.79GILLALRR1116 pKa = 11.84GFAGKK1121 pKa = 9.52IRR1123 pKa = 11.84SSDD1126 pKa = 3.42TPSIFDD1132 pKa = 3.27IARR1135 pKa = 11.84PLHH1138 pKa = 5.93VSLKK1142 pKa = 10.28VRR1144 pKa = 11.84VTDD1147 pKa = 3.89HH1148 pKa = 6.83PVPGPTAFTDD1158 pKa = 3.96ASSSTHH1164 pKa = 5.96KK1165 pKa = 10.92GVVVWRR1171 pKa = 11.84EE1172 pKa = 3.75GPRR1175 pKa = 11.84WEE1177 pKa = 4.22IKK1179 pKa = 10.37EE1180 pKa = 3.98IADD1183 pKa = 4.09LGASVQQLEE1192 pKa = 4.18ARR1194 pKa = 11.84AVAMALLLWPTTPTNVVTDD1213 pKa = 3.73SAFVAKK1219 pKa = 9.61MLLKK1223 pKa = 9.82MGQEE1227 pKa = 4.63GVPSTAAAFILEE1239 pKa = 4.5DD1240 pKa = 3.69ALSQRR1245 pKa = 11.84SAMAAVLHH1253 pKa = 5.73VRR1255 pKa = 11.84SHH1257 pKa = 7.02SEE1259 pKa = 3.69VPGFFTEE1266 pKa = 4.54GNDD1269 pKa = 3.71VADD1272 pKa = 3.87SQATFQAYY1280 pKa = 8.62PLRR1283 pKa = 11.84EE1284 pKa = 4.2AKK1286 pKa = 10.14DD1287 pKa = 3.34LHH1289 pKa = 5.98TALHH1293 pKa = 6.29IGPRR1297 pKa = 11.84ALSKK1301 pKa = 10.84ACNISMQQARR1311 pKa = 11.84EE1312 pKa = 4.09VVQTCPHH1319 pKa = 6.59CNSAPALEE1327 pKa = 4.92AGVNPRR1333 pKa = 11.84GLGPLQIWQTDD1344 pKa = 3.79FTLEE1348 pKa = 3.58PRR1350 pKa = 11.84MAPRR1354 pKa = 11.84SWLAVTVDD1362 pKa = 3.75TASSAIVVTQHH1373 pKa = 5.89GRR1375 pKa = 11.84VTSVAAQHH1383 pKa = 5.86HH1384 pKa = 6.5WATAIAVLGRR1394 pKa = 11.84PKK1396 pKa = 10.5AIKK1399 pKa = 9.02TDD1401 pKa = 3.34NGSCFTSKK1409 pKa = 8.57STRR1412 pKa = 11.84EE1413 pKa = 3.52WLARR1417 pKa = 11.84WGIAHH1422 pKa = 5.78TTGIPGNSQGQAMVEE1437 pKa = 3.89RR1438 pKa = 11.84ANRR1441 pKa = 11.84LLKK1444 pKa = 10.84DD1445 pKa = 3.88KK1446 pKa = 10.85IRR1448 pKa = 11.84VLAEE1452 pKa = 3.57GDD1454 pKa = 3.48GFMKK1458 pKa = 10.6RR1459 pKa = 11.84IPTSKK1464 pKa = 10.19QGEE1467 pKa = 4.36LLAKK1471 pKa = 10.45AMYY1474 pKa = 10.28ALNHH1478 pKa = 6.25FEE1480 pKa = 4.52RR1481 pKa = 11.84GEE1483 pKa = 4.08NTKK1486 pKa = 9.6TPIQKK1491 pKa = 9.21HH1492 pKa = 4.25WRR1494 pKa = 11.84PTVLTEE1500 pKa = 4.11GPPVKK1505 pKa = 10.12IRR1507 pKa = 11.84IEE1509 pKa = 3.85TGEE1512 pKa = 4.09WEE1514 pKa = 4.34KK1515 pKa = 11.25GWNVLVWGRR1524 pKa = 11.84GYY1526 pKa = 11.12AAVKK1530 pKa = 10.59NRR1532 pKa = 11.84DD1533 pKa = 3.13TDD1535 pKa = 3.61KK1536 pKa = 11.36VIWVPSRR1543 pKa = 11.84KK1544 pKa = 9.39VKK1546 pKa = 10.52PDD1548 pKa = 3.13VTQKK1552 pKa = 11.49DD1553 pKa = 3.91EE1554 pKa = 4.15VTKK1557 pKa = 10.61KK1558 pKa = 10.9DD1559 pKa = 3.32EE1560 pKa = 4.5ASPLFAGISDD1570 pKa = 4.51WIPWEE1575 pKa = 4.39DD1576 pKa = 3.79EE1577 pKa = 4.05QEE1579 pKa = 4.24GLQGEE1584 pKa = 4.55TASNKK1589 pKa = 7.35QEE1591 pKa = 4.17RR1592 pKa = 11.84PGEE1595 pKa = 4.05DD1596 pKa = 2.5TLAANEE1602 pKa = 4.27SS1603 pKa = 3.7

Molecular weight: 173.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q04221|VP14_RSVSA Putative trans-acting factor OS=Avian leukosis virus RSA OX=269446 GN=trans-acting factor PE=4 SV=1
MM1 pKa = 7.24EE2 pKa = 4.52AVIRR6 pKa = 11.84EE7 pKa = 4.53GGSLPQVRR15 pKa = 11.84SASRR19 pKa = 11.84NQQRR23 pKa = 11.84SGEE26 pKa = 4.3STKK29 pKa = 10.22GRR31 pKa = 11.84KK32 pKa = 7.66WEE34 pKa = 3.93KK35 pKa = 9.27QLRR38 pKa = 11.84SEE40 pKa = 3.86MRR42 pKa = 11.84RR43 pKa = 11.84WRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 8.69WPHH51 pKa = 6.44LKK53 pKa = 10.13PLAHH57 pKa = 6.81PAISAEE63 pKa = 3.93QLLAVIAPQPRR74 pKa = 11.84PLLLLMWGVVCILPWRR90 pKa = 11.84GWEE93 pKa = 3.84SSRR96 pKa = 11.84ARR98 pKa = 11.84GVTHH102 pKa = 7.13LGGRR106 pKa = 11.84NSQGRR111 pKa = 11.84SQGTRR116 pKa = 11.84VWPLGRR122 pKa = 11.84PP123 pKa = 3.5

Molecular weight: 14.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3033

123

1603

758.3

82.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.298 ± 1.032

2.176 ± 0.482

4.088 ± 0.34

5.407 ± 0.394

1.912 ± 0.474

9.1 ± 0.547

1.78 ± 0.209

4.946 ± 0.251

4.715 ± 0.163

10.122 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.302

2.44 ± 0.399

7.451 ± 0.552

4.748 ± 0.209

6.528 ± 0.768

6.759 ± 0.506

6.067 ± 0.336

6.429 ± 0.294

2.374 ± 0.301

1.385 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski