Colletotrichum sublineola (Sorghum anthracnose fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A066XKW2|A0A066XKW2_COLSU Putative GDSL-like Lipase/Acylhydrolase OS=Colletotrichum sublineola OX=1173701 GN=CSUB01_11153 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84FSAYY6 pKa = 9.84FLAASLSASTAVAAPISYY24 pKa = 8.93YY25 pKa = 10.36VWSITNFSFDD35 pKa = 3.8AGSQSVSFQVSGPTGEE51 pKa = 4.59YY52 pKa = 10.7DD53 pKa = 5.62DD54 pKa = 5.14IPIPAFNLTGPCVVKK69 pKa = 10.95NDD71 pKa = 4.39GSTTDD76 pKa = 3.62CSALIEE82 pKa = 4.69NNTSGQTLKK91 pKa = 10.81SGPLTIDD98 pKa = 3.05NDD100 pKa = 3.88AGTVTSPTIFGFSSGGTSLEE120 pKa = 3.87VSTDD124 pKa = 4.01FFSKK128 pKa = 9.94TDD130 pKa = 3.5GPSTFTIDD138 pKa = 4.69GEE140 pKa = 4.47RR141 pKa = 11.84LNQAA145 pKa = 3.71

Molecular weight: 15.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A066XMB8|A0A066XMB8_COLSU Uncharacterized protein OS=Colletotrichum sublineola OX=1173701 GN=CSUB01_03917 PE=3 SV=1
MM1 pKa = 7.85PLRR4 pKa = 11.84STRR7 pKa = 11.84HH8 pKa = 3.33TTAAPRR14 pKa = 11.84RR15 pKa = 11.84TGLFSRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84AAPHH27 pKa = 5.56HH28 pKa = 6.09HH29 pKa = 5.58TTTRR33 pKa = 11.84TTHH36 pKa = 4.94TTTTTKK42 pKa = 10.49RR43 pKa = 11.84GGLFSRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84GPVHH55 pKa = 6.11TAPVHH60 pKa = 4.29HH61 pKa = 6.98HH62 pKa = 5.83RR63 pKa = 11.84RR64 pKa = 11.84KK65 pKa = 9.97PSMGDD70 pKa = 3.3KK71 pKa = 10.53ISGAMLKK78 pKa = 10.67LKK80 pKa = 10.43GALTRR85 pKa = 11.84RR86 pKa = 11.84PGQKK90 pKa = 9.9AAGTRR95 pKa = 11.84RR96 pKa = 11.84MHH98 pKa = 5.68GTDD101 pKa = 2.9GRR103 pKa = 11.84GSRR106 pKa = 11.84RR107 pKa = 11.84YY108 pKa = 9.57

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12697

0

12697

6235361

13

12854

491.1

54.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.19 ± 0.023

1.237 ± 0.008

5.777 ± 0.016

6.0 ± 0.027

3.666 ± 0.014

7.103 ± 0.024

2.37 ± 0.01

4.618 ± 0.014

4.724 ± 0.021

8.702 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.008

3.612 ± 0.012

6.258 ± 0.026

3.952 ± 0.015

6.195 ± 0.023

7.978 ± 0.027

5.962 ± 0.025

6.295 ± 0.02

1.488 ± 0.007

2.664 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski