Rhizobium deserti

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4410 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5UKE4|A0A4R5UKE4_9RHIZ Phosphoadenylyl-sulfate reductase OS=Rhizobium deserti OX=2547961 GN=E2F50_10965 PE=3 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAAVSGAQAADD24 pKa = 4.21AIVAAEE30 pKa = 4.47PEE32 pKa = 3.93PMEE35 pKa = 4.1YY36 pKa = 10.8VRR38 pKa = 11.84VCDD41 pKa = 4.28AFGKK45 pKa = 10.52GYY47 pKa = 10.22FYY49 pKa = 10.67IPGTEE54 pKa = 3.83TCLKK58 pKa = 9.33IGGYY62 pKa = 9.13VRR64 pKa = 11.84FQVDD68 pKa = 3.91FDD70 pKa = 4.56DD71 pKa = 4.53VDD73 pKa = 4.1DD74 pKa = 4.47VVAGAGGAVIDD85 pKa = 5.24IDD87 pKa = 4.46DD88 pKa = 5.01DD89 pKa = 3.52NWNARR94 pKa = 11.84TRR96 pKa = 11.84ALINVSAKK104 pKa = 10.52SDD106 pKa = 3.62TEE108 pKa = 4.06YY109 pKa = 11.28GALSSYY115 pKa = 10.36IALRR119 pKa = 11.84SWAEE123 pKa = 3.24GDD125 pKa = 3.81YY126 pKa = 11.34VGDD129 pKa = 4.44GEE131 pKa = 5.36GEE133 pKa = 4.17LNEE136 pKa = 4.58LEE138 pKa = 4.18IDD140 pKa = 3.51EE141 pKa = 5.67AYY143 pKa = 8.4ITLGGFKK150 pKa = 10.22VGYY153 pKa = 9.88AYY155 pKa = 10.66NYY157 pKa = 9.3WDD159 pKa = 3.79IGLSGEE165 pKa = 4.16TDD167 pKa = 3.34DD168 pKa = 6.02LGSNRR173 pKa = 11.84INQIGYY179 pKa = 8.96EE180 pKa = 4.17YY181 pKa = 10.42TSEE184 pKa = 4.19TIKK187 pKa = 11.05AGIFVDD193 pKa = 4.33EE194 pKa = 4.42LTGHH198 pKa = 4.91YY199 pKa = 9.35TEE201 pKa = 5.19NDD203 pKa = 2.89FGAALGTTDD212 pKa = 4.54GYY214 pKa = 11.84GNDD217 pKa = 3.91GVGIEE222 pKa = 4.5AMVSGAFGPISASLLGGYY240 pKa = 10.02DD241 pKa = 3.96FAAEE245 pKa = 4.43DD246 pKa = 4.02GSIRR250 pKa = 11.84GIVSGKK256 pKa = 10.03IGTGTLSVAGIYY268 pKa = 8.61STGANAYY275 pKa = 9.77YY276 pKa = 10.69DD277 pKa = 3.79LAEE280 pKa = 4.0WTVAAEE286 pKa = 4.18YY287 pKa = 10.43AVKK290 pKa = 10.68LGDD293 pKa = 4.19KK294 pKa = 9.13FTITPAFQYY303 pKa = 9.99WDD305 pKa = 3.96SLDD308 pKa = 4.07ADD310 pKa = 4.05LSGDD314 pKa = 3.57FGGDD318 pKa = 2.89RR319 pKa = 11.84NAWRR323 pKa = 11.84AGVTLDD329 pKa = 3.47YY330 pKa = 10.82KK331 pKa = 10.57IVEE334 pKa = 4.37GLTSKK339 pKa = 10.85VSLQYY344 pKa = 11.15TDD346 pKa = 4.05MDD348 pKa = 5.08DD349 pKa = 5.7DD350 pKa = 5.58GDD352 pKa = 3.77ISDD355 pKa = 4.72DD356 pKa = 3.84EE357 pKa = 4.6EE358 pKa = 4.61VFSGFVRR365 pKa = 11.84LQRR368 pKa = 11.84TFF370 pKa = 3.08

Molecular weight: 39.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5UHC2|A0A4R5UHC2_9RHIZ Transporter substrate-binding domain-containing protein OS=Rhizobium deserti OX=2547961 GN=E2F50_13420 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATTGGRR28 pKa = 11.84KK29 pKa = 9.01VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.99

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4410

0

4410

1370632

17

2834

310.8

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.774 ± 0.055

0.761 ± 0.011

5.688 ± 0.028

5.899 ± 0.033

3.879 ± 0.024

8.269 ± 0.031

1.993 ± 0.016

5.595 ± 0.03

3.743 ± 0.025

10.034 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.647 ± 0.021

2.798 ± 0.021

4.883 ± 0.028

3.355 ± 0.025

6.627 ± 0.031

5.995 ± 0.03

5.228 ± 0.024

7.315 ± 0.03

1.248 ± 0.013

2.271 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski