Candidatus Electronema sp. GS

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Candidatus Electronema; unclassified Candidatus Electronema

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A521G4F0|A0A521G4F0_9DELT Diadenylate cyclase OS=Candidatus Electronema sp. GS OX=2005002 GN=dacA PE=3 SV=1
MM1 pKa = 7.05QNSVQTITVLTAALLLGATAASALPIKK28 pKa = 10.53SGNDD32 pKa = 2.94KK33 pKa = 10.98VQVQLYY39 pKa = 9.8GEE41 pKa = 4.11VDD43 pKa = 3.61RR44 pKa = 11.84AVMYY48 pKa = 10.96ADD50 pKa = 5.55DD51 pKa = 5.44GYY53 pKa = 11.09QDD55 pKa = 4.9KK56 pKa = 10.74IFHH59 pKa = 6.54VDD61 pKa = 3.1NTHH64 pKa = 5.68SEE66 pKa = 4.27TRR68 pKa = 11.84VGLNGEE74 pKa = 4.29VTATQCLTVGGNVEE88 pKa = 4.9LKK90 pKa = 10.14WEE92 pKa = 4.47ANPARR97 pKa = 11.84AVSMDD102 pKa = 3.53EE103 pKa = 3.92EE104 pKa = 4.82SISGEE109 pKa = 3.82FAAEE113 pKa = 4.24LIEE116 pKa = 4.52MYY118 pKa = 10.61INTANAGKK126 pKa = 10.4LSLGQGSTASDD137 pKa = 3.19ASSEE141 pKa = 4.06VDD143 pKa = 4.44LSGTDD148 pKa = 3.29IIGNAGVADD157 pKa = 4.51LGGGLSFYY165 pKa = 10.98DD166 pKa = 3.76AAVDD170 pKa = 4.84GYY172 pKa = 11.84SDD174 pKa = 3.76LTADD178 pKa = 3.7AVFHH182 pKa = 6.31GGISAEE188 pKa = 4.08GLGKK192 pKa = 10.07INRR195 pKa = 11.84VLYY198 pKa = 6.59EE199 pKa = 3.83TRR201 pKa = 11.84SFGGFTFGASAGEE214 pKa = 4.1EE215 pKa = 4.11EE216 pKa = 4.85VVDD219 pKa = 4.51FALSYY224 pKa = 11.0AGEE227 pKa = 4.2FNGNQLEE234 pKa = 4.69AKK236 pKa = 9.74AAWSNPGEE244 pKa = 4.75GYY246 pKa = 10.07NQINGSASLLLNSGLNFTVAASSKK270 pKa = 10.95DD271 pKa = 3.68VDD273 pKa = 3.57NMPAYY278 pKa = 10.41GDD280 pKa = 4.73DD281 pKa = 3.54PSFMYY286 pKa = 10.41GKK288 pKa = 9.72IGYY291 pKa = 8.9KK292 pKa = 9.83CDD294 pKa = 3.47QLSSFGSTAVSVDD307 pKa = 3.16YY308 pKa = 11.25GIFNNADD315 pKa = 3.67LLDD318 pKa = 4.5AEE320 pKa = 4.72QEE322 pKa = 3.93ATAIGVQFVQEE333 pKa = 4.02LSDD336 pKa = 3.94YY337 pKa = 9.68STQLYY342 pKa = 9.63AGYY345 pKa = 10.03RR346 pKa = 11.84AFSLEE351 pKa = 4.34DD352 pKa = 3.34NTGVDD357 pKa = 3.82YY358 pKa = 11.5EE359 pKa = 4.87DD360 pKa = 4.27ISVVMAGARR369 pKa = 11.84LSFF372 pKa = 4.08

Molecular weight: 39.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A521G0Z2|A0A521G0Z2_9DELT Uncharacterized protein OS=Candidatus Electronema sp. GS OX=2005002 GN=CDV28_1196 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.48NIDD5 pKa = 3.65RR6 pKa = 11.84RR7 pKa = 11.84GVLPDD12 pKa = 3.34GLRR15 pKa = 11.84LPRR18 pKa = 11.84CPRR21 pKa = 11.84QSAKK25 pKa = 10.21IGCTRR30 pKa = 11.84VQRR33 pKa = 11.84WILLSCNGGYY43 pKa = 10.06FEE45 pKa = 4.66STTDD49 pKa = 3.02RR50 pKa = 11.84FTRR53 pKa = 11.84VQWLLSLSCKK63 pKa = 10.02LRR65 pKa = 11.84ADD67 pKa = 4.06YY68 pKa = 9.61PCRR71 pKa = 11.84AARR74 pKa = 11.84SGVRR78 pKa = 11.84PNAPAPSRR86 pKa = 11.84HH87 pKa = 5.96CRR89 pKa = 11.84GDD91 pKa = 3.13PCDD94 pKa = 4.56RR95 pKa = 11.84PMCRR99 pKa = 11.84HH100 pKa = 5.92IATMRR105 pKa = 11.84GEE107 pKa = 4.29YY108 pKa = 9.94KK109 pKa = 9.78IRR111 pKa = 11.84PYY113 pKa = 10.39RR114 pKa = 11.84PCRR117 pKa = 11.84AACNCAKK124 pKa = 10.4PLRR127 pKa = 11.84HH128 pKa = 5.65VSKK131 pKa = 10.11EE132 pKa = 3.91GWPMTSIICTAWLTCSSAGARR153 pKa = 11.84LHH155 pKa = 6.84RR156 pKa = 11.84SSS158 pKa = 3.93

Molecular weight: 17.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2614

0

2614

805550

28

2264

308.2

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.478 ± 0.055

1.432 ± 0.022

5.074 ± 0.034

6.76 ± 0.055

4.1 ± 0.039

7.163 ± 0.043

2.125 ± 0.022

6.141 ± 0.043

5.398 ± 0.052

10.627 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.021

3.553 ± 0.031

4.326 ± 0.031

4.24 ± 0.042

5.656 ± 0.042

6.042 ± 0.044

4.964 ± 0.043

6.592 ± 0.036

1.144 ± 0.02

2.744 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski