Blackberry leaf mottle associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus; unclassified Emaravirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5RWG7|A0A1W5RWG7_9VIRU Nucleocapsid protein OS=Blackberry leaf mottle associated virus OX=1966378 GN=P3 PE=4 SV=1
MM1 pKa = 7.06EE2 pKa = 6.01AKK4 pKa = 10.56AFDD7 pKa = 4.16YY8 pKa = 10.28TFHH11 pKa = 6.95SCTKK15 pKa = 9.55GGIMKK20 pKa = 10.39YY21 pKa = 9.99YY22 pKa = 10.85DD23 pKa = 4.4EE24 pKa = 6.3IDD26 pKa = 3.48FQLFFGFLEE35 pKa = 4.07EE36 pKa = 5.24LKK38 pKa = 10.99DD39 pKa = 4.29CIIYY43 pKa = 10.63DD44 pKa = 3.7HH45 pKa = 6.74LNKK48 pKa = 10.06LRR50 pKa = 11.84EE51 pKa = 4.1LAHH54 pKa = 6.75GDD56 pKa = 3.78LDD58 pKa = 4.33VSDD61 pKa = 4.42VDD63 pKa = 4.02GKK65 pKa = 9.53KK66 pKa = 8.15TAIQLTPDD74 pKa = 3.46FQTNQKK80 pKa = 10.14VMMYY84 pKa = 9.07FLRR87 pKa = 11.84YY88 pKa = 8.36HH89 pKa = 6.06QLLASLTGMKK99 pKa = 10.03PKK101 pKa = 10.69FIRR104 pKa = 11.84NIHH107 pKa = 6.31HH108 pKa = 7.34DD109 pKa = 3.23FHH111 pKa = 9.26IFGTLVRR118 pKa = 11.84GNEE121 pKa = 4.18MFPYY125 pKa = 10.14IPDD128 pKa = 3.49NMRR131 pKa = 11.84CLLLRR136 pKa = 11.84SDD138 pKa = 3.75ILKK141 pKa = 8.21TAVYY145 pKa = 9.8QVVKK149 pKa = 10.83GKK151 pKa = 10.76DD152 pKa = 3.09PMAALNMANGRR163 pKa = 11.84QPKK166 pKa = 9.19GFSYY170 pKa = 10.85KK171 pKa = 10.17EE172 pKa = 3.81QTSSYY177 pKa = 8.36TFQVIAISYY186 pKa = 9.06IALKK190 pKa = 10.59SSTEE194 pKa = 3.89IPGDD198 pKa = 3.43EE199 pKa = 4.31CFVPRR204 pKa = 11.84LVQKK208 pKa = 11.01SPDD211 pKa = 3.64VNDD214 pKa = 4.7DD215 pKa = 3.48EE216 pKa = 6.18SSGDD220 pKa = 3.58EE221 pKa = 4.13LNIIVV226 pKa = 4.35

Molecular weight: 25.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5RYJ7|A0A1W5RYJ7_9VIRU Movement protein OS=Blackberry leaf mottle associated virus OX=1966378 GN=P4 PE=4 SV=1
MM1 pKa = 7.63PPKK4 pKa = 9.65TDD6 pKa = 3.28RR7 pKa = 11.84SASKK11 pKa = 9.16ATSSTPPSLVPANSLLVGSGTKK33 pKa = 10.26LRR35 pKa = 11.84TVKK38 pKa = 10.67LNNVVNKK45 pKa = 10.07VLLNPEE51 pKa = 3.93TSEE54 pKa = 3.99LKK56 pKa = 10.42PRR58 pKa = 11.84AKK60 pKa = 10.7DD61 pKa = 3.33PVPFTSASQHH71 pKa = 4.12QTNFTLTKK79 pKa = 10.36YY80 pKa = 10.39RR81 pKa = 11.84DD82 pKa = 3.76YY83 pKa = 11.85CNLTTVASYY92 pKa = 10.8LSRR95 pKa = 11.84SRR97 pKa = 11.84EE98 pKa = 4.11LKK100 pKa = 10.9DD101 pKa = 3.5EE102 pKa = 4.21LQKK105 pKa = 11.53SNLVLTTAAGKK116 pKa = 8.35TLTIVKK122 pKa = 9.67DD123 pKa = 3.93LNDD126 pKa = 3.72SDD128 pKa = 4.38VEE130 pKa = 4.32NVVSFNKK137 pKa = 10.18ACAIMSAGILKK148 pKa = 8.87HH149 pKa = 5.6TFLEE153 pKa = 4.51VFDD156 pKa = 4.05WTKK159 pKa = 10.97KK160 pKa = 10.27EE161 pKa = 4.01YY162 pKa = 10.92VQTTVEE168 pKa = 4.41GRR170 pKa = 11.84SAPDD174 pKa = 2.8ITIINKK180 pKa = 9.36LAGAMGLQPGNPYY193 pKa = 10.41YY194 pKa = 10.44WMIVPGYY201 pKa = 8.6EE202 pKa = 4.21FLYY205 pKa = 10.04EE206 pKa = 4.26LYY208 pKa = 9.64PAEE211 pKa = 4.09VLAYY215 pKa = 8.76TLVRR219 pKa = 11.84LEE221 pKa = 5.04FRR223 pKa = 11.84SQLNIPTDD231 pKa = 3.51MTDD234 pKa = 3.15ADD236 pKa = 4.48IISSLVMKK244 pKa = 9.26MNRR247 pKa = 11.84IHH249 pKa = 7.11ALEE252 pKa = 4.09STSFDD257 pKa = 3.28EE258 pKa = 5.57AINIVGEE265 pKa = 4.07DD266 pKa = 3.89NIRR269 pKa = 11.84EE270 pKa = 4.26VYY272 pKa = 10.68LEE274 pKa = 3.9LARR277 pKa = 11.84DD278 pKa = 3.45VGTTSKK284 pKa = 9.24TRR286 pKa = 11.84RR287 pKa = 11.84NDD289 pKa = 3.12EE290 pKa = 4.77AILKK294 pKa = 8.57FKK296 pKa = 10.81QLITNFGAALHH307 pKa = 5.71EE308 pKa = 4.17EE309 pKa = 4.46RR310 pKa = 11.84VRR312 pKa = 11.84AGHH315 pKa = 6.66AA316 pKa = 3.13

Molecular weight: 35.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3859

226

2302

771.8

89.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.328 ± 0.624

1.71 ± 0.462

6.815 ± 0.624

5.649 ± 0.176

4.276 ± 0.231

3.421 ± 0.682

2.565 ± 0.187

8.526 ± 0.748

7.955 ± 0.197

9.692 ± 0.47

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.954 ± 0.438

6.867 ± 0.851

3.472 ± 0.221

2.799 ± 0.403

3.783 ± 0.388

7.1 ± 0.178

6.09 ± 0.513

5.96 ± 0.694

0.466 ± 0.128

5.571 ± 0.532
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski