Vagococcus salmoninarum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A429ZV08|A0A429ZV08_9ENTE QacE family quaternary ammonium compound efflux SMR transporter OS=Vagococcus salmoninarum OX=2739 GN=CBF35_02435 PE=3 SV=1
MM1 pKa = 7.04NVKK4 pKa = 9.08TKK6 pKa = 10.78LVGIVAIATTTMGFGATAFADD27 pKa = 3.52SIYY30 pKa = 9.89TVKK33 pKa = 10.85SGDD36 pKa = 3.62TLSQISYY43 pKa = 10.16DD44 pKa = 3.94YY45 pKa = 11.04LGSADD50 pKa = 3.82QYY52 pKa = 8.81TTIAQSNNILNADD65 pKa = 4.97LIYY68 pKa = 10.83VGQKK72 pKa = 10.49LKK74 pKa = 11.08LSADD78 pKa = 3.84GQISEE83 pKa = 4.27ATADD87 pKa = 3.52EE88 pKa = 4.39VATIEE93 pKa = 4.25EE94 pKa = 4.39ATTPVIEE101 pKa = 4.4EE102 pKa = 3.86NTPVVEE108 pKa = 4.15EE109 pKa = 4.34TPAVVEE115 pKa = 4.14EE116 pKa = 4.57APVAEE121 pKa = 4.32PVQEE125 pKa = 4.12VEE127 pKa = 4.48QVQEE131 pKa = 3.98SAPAGRR137 pKa = 11.84TITVEE142 pKa = 3.76TTAYY146 pKa = 10.61DD147 pKa = 4.63GISLGGLTATGYY159 pKa = 10.79QITGYY164 pKa = 8.82GDD166 pKa = 3.15KK167 pKa = 10.92VIAVDD172 pKa = 4.11PNVIPLGSTVYY183 pKa = 10.49VPGYY187 pKa = 8.3GTAIAADD194 pKa = 3.65TGGAIQGNIIDD205 pKa = 5.24LNMSTADD212 pKa = 3.85AIQWGRR218 pKa = 11.84RR219 pKa = 11.84SVTITILL226 pKa = 3.4

Molecular weight: 23.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A429ZIJ5|A0A429ZIJ5_9ENTE Aldo/keto reductase OS=Vagococcus salmoninarum OX=2739 GN=CBF35_11335 PE=4 SV=1
MM1 pKa = 7.13AQQRR5 pKa = 11.84RR6 pKa = 11.84GGKK9 pKa = 8.91RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.35VCYY16 pKa = 9.34FCANHH21 pKa = 6.06VDD23 pKa = 3.4HH24 pKa = 7.29VDD26 pKa = 3.68YY27 pKa = 11.34KK28 pKa = 11.16DD29 pKa = 3.67VEE31 pKa = 4.32LLKK34 pKa = 11.01SRR36 pKa = 11.84FISEE40 pKa = 3.98RR41 pKa = 11.84GKK43 pKa = 9.78ILPRR47 pKa = 11.84RR48 pKa = 11.84VTGTCAKK55 pKa = 9.69HH56 pKa = 5.69QRR58 pKa = 11.84TLTVAIKK65 pKa = 10.14RR66 pKa = 11.84ARR68 pKa = 11.84IMGLLPFVGEE78 pKa = 4.26EE79 pKa = 3.86QQ80 pKa = 3.46

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2859

0

2859

899546

20

2415

314.6

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.202 ± 0.05

0.565 ± 0.012

4.993 ± 0.035

7.33 ± 0.052

4.397 ± 0.036

6.766 ± 0.045

1.698 ± 0.02

7.555 ± 0.039

6.782 ± 0.04

10.495 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.025

4.573 ± 0.031

3.51 ± 0.027

4.258 ± 0.036

3.479 ± 0.033

6.272 ± 0.041

6.14 ± 0.041

6.972 ± 0.036

0.858 ± 0.015

3.586 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski