Erysipelothrix sp. HDW6B

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelothrix; unclassified Erysipelothrix

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7ZCT9|A0A6G7ZCT9_9FIRM Uncharacterized protein OS=Erysipelothrix sp. HDW6B OX=2714929 GN=G7061_09225 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.35KK3 pKa = 10.5SIISVLLVTLLLFVSGCTQAGDD25 pKa = 4.05GYY27 pKa = 11.19KK28 pKa = 9.99VARR31 pKa = 11.84TMNTDD36 pKa = 3.0AYY38 pKa = 10.49SAIVVDD44 pKa = 4.77DD45 pKa = 4.31AKK47 pKa = 11.29QNVGILDD54 pKa = 3.84SQSVIDD60 pKa = 4.55AYY62 pKa = 10.21KK63 pKa = 10.07EE64 pKa = 3.9ASSNLDD70 pKa = 4.03DD71 pKa = 5.72IYY73 pKa = 11.13MEE75 pKa = 5.52LDD77 pKa = 3.34DD78 pKa = 5.56EE79 pKa = 4.72MIEE82 pKa = 4.14EE83 pKa = 4.35GLDD86 pKa = 3.43YY87 pKa = 11.24VFAAMGYY94 pKa = 8.65YY95 pKa = 9.86YY96 pKa = 9.63EE97 pKa = 4.4TVYY100 pKa = 10.97QLLIGDD106 pKa = 4.29SNFEE110 pKa = 3.85AVYY113 pKa = 10.8LGDD116 pKa = 5.26DD117 pKa = 3.93SNQNSIDD124 pKa = 3.35GVTVLKK130 pKa = 9.42FTEE133 pKa = 4.26APEE136 pKa = 4.13IEE138 pKa = 4.35VEE140 pKa = 4.77LIEE143 pKa = 4.9IMNDD147 pKa = 2.71IARR150 pKa = 11.84EE151 pKa = 4.07EE152 pKa = 4.06IGEE155 pKa = 4.07AVYY158 pKa = 10.91VQDD161 pKa = 3.13NFMVVVNAEE170 pKa = 4.25YY171 pKa = 10.63INDD174 pKa = 3.36VDD176 pKa = 5.44AFLALAKK183 pKa = 9.87TGVASVIKK191 pKa = 10.44KK192 pKa = 10.15

Molecular weight: 21.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7Z8E4|A0A6G7Z8E4_9FIRM Recombination protein RecR OS=Erysipelothrix sp. HDW6B OX=2714929 GN=recR PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.95RR10 pKa = 11.84KK11 pKa = 7.81HH12 pKa = 5.11QKK14 pKa = 8.48VHH16 pKa = 5.53GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.28TVGGRR28 pKa = 11.84RR29 pKa = 11.84VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.63GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2061

0

2061

663539

25

5519

322.0

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.923 ± 0.058

0.53 ± 0.016

5.991 ± 0.051

6.463 ± 0.054

4.496 ± 0.048

6.365 ± 0.063

2.098 ± 0.034

8.216 ± 0.057

5.939 ± 0.052

9.068 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.934 ± 0.036

5.071 ± 0.043

3.292 ± 0.032

3.615 ± 0.032

3.972 ± 0.045

6.299 ± 0.042

6.331 ± 0.078

7.529 ± 0.053

0.802 ± 0.018

4.065 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski