Clostridium botulinum C phage (Clostridium botulinum C bacteriophage)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q331W9|Q331W9_CBCP Uncharacterized protein OS=Clostridium botulinum C phage OX=12336 GN=CST157 PE=4 SV=1
MM1 pKa = 7.42TSYY4 pKa = 10.82TSYY7 pKa = 11.7DD8 pKa = 3.62EE9 pKa = 5.45IFTCFLDD16 pKa = 3.42NCGIDD21 pKa = 3.73TDD23 pKa = 4.47NLPKK27 pKa = 10.75SNEE30 pKa = 3.54GKK32 pKa = 10.16YY33 pKa = 9.8QLIHH37 pKa = 6.3NAIIHH42 pKa = 6.01YY43 pKa = 7.58NTVIDD48 pKa = 4.43EE49 pKa = 4.79SYY51 pKa = 11.51TNLNYY56 pKa = 10.42DD57 pKa = 3.74DD58 pKa = 3.82EE59 pKa = 5.13KK60 pKa = 11.27EE61 pKa = 4.09RR62 pKa = 11.84VNILLDD68 pKa = 3.79NNQLLLLAYY77 pKa = 9.29CLRR80 pKa = 11.84YY81 pKa = 10.01SYY83 pKa = 11.28LEE85 pKa = 3.89NEE87 pKa = 4.46LIEE90 pKa = 5.82FEE92 pKa = 4.89EE93 pKa = 4.59LWQPFQKK100 pKa = 10.58EE101 pKa = 4.34VGQKK105 pKa = 10.02FYY107 pKa = 11.21RR108 pKa = 11.84EE109 pKa = 3.85QLQGRR114 pKa = 11.84EE115 pKa = 4.01STLARR120 pKa = 11.84TQNKK124 pKa = 9.51INEE127 pKa = 4.6LLNNMEE133 pKa = 4.72DD134 pKa = 3.44FDD136 pKa = 5.21YY137 pKa = 11.69NN138 pKa = 3.36

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q332A6|Q332A6_CBCP Uncharacterized protein OS=Clostridium botulinum C phage OX=12336 GN=CST120 PE=4 SV=1
MM1 pKa = 7.25NKK3 pKa = 9.52KK4 pKa = 10.52SKK6 pKa = 9.17VTVILVLVLSLLFSQVVFAKK26 pKa = 10.5SRR28 pKa = 11.84GSGGRR33 pKa = 11.84GRR35 pKa = 11.84RR36 pKa = 11.84SGNVSVRR43 pKa = 11.84GYY45 pKa = 8.87YY46 pKa = 10.24RR47 pKa = 11.84KK48 pKa = 10.27DD49 pKa = 2.91GTYY52 pKa = 10.31VRR54 pKa = 11.84PHH56 pKa = 5.26TRR58 pKa = 11.84SYY60 pKa = 11.11PSTHH64 pKa = 4.34GHH66 pKa = 6.65KK67 pKa = 9.49GTSYY71 pKa = 11.48SDD73 pKa = 3.42YY74 pKa = 10.18TYY76 pKa = 11.14DD77 pKa = 3.19SSLFISEE84 pKa = 4.53DD85 pKa = 3.21EE86 pKa = 4.19KK87 pKa = 11.78NEE89 pKa = 3.89LKK91 pKa = 10.81SRR93 pKa = 11.84EE94 pKa = 3.95KK95 pKa = 10.72SLKK98 pKa = 9.74KK99 pKa = 10.46EE100 pKa = 4.1KK101 pKa = 10.24IVNLL105 pKa = 3.76

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

192

0

192

49484

28

1662

257.7

29.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.47 ± 0.127

1.439 ± 0.095

6.162 ± 0.133

7.408 ± 0.201

4.003 ± 0.131

4.751 ± 0.132

1.461 ± 0.075

9.797 ± 0.203

11.262 ± 0.282

8.342 ± 0.127

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.066

8.607 ± 0.187

2.049 ± 0.102

3.654 ± 0.166

3.357 ± 0.126

5.875 ± 0.164

4.969 ± 0.12

4.994 ± 0.096

0.922 ± 0.051

5.165 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski