Puccinia triticina (isolate 1-1 / race 1 (BBBD)) (Brown leaf rust fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia; Puccinia triticina

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11515 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C4FBZ0|A0A0C4FBZ0_PUCT1 DEAD domain-containing protein OS=Puccinia triticina (isolate 1-1 / race 1 (BBBD)) OX=630390 PE=4 SV=1
LL1 pKa = 7.76DD2 pKa = 4.58LLKK5 pKa = 10.83HH6 pKa = 6.23SLLIDD11 pKa = 2.8NWTFFWILDD20 pKa = 3.79SLDD23 pKa = 3.47WILDD27 pKa = 3.65YY28 pKa = 11.1GYY30 pKa = 10.76SGYY33 pKa = 10.21IGLLDD38 pKa = 4.68FGLDD42 pKa = 4.29LDD44 pKa = 4.68TLDD47 pKa = 5.92SDD49 pKa = 4.82ILDD52 pKa = 4.49LAYY55 pKa = 9.49TWILLVLDD63 pKa = 3.64TGLWIWTATQDD74 pKa = 4.18FGFHH78 pKa = 6.26LLEE81 pKa = 4.51EE82 pKa = 4.48EE83 pKa = 4.17HH84 pKa = 5.97WTLDD88 pKa = 3.72FGLLDD93 pKa = 3.41STFFNSFWITLHH105 pKa = 6.82FGALPGDD112 pKa = 3.91RR113 pKa = 11.84QQ114 pKa = 3.31

Molecular weight: 13.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C4FCP3|A0A0C4FCP3_PUCT1 Integrase catalytic domain-containing protein OS=Puccinia triticina (isolate 1-1 / race 1 (BBBD)) OX=630390 PE=4 SV=1
MM1 pKa = 7.59APSIHH6 pKa = 6.69PAIAIATTTVHH17 pKa = 5.77SHH19 pKa = 7.09PPPSTRR25 pKa = 11.84TRR27 pKa = 11.84HH28 pKa = 5.75RR29 pKa = 11.84PLAPATVHH37 pKa = 5.68SHH39 pKa = 6.98PPPSTRR45 pKa = 11.84TRR47 pKa = 11.84HH48 pKa = 5.63RR49 pKa = 11.84PLANGTT55 pKa = 3.7

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11497

18

11515

4624077

49

4761

401.6

44.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.178 ± 0.024

1.284 ± 0.008

5.371 ± 0.016

5.626 ± 0.022

3.593 ± 0.014

6.035 ± 0.023

2.732 ± 0.014

4.883 ± 0.016

5.352 ± 0.025

9.37 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.883 ± 0.009

4.107 ± 0.014

7.273 ± 0.035

4.545 ± 0.017

5.722 ± 0.018

9.229 ± 0.032

5.954 ± 0.013

5.286 ± 0.018

1.268 ± 0.008

2.299 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski