Papilio machaon (Old World swallowtail butterfly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera;

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15461 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1I9A8|A0A0N1I9A8_PAPMA UDP-GlcNAc:betaGal beta-1 3-N-acetylglucosaminyltransferase-like protein 1 OS=Papilio machaon OX=76193 GN=RR48_02144 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 3.25SVTVSDD9 pKa = 4.46GDD11 pKa = 3.72SGVQVTDD18 pKa = 3.47SNRR21 pKa = 11.84GGIHH25 pKa = 6.68VDD27 pKa = 2.84GGDD30 pKa = 3.69YY31 pKa = 11.3GGVTAVDD38 pKa = 3.22NSGYY42 pKa = 10.71GGVSVADD49 pKa = 3.35NSGYY53 pKa = 11.0GGVHH57 pKa = 5.45VVDD60 pKa = 5.42DD61 pKa = 4.21SGQGGVNVVDD71 pKa = 4.6DD72 pKa = 4.55SGDD75 pKa = 3.38GGVNVVDD82 pKa = 4.43GSGHH86 pKa = 5.86FRR88 pKa = 11.84THH90 pKa = 6.18SAQVTSEE97 pKa = 4.1DD98 pKa = 4.4GEE100 pKa = 4.66GEE102 pKa = 4.21LNIVPDD108 pKa = 3.41SGFYY112 pKa = 9.56MPHH115 pKa = 7.18DD116 pKa = 3.78

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A194QN66|A0A194QN66_PAPMA Putative nuclease HARBI1 OS=Papilio machaon OX=76193 GN=RR48_14159 PE=3 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84AAQGHH7 pKa = 5.7APRR10 pKa = 11.84LSVARR15 pKa = 11.84GSAVTARR22 pKa = 11.84SPQSSAARR30 pKa = 11.84VYY32 pKa = 10.1VPRR35 pKa = 11.84GAFQPWPGRR44 pKa = 11.84RR45 pKa = 11.84SPGAGAHH52 pKa = 4.87TQGHH56 pKa = 5.86ARR58 pKa = 11.84HH59 pKa = 6.37RR60 pKa = 11.84SPALLAIQPRR70 pKa = 11.84NWILTFNKK78 pKa = 9.5RR79 pKa = 11.84RR80 pKa = 11.84SANN83 pKa = 3.26

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15461

0

15461

6951912

21

19906

449.6

50.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.184 ± 0.033

2.066 ± 0.039

5.489 ± 0.016

6.594 ± 0.025

3.499 ± 0.013

5.675 ± 0.034

2.53 ± 0.013

5.305 ± 0.019

6.244 ± 0.031

9.094 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.213 ± 0.01

4.852 ± 0.021

5.462 ± 0.029

3.879 ± 0.019

5.805 ± 0.022

7.596 ± 0.025

5.792 ± 0.022

6.352 ± 0.016

1.12 ± 0.008

3.25 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski