Epicoccum nigrum (Soil fungus) (Epicoccum purpurascens)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes;

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2LW69|A0A1Y2LW69_EPING Adenylate kinase OS=Epicoccum nigrum OX=105696 GN=ADK1 PE=3 SV=1
MM1 pKa = 7.51SSVSQIINFAGSFIVVCISAAFLITTIAEE30 pKa = 3.59IHH32 pKa = 6.33NILRR36 pKa = 11.84SLLVPAISEE45 pKa = 3.81LFYY48 pKa = 10.69ILRR51 pKa = 11.84SLGTLLPFFLYY62 pKa = 10.55DD63 pKa = 3.66CWEE66 pKa = 3.81SWLFRR71 pKa = 11.84EE72 pKa = 4.98LVNGNIDD79 pKa = 2.86IRR81 pKa = 11.84YY82 pKa = 6.97VANYY86 pKa = 10.04IIGQTLKK93 pKa = 10.6LFHH96 pKa = 6.86PLIEE100 pKa = 4.15VEE102 pKa = 4.23TWDD105 pKa = 3.74EE106 pKa = 4.14LDD108 pKa = 4.11YY109 pKa = 11.63LSDD112 pKa = 5.47DD113 pKa = 3.76DD114 pKa = 5.85WEE116 pKa = 4.73DD117 pKa = 3.78LSDD120 pKa = 6.2DD121 pKa = 3.89EE122 pKa = 6.98DD123 pKa = 4.5EE124 pKa = 5.97DD125 pKa = 6.05FSDD128 pKa = 5.81DD129 pKa = 3.68DD130 pKa = 3.56WW131 pKa = 6.53

Molecular weight: 15.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2LRF4|A0A1Y2LRF4_EPING Uncharacterized protein OS=Epicoccum nigrum OX=105696 GN=B5807_10382 PE=4 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.38QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TNNTIRR36 pKa = 11.84YY37 pKa = 5.73NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12021

0

12021

5572407

50

9372

463.6

51.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.132 ± 0.022

1.212 ± 0.01

5.676 ± 0.017

6.118 ± 0.026

3.635 ± 0.016

6.821 ± 0.025

2.445 ± 0.01

4.678 ± 0.017

4.962 ± 0.021

8.729 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.01

3.642 ± 0.013

6.028 ± 0.026

4.141 ± 0.02

6.037 ± 0.023

8.045 ± 0.026

6.124 ± 0.016

6.13 ± 0.017

1.465 ± 0.008

2.79 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski